MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo

  • Authors:
    • Wei Zhu
    • Jing Liu
    • Jihua Nie
    • Wenjiong Sheng
    • Han Cao
    • Wenhao Shen
    • Aijing Dong
    • Jundong Zhou
    • Yang Jiao
    • Shuyu Zhang
    • Jianping Cao
  • View Affiliations

  • Published online on: August 4, 2015     https://doi.org/10.3892/or.2015.4169
  • Pages: 2083-2089
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Abstract

Radiotherapy is a common treatment modality for lung cancer, however, radioresistance remains a fundamental barrier to attaining the maximal efficacy. Cancer cells take advantage of the ubiquitin-proteasome system (UPS) for increased proliferation and decreased apoptotic cell death. MG132 (carbobenzoxyl-leucinyl-leucinyl-leucinal‑H), a specific and selective reversible inhibitor of the 26S proteasome, has shown anticancer effect in multiple types of cancers. Previously, we have reported that MG132 enhances the anti‑growth and anti-metastatic effects of irradiation in lung cancer cells. However, whether MG132 can enhance the radiosensitivity in lung cancer cells in vitro and in vivo is still unknown. In this study, we found that MG132 increased apoptosis and dicentric chromosome ratio of A549 and H1299 cells treated by irradiation. Radiation-induced NF-κB expression and IκBα phosphorylation was attenuated in MG132 plus irradiation-treated cells. The in vivo model of H1299 xenografts of nude mice showed that the tumor size of MG132 plus irradiation treated xenografts was smaller than that of irradiation, MG132 or the control group. Moreover, MG132 plus irradiation group showed significant reduced Ki67 expression. Taken together, these results demonstrate that MG132 enhances the radiosensitivity through multiple mechanisms in vitro and in vivo.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhu W, Liu J, Nie J, Sheng W, Cao H, Shen W, Dong A, Zhou J, Jiao Y, Zhang S, Zhang S, et al: MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncol Rep 34: 2083-2089, 2015
APA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W. ... Cao, J. (2015). MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncology Reports, 34, 2083-2089. https://doi.org/10.3892/or.2015.4169
MLA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34.4 (2015): 2083-2089.
Chicago
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34, no. 4 (2015): 2083-2089. https://doi.org/10.3892/or.2015.4169