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Article

Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells

  • Authors:
    • Chen Zhang
    • Yaping Tian
    • Feiyu Song
    • Changhao Fu
    • Bo Han
    • Yi Wang
  • View Affiliations / Copyright

    Affiliations: Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Dermatology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Jilin Connell Pharmaceutical Company, Changchun, Jilin 130000, P.R. China
  • Pages: 2469-2476
    |
    Published online on: September 8, 2015
       https://doi.org/10.3892/or.2015.4253
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Abstract

Salinomycin is a monocarboxylic polyether antibiotic that has been reported to induce apoptosis in various types of cancer cells with specificity for cancer stem cells. However, its anticancer effect in colorectal cancer stem cells has never been reported. In the present study, we examined the ability of salinomycin to induce cell death in the colorectal cancer stem cell line CD44+EpCAM+ HCT-116, and we measured its in vivo tumor inhibition capacity. Salinomycin dose-dependently induced cytotoxicity in the CD44+EpCAM+ HCT-116 cells and inhibited colony formation. Salinomycin treatment was shown to induce apoptosis, as evidenced by nuclear fragmentation, an increase in the proportion of acridine orange/ethidium bromide-positive cells and an increase in the percentage of Annexin V-positive cells. Apoptosis was induced in colorectal cancer stem cells in a caspase-dependent manner, as shown by an increase in the levels of cleaved caspase-3, -8 and -9. JC-1 staining further revealed that salinomycin induced colorectal cancer cell apoptosis via the mitochondrial pathway. In addition, salinomycin treatment of xenograft mice inhibited the growth of tumors derived from the CD44+EpCAM+ HCT-116 cells. The present study demonstrated that the antibiotic salinomycin exerts an anti-colorectal cancer effect in vitro and in vivo, suggesting salinomycin as a potential drug for colorectal cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Tian Y, Song F, Fu C, Han B and Wang Y: Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells. Oncol Rep 34: 2469-2476, 2015.
APA
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., & Wang, Y. (2015). Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells. Oncology Reports, 34, 2469-2476. https://doi.org/10.3892/or.2015.4253
MLA
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., Wang, Y."Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells". Oncology Reports 34.5 (2015): 2469-2476.
Chicago
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., Wang, Y."Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells". Oncology Reports 34, no. 5 (2015): 2469-2476. https://doi.org/10.3892/or.2015.4253
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Tian Y, Song F, Fu C, Han B and Wang Y: Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells. Oncol Rep 34: 2469-2476, 2015.
APA
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., & Wang, Y. (2015). Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells. Oncology Reports, 34, 2469-2476. https://doi.org/10.3892/or.2015.4253
MLA
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., Wang, Y."Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells". Oncology Reports 34.5 (2015): 2469-2476.
Chicago
Zhang, C., Tian, Y., Song, F., Fu, C., Han, B., Wang, Y."Salinomycin inhibits the growth of colorectal carcinoma by targeting tumor stem cells". Oncology Reports 34, no. 5 (2015): 2469-2476. https://doi.org/10.3892/or.2015.4253
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