Hexane extract from Sargassum serratifolium inhibits the cell proliferation and metastatic ability of human glioblastoma U87MG cells

Kang,Chang-Won; Park,Min-Seok; Kim,Nan-Hee; Lee,Ji-Hyun; Oh,Chul-Woong; Kim,Hyeung-Rak; Kim,Gun-Do

doi:10.3892/or.2015.4222

Hexane extract from Sargassum serratifolium inhibits the cell proliferation and metastatic ability of human glioblastoma U87MG cells

Authors:
- Chang-Won Kang
- Min-Seok Park
- Nan-Hee Kim
- Ji-Hyun Lee
- Chul-Woong Oh
- Hyeung-Rak Kim
- Gun-Do Kim
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Affiliations: Department of Microbiology, College of Natural Science, Pukyong National University, Busan 608-737, Republic of Korea, Department of Marine Biology, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea, Department of Food Science and Nutrition, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea
Published online on: August 21, 2015 https://doi.org/10.3892/or.2015.4222
Pages: 2602-2608

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Abstract

The present study is the first to demonstrate the anticancer effects of a hexane extract from the brown algae Sargassum serratifolium (HES) on human cancer cell lines, including glioblastoma U87MG, cervical cancer HeLa and gastric cancer MKN-28 cells, as well as liver cancer SK-HEP 1 cells. Among these cancer cell lines, U87MG cells were most sensitive to the cell death induced by HES. HES exhibited a cytotoxic effect on U87MG cells at concentrations of 14-16 µg/ml, yet an effect was not observed in human embryonic kidney HEK293 cells. The antiproliferative effects of HES were regulated by inhibition of the MAPK/ERK signaling pathway which plays a pivotal role in the proliferation of glioblastoma U87MG cells. In addition, treatment with HES led to cell morphological changes and cell cytoskeleton degradation through regulation of actin dynamic signaling. Furthermore, migration and invasion of the U87MG cells were inhibited by HES via suppression of matrix metalloproteinase (MMP)-2 and -9 expression. Thus, our results suggest that HES is a potential therapeutic agent which has anticancer effects on glioblastoma.

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