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October-2015 Volume 34 Issue 4

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line

  • Authors:
    • Changhong Zhao
    • Sarra Setrerrahmane
    • Hanmei Xu
  • View Affiliations / Copyright

    Affiliations: The Engineering Research Center of Peptide Drug Discovery and Development, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 2126-2132
    |
    Published online on: July 30, 2015
       https://doi.org/10.3892/or.2015.4163
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Abstract

Tumor cells from the same origin comprise different cell populations. Among them, cancer stem cells (CSCs) have higher tumorigenicity. It is necessary to enrich CSCs to determine an effective way to suppress and eliminate them. In the present study, using the non-adhesive culture system, tumor spheres were successfully generated from human A549 non-small cell lung cancer (NSCLC) cell line within 2 weeks. Compared to A549 adherent cells, sphere cells had a higher self-renewal ability and increased resistance to cytotoxic drugs. Sphere cells were more invasive and expressed stem cell markers including octamer‑binding transcription factor 4 (Oct4) and sex-determining region Y-box 2 (Sox2) at high levels. CD133, a disputed marker of lung CSCs, was also upregulated. Tumor sphere cells showed higher tumorigenic ability in vivo, indicating that more CSCs were enriched in the sphere cells. More blood vessels were formed in the tumor generated by sphere cells suggesting the interaction between CSCs and blood vessel. A reliable model of enriching CSCs from the human A549 NSCLC cell line was established that was simple and cost-effective compared to other methods.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao C, Setrerrahmane S and Xu H: Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line. Oncol Rep 34: 2126-2132, 2015.
APA
Zhao, C., Setrerrahmane, S., & Xu, H. (2015). Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line. Oncology Reports, 34, 2126-2132. https://doi.org/10.3892/or.2015.4163
MLA
Zhao, C., Setrerrahmane, S., Xu, H."Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line". Oncology Reports 34.4 (2015): 2126-2132.
Chicago
Zhao, C., Setrerrahmane, S., Xu, H."Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line". Oncology Reports 34, no. 4 (2015): 2126-2132. https://doi.org/10.3892/or.2015.4163
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao C, Setrerrahmane S and Xu H: Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line. Oncol Rep 34: 2126-2132, 2015.
APA
Zhao, C., Setrerrahmane, S., & Xu, H. (2015). Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line. Oncology Reports, 34, 2126-2132. https://doi.org/10.3892/or.2015.4163
MLA
Zhao, C., Setrerrahmane, S., Xu, H."Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line". Oncology Reports 34.4 (2015): 2126-2132.
Chicago
Zhao, C., Setrerrahmane, S., Xu, H."Enrichment and characterization of cancer stem cells from a human non-small cell lung cancer cell line". Oncology Reports 34, no. 4 (2015): 2126-2132. https://doi.org/10.3892/or.2015.4163
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