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Article

Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis

  • Authors:
    • Shanghui Zhou
    • Ling Chen
    • Mubarak Mashrah
    • Yun Zhu
    • Zhijing He
    • Yuhua Hu
    • Tingxiu Xiang
    • Zhigang Yao
    • Feng Guo
    • Chenping Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China, Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China, Department of Oral Pathology, Xiangya Stomatological Hospital, Central South University, Changsha, Hunan 410078, P.R. China, Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 2636-2642
    |
    Published online on: September 9, 2015
       https://doi.org/10.3892/or.2015.4264
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Abstract

Oral squamous cell carcinoma (OSCC) is a type of head and neck malignancy with a high mortality rate. Oral submucous fibrosis (OSF) is the pre-cancerous lesion of OSCC, whose molecular mechanisms in OSCC tumorigenesis remain largely unclear. Activation of the Wnt/β-catenin signaling pathway plays an important role in oral mucous carcinogenesis, although rare mutations of Wnt signaling molecules are found in OSCC, suggesting an epigenetic mechanism mediating aberrant Wnt/β‑catenin signaling in OSCC. Wnt inhibitory factor-1 (WIF1) is an Wnt antagonist, and its downregulation and methylation have been reported in a number of malignancies. However, the expression and methylation of WIF1 in the development of OSF have yet to be reported. In the present study, we investigated the WIF1 expression level by immuno­histochemical staining and semi‑quantitative RT-PCR in normal oral, OSF and OSCC tissues, as well as the methylation status by methylation-specific PCR and bisulfite genomic sequencing. The results showed that WIF1 was readily expressed in normal oral mucous tissues, but decreased gradually in OSF early, moderately advanced and advanced tissues, and was less expressed in OSCC tissues. Moreover, WIF1 was able to translocate from the nuclear to cytoplasm in OSF and OSCC tissues. Furthermore, WIF1 was frequently methylated in OSCC cases with betel quid chewing habit, but not in normal oral mucous and different stages of OSF tissues, suggesting WIF1 methylation is tumor-specific in the development of OSF. Thus, the results demonstrated that WIF1 is frequently downregulated or silenced by promoter methylation in the carcinogenesis of OSF, which serves as a potential epigenetic biomarker for the early detection of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou S, Chen L, Mashrah M, Zhu Y, He Z, Hu Y, Xiang T, Yao Z, Guo F, Zhang C, Zhang C, et al: Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis. Oncol Rep 34: 2636-2642, 2015.
APA
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y. ... Zhang, C. (2015). Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis. Oncology Reports, 34, 2636-2642. https://doi.org/10.3892/or.2015.4264
MLA
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y., Xiang, T., Yao, Z., Guo, F., Zhang, C."Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis". Oncology Reports 34.5 (2015): 2636-2642.
Chicago
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y., Xiang, T., Yao, Z., Guo, F., Zhang, C."Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis". Oncology Reports 34, no. 5 (2015): 2636-2642. https://doi.org/10.3892/or.2015.4264
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou S, Chen L, Mashrah M, Zhu Y, He Z, Hu Y, Xiang T, Yao Z, Guo F, Zhang C, Zhang C, et al: Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis. Oncol Rep 34: 2636-2642, 2015.
APA
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y. ... Zhang, C. (2015). Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis. Oncology Reports, 34, 2636-2642. https://doi.org/10.3892/or.2015.4264
MLA
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y., Xiang, T., Yao, Z., Guo, F., Zhang, C."Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis". Oncology Reports 34.5 (2015): 2636-2642.
Chicago
Zhou, S., Chen, L., Mashrah, M., Zhu, Y., He, Z., Hu, Y., Xiang, T., Yao, Z., Guo, F., Zhang, C."Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis". Oncology Reports 34, no. 5 (2015): 2636-2642. https://doi.org/10.3892/or.2015.4264
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