miR-219-5p plays a tumor suppressive role in colon cancer by targeting oncogene Sall4

  • Authors:
    • Ji Cheng
    • Rui Deng
    • Peng Zhang
    • Chuanqing Wu
    • Ke Wu
    • Liang Shi
    • Xinghua Liu
    • Jie Bai
    • Meizhou Deng
    • Xiaoming Shuai
    • Jinbo Gao
    • Guobin Wang
    • Kaixiong Tao
  • View Affiliations

  • Published online on: August 4, 2015     https://doi.org/10.3892/or.2015.4168
  • Pages: 1923-1932
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Abstract

Sall4 is a novel oncogene found upregulated in several malignancies including colon cancer. However, its upstream regulatory miRNA is still undefined. miR-219-5p is regarded as a tumor-related miRNA in cancer research. Nevertheless, its actual role of whether inhibiting or promoting tumorigenesis is unclear in colon cancer. Potential interaction between Sall4 and miR-219-5p is predicted by TargetScan. CCK-8 test was used for evaluation of cell proliferation and cell survival rates. Western blot analysis and real-time PCR were applied for detection of target molecules. Luciferase assay was a direct confirmation of mutual interaction. Wound healing assay and transwell assay were conducted for cell migration and invasion tests. Flow cytometry was used for cell apoptosis analysis. Tissue specimens and cell lines were explored for miR-219-5p inhibition on colon cancer proliferation, migration, invasion, apoptosis and drug resistance by targeting Sall4. The results show that miR-219-5p inhibited carcinogenesis of colon cancer by targeting oncogene Sall4.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cheng J, Deng R, Zhang P, Wu C, Wu K, Shi L, Liu X, Bai J, Deng M, Shuai X, Shuai X, et al: miR-219-5p plays a tumor suppressive role in colon cancer by targeting oncogene Sall4. Oncol Rep 34: 1923-1932, 2015.
APA
Cheng, J., Deng, R., Zhang, P., Wu, C., Wu, K., Shi, L. ... Tao, K. (2015). miR-219-5p plays a tumor suppressive role in colon cancer by targeting oncogene Sall4. Oncology Reports, 34, 1923-1932. https://doi.org/10.3892/or.2015.4168
MLA
Cheng, J., Deng, R., Zhang, P., Wu, C., Wu, K., Shi, L., Liu, X., Bai, J., Deng, M., Shuai, X., Gao, J., Wang, G., Tao, K."miR-219-5p plays a tumor suppressive role in colon cancer by targeting oncogene Sall4". Oncology Reports 34.4 (2015): 1923-1932.
Chicago
Cheng, J., Deng, R., Zhang, P., Wu, C., Wu, K., Shi, L., Liu, X., Bai, J., Deng, M., Shuai, X., Gao, J., Wang, G., Tao, K."miR-219-5p plays a tumor suppressive role in colon cancer by targeting oncogene Sall4". Oncology Reports 34, no. 4 (2015): 1923-1932. https://doi.org/10.3892/or.2015.4168