Genistein enhances TRAIL-induced cancer cell death via inactivation of autophagic flux

  • Authors:
    • Uddin Md. Nazim
    • Sang-Youel Park
  • View Affiliations

  • Published online on: September 7, 2015     https://doi.org/10.3892/or.2015.4247
  • Pages: 2692-2698
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a transmembrane cytokine that is a promising anticancer agent as it selectively induces apoptosis in various types of tumor cells. Autophagic flux, which includes the complete process of autophagy, and suppression of autophagic flux has been increasingly recognized as a favorable and novel therapeutic approach for cancer treatment. Here, we showed that genistein, a major isoflavone compound that exerts its anticancer properties by inhibiting tumor cell proliferation, can induce TRAIL-mediated apoptotic cell death in TRAIL‑resistant human adenocarcinoma A549 cells. Notably, genistein treatment led to a marked increase in the accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II and p62 protein levels. The combination of genistein and TRAIL increased LC3-II, p62, activated caspase-3 and activated caspase-8 accumulation, confirming the inhibition of autophagic flux. Taken together, our results revealed that genistein enhanced TRAIL-induced tumor cell death in TRAIL-resistant A549 adenocarcinoma cells by inhibiting autophagic flux.
View Figures
View References

Related Articles

Journal Cover

November-2015
Volume 34 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Nazim UM and Park S: Genistein enhances TRAIL-induced cancer cell death via inactivation of autophagic flux. Oncol Rep 34: 2692-2698, 2015.
APA
Nazim, U.M., & Park, S. (2015). Genistein enhances TRAIL-induced cancer cell death via inactivation of autophagic flux. Oncology Reports, 34, 2692-2698. https://doi.org/10.3892/or.2015.4247
MLA
Nazim, U. M., Park, S."Genistein enhances TRAIL-induced cancer cell death via inactivation of autophagic flux". Oncology Reports 34.5 (2015): 2692-2698.
Chicago
Nazim, U. M., Park, S."Genistein enhances TRAIL-induced cancer cell death via inactivation of autophagic flux". Oncology Reports 34, no. 5 (2015): 2692-2698. https://doi.org/10.3892/or.2015.4247