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Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation

  • Authors:
    • Omar S. El-Masry
    • Kaltoom Al-Sakkaf
    • Barry L. Brown
    • Pauline R.M. Dobson
  • View Affiliations / Copyright

    Affiliations: Department of Human Metabolism, The Medical School, University of Sheffield, Sheffield, S10 2RX, UK
    Copyright: © El-Masry et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1675-1680
    |
    Published online on: August 11, 2015
       https://doi.org/10.3892/or.2015.4198
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Abstract

AMP-activated protein kinase (AMPK), a sensor of cellular energy, is widely reported as a potential therapeutic target in treatment of breast and other cancers. The activated enzyme has been shown to be a promising anti-proliferative agent in breast cancer cell lines. However, little data exist on crosstalk between AMPK and the cellular survival axis of PI3K/Akt/mTOR pathway and the impact of microenvironment on cellular responses to AMPK activation. We present results which show differential crosstalk between AMPK and Akt, dependent on the cellular genetics of each breast cancer cell type. We also show that leptin blocks activation of AMPK and partially or completely attenuates the anti-proliferative effect of AMPK activation depending on the cell type. This suggests that leptin within the local environment might impose limitations on therapeutic usage of AMPK activators in cancer, thereby attenuating their effective use in many obese subjects.
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Copy and paste a formatted citation
Spandidos Publications style
El-Masry OS, Al-Sakkaf K, Brown BL and Dobson PR: Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation. Oncol Rep 34: 1675-1680, 2015.
APA
El-Masry, O.S., Al-Sakkaf, K., Brown, B.L., & Dobson, P.R. (2015). Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation. Oncology Reports, 34, 1675-1680. https://doi.org/10.3892/or.2015.4198
MLA
El-Masry, O. S., Al-Sakkaf, K., Brown, B. L., Dobson, P. R."Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation". Oncology Reports 34.4 (2015): 1675-1680.
Chicago
El-Masry, O. S., Al-Sakkaf, K., Brown, B. L., Dobson, P. R."Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation". Oncology Reports 34, no. 4 (2015): 1675-1680. https://doi.org/10.3892/or.2015.4198
Copy and paste a formatted citation
x
Spandidos Publications style
El-Masry OS, Al-Sakkaf K, Brown BL and Dobson PR: Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation. Oncol Rep 34: 1675-1680, 2015.
APA
El-Masry, O.S., Al-Sakkaf, K., Brown, B.L., & Dobson, P.R. (2015). Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation. Oncology Reports, 34, 1675-1680. https://doi.org/10.3892/or.2015.4198
MLA
El-Masry, O. S., Al-Sakkaf, K., Brown, B. L., Dobson, P. R."Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation". Oncology Reports 34.4 (2015): 1675-1680.
Chicago
El-Masry, O. S., Al-Sakkaf, K., Brown, B. L., Dobson, P. R."Differential crosstalk between the AMPK and PI3K/Akt pathways in breast cancer cells of differing genotypes: Leptin inhibits the effectiveness of AMPK activation". Oncology Reports 34, no. 4 (2015): 1675-1680. https://doi.org/10.3892/or.2015.4198
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