Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma

  • Authors:
    • Ziling Huang
    • Lanshan Huang
    • Siqiao Shen
    • Jia Li
    • Huiping Lu
    • Weijia Mo
    • Yiwu Dang
    • Dianzhong Luo
    • Gang Chen
    • Zhenbo Feng
  • View Affiliations

  • Published online on: September 8, 2015     https://doi.org/10.3892/or.2015.4259
  • Pages: 2403-2412
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Abstract

Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), also known as nuclear-enriched transcript 2 (NEAT2), is highly conserved among mammals and highly expressed in the nucleus. It was first identified in lung cancer as a prognostic marker for metastasis but is also associated with several other solid tumors. In hepatocellular carcinoma (HCC), MALAT1 is a novel biomarker for predicting tumor recurrence after liver transplantation. The mechanism of overexpression in tumor progression remains unclear. In the present study, we investigated the role of specificity protein 1/3 (Sp1/3) in regulation of MALAT1 transcription in HCC cells. The results showed a high expression of Sp1, Sp3 and MALAT1 in HCC vs. paired non-tumor liver tissues, which was associated with the AFP level (Sp1, r=7.44, P=0.0064; MALAT1, r=12.37, P=0.0004). Co-silencing of Sp1 and Sp3 synergistically repressed MALAT1 expression. Sp1 binding inhibitor, mithramycin A (MIT), also inhibited MALAT1 expression in HCC cells. In conclusion, the upstream of MALAT1 contains five Sp1/3 binding sites, which may be responsible for MALAT1 transcription. Inhibitors, such as MIT, provide a potential therapeutic strategy for HCC patients with MALAT1 overexpression.
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November-2015
Volume 34 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang Z, Huang L, Shen S, Li J, Lu H, Mo W, Dang Y, Luo D, Chen G, Feng Z, Feng Z, et al: Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma. Oncol Rep 34: 2403-2412, 2015
APA
Huang, Z., Huang, L., Shen, S., Li, J., Lu, H., Mo, W. ... Feng, Z. (2015). Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma. Oncology Reports, 34, 2403-2412. https://doi.org/10.3892/or.2015.4259
MLA
Huang, Z., Huang, L., Shen, S., Li, J., Lu, H., Mo, W., Dang, Y., Luo, D., Chen, G., Feng, Z."Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma". Oncology Reports 34.5 (2015): 2403-2412.
Chicago
Huang, Z., Huang, L., Shen, S., Li, J., Lu, H., Mo, W., Dang, Y., Luo, D., Chen, G., Feng, Z."Sp1 cooperates with Sp3 to upregulate MALAT1 expression in human hepatocellular carcinoma". Oncology Reports 34, no. 5 (2015): 2403-2412. https://doi.org/10.3892/or.2015.4259