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Article

Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells

  • Authors:
    • Hisashi Takeuchi
    • Chinedu O. Mmeje
    • Goodwin G. Jinesh
    • Rikiya Taoka
    • Ashish M. Kamat
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
  • Pages: 2738-2744
    |
    Published online on: August 21, 2015
       https://doi.org/10.3892/or.2015.4220
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Abstract

Bladder cancer is a common malignancy for which regional or metastatic disease is identified at diagnosis. The aim of this study was to determine whether tamoxifen (Tam), an estrogen receptor (ER) antagonist, can sensitize bladder cancer cell lines to gemcitabine (Gem) chemotherapy. ERα and ERβ protein levels were determined in each cell line using western blot analysis. The TCC-Sup, 5637, and RT4 bladder cancer cells were exposed to various concentrations and regimens of Tam or Gem alone or in combination. Cell viability and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and propidium iodide followed by flow cytometry. Apoptosis was then evaluated by western blot analysis. Treated TCC-Sup cells were subjected to soft agar colony formation assay to determine the cellular transformation. Western blot analysis results revealed ER expression in the three cell lines. TCC-Sup and 5637 cells treated with a combination of Tam and Gem had lower cell viabilities than those treated with Tam or Gem alone for 72 h in TCC-Sup and 5637. Compared with the other treatments, sequential Gem followed by Tam (Gem→Tam) treatment caused the largest increase in DNA fragmentation at 72 h in TCC-Sup cells. Western blot analysis results revealed that this sequential Gem→Tam treatment increased poly(ADP-ribose) polymerase cleavage in TCC-Sup cells. Sequential Gem→Tam inhibited the cell transformation in TCC-Sup cells. In conclusion, sequential Gem→Tam enhanced the cytotoxicity of Gem in vitro. This regimen be useful to enhance the efficacy of Gem in bladder cancer. However, future in vivo studies are required to verify the results.
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1 

DeSantis CE, Lin CC, Mariotto AB, Siegel RL, Stein KD, Kramer JL, Alteri R, Robbins AS and Jemal A: Cancer treatment and survivorship statistics, 2014. CA Cancer J Clin. 64:252–271. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Rosenberg JE, Carroll PR and Small EJ: Update on chemotherapy for advanced bladder cancer. J Urol. 174:14–20. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Stein JP, Lieskovsky G, Cote R, Groshen S, Feng AC, Boyd S, Skinner E, Bochner B, Thangathurai D, Mikhail M, et al: Radical cystectomy in the treatment of invasive bladder cancer: Long-term results in 1,054 patients. J Clin Oncol. 19:666–675. 2001.PubMed/NCBI

4 

Galsky MD, Moshier E, Krege S, Lin CC, Hahn N, Ecke T, Sonpavde G, Godbold J, Oh WK and Bamias A: Nomogram for predicting survival in patients with unresectable and/or metastatic urothelial cancer who are treated with cisplatin-based chemotherapy. Cancer. 119:3012–3019. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Sternberg CN, Yagoda A, Scher HI, Watson RC, Geller N, Herr HW, Morse MJ, Sogani PC, Vaughan ED, Bander N, et al: Methotrexate, vinblastine, doxorubicin, and cisplatin for advanced transitional cell carcinoma of the urothelium. Efficacy and patterns of response and relapse. Cancer. 64:2448–2458. 1989. View Article : Google Scholar : PubMed/NCBI

6 

von der Maase H, Hansen SW, Roberts JT, Dogliotti L, Oliver T, Moore MJ, Bodrogi I, Albers P, Knuth A, Lippert CM, et al: Gemcitabine and cisplatin versus methotrexate, vinblastine, doxorubicin, and cisplatin in advanced or metastatic bladder cancer: Results of a large, randomized, multinational, multi-center, phase III study. J Clin Oncol. 18:3068–3077. 2000.PubMed/NCBI

7 

Hussain M, Vaishampayan U, Du W, Redman B and Smith DC: Combination paclitaxel, carboplatin, and gemcitabine is an active treatment for advanced urothelial cancer. J Clin Oncol. 19:2527–2533. 2001.PubMed/NCBI

8 

Choi W, Porten S, Kim S, Willis D, Plimack ER, Hoffman-Censits J, Roth B, Cheng T, Tran M, Lee IL, et al: Identification of distinct basal and luminal subtypes of muscle-invasive bladder cancer with different sensitivities to frontline chemotherapy. Cancer Cell. 25:152–165. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Nilsson S, Mäkelä S, Treuter E, Tujague M, Thomsen J, Andersson G, Enmark E, Pettersson K, Warner M and Gustafsson JA: Mechanisms of estrogen action. Physiol Rev. 81:1535–1565. 2001.PubMed/NCBI

10 

Harris HA: Estrogen receptor-beta: Recent lessons from in vivo studies. Mol Endocrinol. 21:1–13. 2007. View Article : Google Scholar

11 

Miyamoto H, Yao JL, Chaux A, Zheng Y, Hsu I, Izumi K, Chang C, Messing EM, Netto GJ and Yeh S: Expression of androgen and oestrogen receptors and its prognostic significance in urothelial neoplasm of the urinary bladder. BJU Int. 109:1716–1726. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Shen SS, Smith CL, Hsieh JT, Yu J, Kim IY, Jian W, Sonpavde G, Ayala GE, Younes M and Lerner SP: Expression of estrogen receptors-alpha and -beta in bladder cancer cell lines and human bladder tumor tissue. Cancer. 106:2610–2616. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Tuygun C, Kankaya D, Imamoglu A, Sertcelik A, Zengin K, Oktay M and Sertcelik N: Sex-specific hormone receptors in urothelial carcinomas of the human urinary bladder: A comparative analysis of clinicopathological features and survival outcomes according to receptor expression. Urol Oncol. 29:43–51. 2011. View Article : Google Scholar

14 

Pfister C: New trends for optimal management of bladder tumors. J Urol. 165:65–66. 2001. View Article : Google Scholar

15 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Zheng A, Kallio A and Härkönen P: Tamoxifen-induced rapid death of MCF-7 breast cancer cells is mediated via extracellularly signal-regulated kinase signaling and can be abrogated by estrogen. Endocrinology. 148:2764–2777. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Jinesh GG, Choi W, Shah JB, Lee EK, Willis DL and Kamat AM: Blebbishields, the emergency program for cancer stem cells: Sphere formation and tumorigenesis after apoptosis. Cell Death Differ. 20:382–395. 2013. View Article : Google Scholar :

18 

von der Maase H, Sengelov L, Roberts JT, Ricci S, Dogliotti L, Oliver T, Moore MJ, Zimmermann A and Arning M: Long-term survival results of a randomized trial comparing gemcitabine plus cisplatin, with methotrexate, vinblastine, doxorubicin, plus cisplatin in patients with bladder cancer. J Clin Oncol. 23:4602–4608. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Huang P, Chubb S, Hertel LW, Grindey GB and Plunkett W: Action of 2′,2′-difluorodeoxycytidine on DNA synthesis. Cancer Res. 51:6110–6117. 1991.PubMed/NCBI

20 

Brauch H and Jordan VC: Targeting of tamoxifen to enhance anti-tumour action for the treatment and prevention of breast cancer: The 'personalised' approach? Eur J Cancer. 45:2274–2283. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Marshall E: Tamoxifen. 'A big deal,' but a complex hand to play. Science. 280:1961998. View Article : Google Scholar : PubMed/NCBI

22 

Silvestris N, Cinieri S, La Torre I, Pezzella G, Numico G, Orlando L and Lorusso V: Role of gemcitabine in metastatic breast cancer patients: A short review. Breast. 17:220–226. 2008. View Article : Google Scholar

23 

Cosco D, Paolino D, Cilurzo F, Casale F and Fresta M: Gemcitabine and tamoxifen-loaded liposomes as multidrug carriers for the treatment of breast cancer diseases. Int J Pharm. 422:229–237. 2012. View Article : Google Scholar

24 

Han B, Cui D, Jing Y, Hong Y and Xia S: Estrogen receptor β (ERβ) is a novel prognostic marker of recurrence survival in non-muscle-invasive bladder cancer potentially by inhibiting cadherin switch. World J Urol. 30:861–867. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Takeuchi H, Mmeje CO, Jinesh GG, Taoka R and Kamat AM: Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells. Oncol Rep 34: 2738-2744, 2015.
APA
Takeuchi, H., Mmeje, C.O., Jinesh, G.G., Taoka, R., & Kamat, A.M. (2015). Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells. Oncology Reports, 34, 2738-2744. https://doi.org/10.3892/or.2015.4220
MLA
Takeuchi, H., Mmeje, C. O., Jinesh, G. G., Taoka, R., Kamat, A. M."Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells". Oncology Reports 34.5 (2015): 2738-2744.
Chicago
Takeuchi, H., Mmeje, C. O., Jinesh, G. G., Taoka, R., Kamat, A. M."Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells". Oncology Reports 34, no. 5 (2015): 2738-2744. https://doi.org/10.3892/or.2015.4220
Copy and paste a formatted citation
x
Spandidos Publications style
Takeuchi H, Mmeje CO, Jinesh GG, Taoka R and Kamat AM: Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells. Oncol Rep 34: 2738-2744, 2015.
APA
Takeuchi, H., Mmeje, C.O., Jinesh, G.G., Taoka, R., & Kamat, A.M. (2015). Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells. Oncology Reports, 34, 2738-2744. https://doi.org/10.3892/or.2015.4220
MLA
Takeuchi, H., Mmeje, C. O., Jinesh, G. G., Taoka, R., Kamat, A. M."Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells". Oncology Reports 34.5 (2015): 2738-2744.
Chicago
Takeuchi, H., Mmeje, C. O., Jinesh, G. G., Taoka, R., Kamat, A. M."Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells". Oncology Reports 34, no. 5 (2015): 2738-2744. https://doi.org/10.3892/or.2015.4220
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