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Article

Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells

  • Authors:
    • Masayoshi Yamaguchi
    • Tatyana Vikulina
    • M. Neale Weitzmann
  • View Affiliations / Copyright

    Affiliations: Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA, Division of Endocrinology and Metabolism and Lipids, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
  • Pages: 2156-2162
    |
    Published online on: August 10, 2015
       https://doi.org/10.3892/or.2015.4190
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Abstract

Gentian violet (GV) is a cationic triphenylmethane dye, with potent antifungal and antibacterial activity. We recently reported that in vitro GV suppresses the differentiation of bone resorbing osteoclasts while stimulating the differentiation and activity of bone forming osteoblasts. Breast cancer is highly metastatic to bone and drives bone turnover that further promotes cancer engraftment and expansion, the so-called vicious cycle. In humans, breast cancer metastases cause osteolytic lesions and skeletal damage that leads to bone fractures, an additional source of patient morbidity. The MDA-MB-231 human breast cancer cell line is a commonly used model of human breast cancer that when injected into mice metastasizes to bone causing osteolytic lesions by promoting osteoclastic bone resorption and/or suppressing osteoblastic bone formation. In the present study, we investigated the direct action of GV on MDA-MB-231 proliferation, and the capacity of GV to reverse the negative impact of MDA-MB-231 cells on osteoclast and osteoblast differentiation. Our data reveal for the first time that GV suppresses proliferation, and induces apoptosis, of MDA-MB-231 cells. We further demonstrated the capacity of GV to reverse the pro-osteoclastogenic and anti-osteoblastic activities of MDA-MB-231 cells in vitro. These data suggest that GV has important applications in the treatment of breast cancer through multiple actions including direct suppression of cancer cell proliferation, breaking the vicious cycle between cancer and bone, and alleviating the skeletal defects induced by bone metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Yamaguchi M, Vikulina T and Weitzmann MN: Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells. Oncol Rep 34: 2156-2162, 2015.
APA
Yamaguchi, M., Vikulina, T., & Weitzmann, M.N. (2015). Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells. Oncology Reports, 34, 2156-2162. https://doi.org/10.3892/or.2015.4190
MLA
Yamaguchi, M., Vikulina, T., Weitzmann, M. N."Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells". Oncology Reports 34.4 (2015): 2156-2162.
Chicago
Yamaguchi, M., Vikulina, T., Weitzmann, M. N."Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells". Oncology Reports 34, no. 4 (2015): 2156-2162. https://doi.org/10.3892/or.2015.4190
Copy and paste a formatted citation
x
Spandidos Publications style
Yamaguchi M, Vikulina T and Weitzmann MN: Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells. Oncol Rep 34: 2156-2162, 2015.
APA
Yamaguchi, M., Vikulina, T., & Weitzmann, M.N. (2015). Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells. Oncology Reports, 34, 2156-2162. https://doi.org/10.3892/or.2015.4190
MLA
Yamaguchi, M., Vikulina, T., Weitzmann, M. N."Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells". Oncology Reports 34.4 (2015): 2156-2162.
Chicago
Yamaguchi, M., Vikulina, T., Weitzmann, M. N."Gentian violet inhibits MDA-MB-231 human breast cancer cell proliferation, and reverses the stimulation of osteoclastogenesis and suppression of osteoblast activity induced by cancer cells". Oncology Reports 34, no. 4 (2015): 2156-2162. https://doi.org/10.3892/or.2015.4190
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