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Article

MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo

  • Authors:
    • Wei Zhu
    • Jing Liu
    • Jihua Nie
    • Wenjiong Sheng
    • Han Cao
    • Wenhao Shen
    • Aijing Dong
    • Jundong Zhou
    • Yang Jiao
    • Shuyu Zhang
    • Jianping Cao
  • View Affiliations / Copyright

    Affiliations: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China, The Core Laboratory of Suzhou Cancer Center and Department of Radiotherapy of Suzhou Municipal Hospital, Suzhou, Jiangsu 215001, P.R. China
  • Pages: 2083-2089
    |
    Published online on: August 4, 2015
       https://doi.org/10.3892/or.2015.4169
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Abstract

Radiotherapy is a common treatment modality for lung cancer, however, radioresistance remains a fundamental barrier to attaining the maximal efficacy. Cancer cells take advantage of the ubiquitin-proteasome system (UPS) for increased proliferation and decreased apoptotic cell death. MG132 (carbobenzoxyl-leucinyl-leucinyl-leucinal‑H), a specific and selective reversible inhibitor of the 26S proteasome, has shown anticancer effect in multiple types of cancers. Previously, we have reported that MG132 enhances the anti‑growth and anti-metastatic effects of irradiation in lung cancer cells. However, whether MG132 can enhance the radiosensitivity in lung cancer cells in vitro and in vivo is still unknown. In this study, we found that MG132 increased apoptosis and dicentric chromosome ratio of A549 and H1299 cells treated by irradiation. Radiation-induced NF-κB expression and IκBα phosphorylation was attenuated in MG132 plus irradiation-treated cells. The in vivo model of H1299 xenografts of nude mice showed that the tumor size of MG132 plus irradiation treated xenografts was smaller than that of irradiation, MG132 or the control group. Moreover, MG132 plus irradiation group showed significant reduced Ki67 expression. Taken together, these results demonstrate that MG132 enhances the radiosensitivity through multiple mechanisms in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu W, Liu J, Nie J, Sheng W, Cao H, Shen W, Dong A, Zhou J, Jiao Y, Zhang S, Zhang S, et al: MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncol Rep 34: 2083-2089, 2015.
APA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W. ... Cao, J. (2015). MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncology Reports, 34, 2083-2089. https://doi.org/10.3892/or.2015.4169
MLA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34.4 (2015): 2083-2089.
Chicago
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34, no. 4 (2015): 2083-2089. https://doi.org/10.3892/or.2015.4169
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu W, Liu J, Nie J, Sheng W, Cao H, Shen W, Dong A, Zhou J, Jiao Y, Zhang S, Zhang S, et al: MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncol Rep 34: 2083-2089, 2015.
APA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W. ... Cao, J. (2015). MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo. Oncology Reports, 34, 2083-2089. https://doi.org/10.3892/or.2015.4169
MLA
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34.4 (2015): 2083-2089.
Chicago
Zhu, W., Liu, J., Nie, J., Sheng, W., Cao, H., Shen, W., Dong, A., Zhou, J., Jiao, Y., Zhang, S., Cao, J."MG132 enhances the radiosensitivity of lung cancer cells in vitro and in vivo". Oncology Reports 34, no. 4 (2015): 2083-2089. https://doi.org/10.3892/or.2015.4169
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