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Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product

  • Authors:
    • Mamdooh Ghoneum
    • Nouran Felo
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Charles R. Drew University of Medicine and Science, Los Angeles, CA 90059, USA
    Copyright: © Ghoneum et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1659-1666
    |
    Published online on: August 6, 2015
       https://doi.org/10.3892/or.2015.4180
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Abstract

The present study was undertaken to evaluate the effect of Lactobacillus kefiri (PFT), a novel kefir product, on apoptosis of gastric cancer cells (AGS), breast cancer cells (4T1), and human peripheral blood mononuclear cells (PBMCs). Cells were cultured with PFT and apoptosis was determined by flow cytometry using 7-AAD dye and cytospin preparation. Mitochondrial dysfunction and expression of Bcl2 were monitored by flow cytometry. Results showed that PFT induced apoptosis in AGS gastric cancer cells in a dose-dependent manner. Apoptosis was detected at a concentration of 0.3 mg/ml (20.8%), increased to 25.8% at 0.6 mg/ml, 37% at 1.2 mg/ml, 53.1% at 2.5 mg/ml, and peaked at 66.3% at 5.0 mg/ml. Apoptosis is associated with the decreased polarization of mitochondrial membrane potential (MMP) and decreased Bcl2 expression. PFT-treated AGS cells manifested membrane blebbing, nuclear condensation, and fragmentation as identified in cytospin cytocentrifuge Giemsa stained preparations. On the other hand, flow cytometry analysis showed that PFT did not induce apoptosis in 4T1 breast cancer cells nor in PBMCs. These results suggest that PFT is safe for white blood cells and selectively induces apoptotic effects in gastric cancer cells. Hence, it may have potential as a therapeutic agent for the treatment of gastric cancers.
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Copy and paste a formatted citation
Spandidos Publications style
Ghoneum M and Felo N: Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product. Oncol Rep 34: 1659-1666, 2015.
APA
Ghoneum, M., & Felo, N. (2015). Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product. Oncology Reports, 34, 1659-1666. https://doi.org/10.3892/or.2015.4180
MLA
Ghoneum, M., Felo, N."Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product". Oncology Reports 34.4 (2015): 1659-1666.
Chicago
Ghoneum, M., Felo, N."Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product". Oncology Reports 34, no. 4 (2015): 1659-1666. https://doi.org/10.3892/or.2015.4180
Copy and paste a formatted citation
x
Spandidos Publications style
Ghoneum M and Felo N: Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product. Oncol Rep 34: 1659-1666, 2015.
APA
Ghoneum, M., & Felo, N. (2015). Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product. Oncology Reports, 34, 1659-1666. https://doi.org/10.3892/or.2015.4180
MLA
Ghoneum, M., Felo, N."Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product". Oncology Reports 34.4 (2015): 1659-1666.
Chicago
Ghoneum, M., Felo, N."Selective induction of apoptosis in human gastric cancer cells by Lactobacillus kefiri (PFT), a novel kefir product". Oncology Reports 34, no. 4 (2015): 1659-1666. https://doi.org/10.3892/or.2015.4180
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