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Article

Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture

  • Authors:
    • Kewen Zheng
    • Yaling Dou
    • Linfu He
    • Hanzhong Li
    • Zhicai Zhang
    • Yu Chen
    • Ali Ye
    • Wenjing Liu
    • Lingjun Kong
  • View Affiliations / Copyright

    Affiliations: Urology Department, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, P.R. China, Laboratory Medicine Department, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, P.R. China, Institute of Bioengineering, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
  • Pages: 2439-2444
    |
    Published online on: September 9, 2015
       https://doi.org/10.3892/or.2015.4266
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Abstract

Prostate cancer antigen 3 (PCA3) is a non-coding RNA fragment that is overexpressed in prostate cancer cells. However, the clinical applications of PCA3 are highly limited due to the instability of RNA and the lack of reliable and efficient RNA extraction and purification methods. Thus, in the present study, we compared three different methods of RNA extraction to further confirm the higher yield of commercial magnetic beads with poly-T functionalization and a capturer strand. The current protocols for RNA extraction of i) the phenol-chloroform method, ii) the affinity column method and iii) magnetic beads with poly-T functionalization and a capturer strand were applied separately for RNA extraction in urine samples. Reverse transcription‑quantitative polymerase chain reaction was performed to evaluate the yield of the three methods of RNA extraction. Furthermore, 52 urine samples after prostate massage from patients suspected of a diagnosis of prostate cancer were collected. The Mag-Cap method and RT-PCR were applied to obtain the PCA3 score. The clinical value of the PCA3 score was investigated by comparison with the pathology of the prostate biopsy. The yield of the Mag-Cap method was higher than that of the phenol‑chloroform method and commercial kits. Thirty‑four patients were pathologically diagnosed with prostate cancer and 18 with benign prostatic hyperplasia (BPH). It was confirmed that the median PCA3 score was higher among the prostate cancer patients than those with benign disease (53.5 vs. 17, p=0.000). A sensitivity of 82.4% and a specificity of 77.8% were obtained when the cut-off value for the PCA3 score was 28.5. The Mag-Cap method was found to be more efficient for RNA extraction. The urinary PCA3 score is a promising method for prostate cancer screening, detection and diagnosis, and has the potential to reduce unnecessary prostate biopsies.
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1 

Siegel R, DeSantis C, Virgo K, Stein K, Mariotto A, Smith T, Cooper D, Gansler T, Lerro C, Fedewa S, et al: Cancer treatment and survivorship statistics, 2012. CA Cancer J Clin. 62:220–241. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Surveillance E; End Results (SEER) Program: (www.seer.cancer.gov) SEER*Stat Database: Mortality - All COD, Aggregated With State, Total U.S. (1969–2010) <Katrina/Rita Population Adjustment>, National Cancer Institute, DCCPS, Surveillance Research Program, Surveillance Systems Branch, released April 2013. Underlying mortality data provided by NCHS (www.cdc.gov/nchs)

4 

Chen W, Zheng R, Zhang S, Zhao P, Li G, Wu L and He J: The incidences and mortalities of major cancers in China, 2009. Chin J Cancer. 32:106–112. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Smith RA, Cokkinides V and Eyre HJ: Cancer screening in the United States, 2007: A review of current guidelines, practices, and prospects. CA Cancer J Clin. 57:90–104. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Slawin KM, Ohori M, Dillioglugil O and Scardino PT: Screening for prostate cancer: An analysis of the early experience. CA Cancer J Clin. 45:134–147. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Catalona WJ, Richie JP, Ahmann FR, Hudson MA, Scardino PT, Flanigan RC, deKernion JB, Ratliff TL, Kavoussi LR, Dalkin BL, et al: Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: Results of a multicenter clinical trial of 6,630 men. J Urol. 151:1283–1290. 1994.PubMed/NCBI

8 

Cher ML and Carroll PR: Screening for prostate cancer. West J Med. 162:235–242. 1995.PubMed/NCBI

9 

Schmidt JD: Clinical diagnosis of prostate cancer. Cancer. 70(Suppl 1): 221–224. 1992. View Article : Google Scholar : PubMed/NCBI

10 

Lilja H: Biology of prostate-specific antigen. Urology. 62(Suppl 1): 27–33. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Yates DR and Catto JW: Distinct patterns and behaviour of urothelial carcinoma with respect to anatomical location: How molecular biomarkers can augment clinicopathological predictors in upper urinary tract tumours. World J Urol. 31:21–29. 2013. View Article : Google Scholar

12 

Clarke RA, Zhao Z, Guo AY, Roper K, Teng L, Fang ZM, Samaratunga H, Lavin MF and Gardiner RA: New genomic structure for prostate cancer specific gene PCA3 within BMCC1: Implications for prostate cancer detection and progression. PLoS One. 4:e49952009. View Article : Google Scholar : PubMed/NCBI

13 

Roddam AW, Duffy MJ, Hamdy FC, Ward AM, Patnick J, Price CP, Rimmer J, Sturgeon C, White P and Allen NE; NHS Prostate Cancer Risk Management: Use of prostate-specific antigen (PSA) isoforms for the detection of prostate cancer in men with a PSA level of 2–10 ng/ml: Systematic review and meta-analysis. Eur Urol. 48:386–399. 2005. View Article : Google Scholar

14 

Bussemakers MJ, van Bokhoven A, Verhaegh GW, Smit FP, Karthaus HF, Schalken JA, Debruyne FM, Ru N and Isaacs WB: DD3: A new prostate-specific gene, highly overexpressed in prostate cancer. Cancer Res. 59:5975–5979. 1999.PubMed/NCBI

15 

Hessels D, Klein Gunnewiek JM, van Oort I, Karthaus HF, van Leenders GJ, van Balken B, Kiemeney LA, Witjes JA and Schalken JA: DD3(PCA3)-based molecular urine analysis for the diagnosis of prostate cancer. Eur Urol. 44:8–16. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Schalken JA, Hessels D and Verhaegh G: New targets for therapy in prostate cancer: Differential display code 3 (DD3(PCA3)), a highly prostate cancer-specific gene. Urology. 62(Suppl 1): 34–43. 2003. View Article : Google Scholar : PubMed/NCBI

17 

de Kok JB, Verhaegh GW, Roelofs RW, Hessels D, Kiemeney LA, Aalders TW, Swinkels DW and Schalken JA: DD3(PCA3), a very sensitive and specific marker to detect prostate tumors. Cancer Res. 62:2695–2698. 2002.PubMed/NCBI

18 

Day JR, Jost M, Reynolds MA, Groskopf J and Rittenhouse H: PCA3: From basic molecular science to the clinical lab. Cancer Lett. 301:1–6. 2011. View Article : Google Scholar

19 

Ploussard G and de la Taille A: Urine biomarkers in prostate cancer. Nat Rev Urol. 7:101–109. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Roobol MJ: Contemporary role of prostate cancer gene 3 in the management of prostate cancer. Curr Opin Urol. 21:225–229. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Roobol MJ, Haese A and Bjartell A: Tumour markers in prostate cancer III: Biomarkers in urine. Acta Oncol. 50(Suppl 1): 85–89. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Marks LS, Fradet Y, Deras IL, Blase A, Mathis J, Aubin SM, Cancio AT, Desaulniers M, Ellis WJ, Rittenhouse H, et al: PCA3 molecular urine assay for prostate cancer in men undergoing repeat biopsy. Urology. 69:532–535. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Janegitz BC, Cancino J and Zucolotto V: Disposable biosensors for clinical diagnosis. J Nanosci Nanotechnol. 14:378–389. 2014. View Article : Google Scholar : PubMed/NCBI

24 

McCarroll J, Teo J, Boyer C, Goldstein D, Kavallaris M and Phillips PA: Potential applications of nanotechnology for the diagnosis and treatment of pancreatic cancer. Front Physiol. 5:22014. View Article : Google Scholar : PubMed/NCBI

25 

Thakor AS and Gambhir SS: Nanooncology: The future of cancer diagnosis and therapy. CA Cancer J Clin. 63:395–418. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Chan CP, Cheung YC, Renneberg R and Seydack M: New trends in immunoassays. Adv Biochem Eng Biotechnol. 109:123–154. 2008.

27 

Sandhu A, Handa H and Abe M: Synthesis and applications of magnetic nanoparticles for biorecognition and point of care medical diagnostics. Nanotechnology. 21:4420012010. View Article : Google Scholar : PubMed/NCBI

28 

Casadio V, Calistri D, Salvi S, Gunelli R, Carretta E, Amadori D, Silvestrini R and Zoli W: Urine cell-free DNA integrity as a marker for early prostate cancer diagnosis: A pilot study. Biomed Res Int. 2013:2704572013. View Article : Google Scholar

29 

Bettegowda C, Sausen M, Leary RJ, Kinde I, Wang Y, Agrawal N, Bartlett BR, Wang H, Luber B, Alani RM, et al: Detection of circulating tumor DNA in early- and late-stage human malignancies. Sci Transl Med. 6:224ra242014. View Article : Google Scholar : PubMed/NCBI

30 

Ahyai SA, Graefen M, Steuber T, Haese A, Schlomm T, Walz J, Köllermann J, Briganti A, Zacharias M, Friedrich MG, et al: Contemporary prostate cancer prevalence among T1c biopsy-referred men with a prostate-specific antigen level ≤4.0 ng per milliliter. Eur Urol. 53:750–757. 2008. View Article : Google Scholar

31 

Thompson IM, Pauler DK, Goodman PJ, Tangen CM, Lucia MS, Parnes HL, Minasian LM, Ford LG, Lippman SM, Crawford ED, et al: Prevalence of prostate cancer among men with a prostate-specific antigen level ≤4.0 ng per milliliter. N Engl J Med. 350:2239–2246. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Groskopf J, Aubin SM, Deras IL, Blase A, Bodrug S, Clark C, Brentano S, Mathis J, Pham J, Meyer T, et al: APTIMA PCA3 molecular urine test: development of a method to aid in the diagnosis of prostate cancer. Clin Chem. 52:1089–1095. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zheng K, Dou Y, He L, Li H, Zhang Z, Chen Y, Ye A, Liu W and Kong L: Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture. Oncol Rep 34: 2439-2444, 2015.
APA
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y. ... Kong, L. (2015). Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture. Oncology Reports, 34, 2439-2444. https://doi.org/10.3892/or.2015.4266
MLA
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y., Ye, A., Liu, W., Kong, L."Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture". Oncology Reports 34.5 (2015): 2439-2444.
Chicago
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y., Ye, A., Liu, W., Kong, L."Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture". Oncology Reports 34, no. 5 (2015): 2439-2444. https://doi.org/10.3892/or.2015.4266
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng K, Dou Y, He L, Li H, Zhang Z, Chen Y, Ye A, Liu W and Kong L: Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture. Oncol Rep 34: 2439-2444, 2015.
APA
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y. ... Kong, L. (2015). Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture. Oncology Reports, 34, 2439-2444. https://doi.org/10.3892/or.2015.4266
MLA
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y., Ye, A., Liu, W., Kong, L."Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture". Oncology Reports 34.5 (2015): 2439-2444.
Chicago
Zheng, K., Dou, Y., He, L., Li, H., Zhang, Z., Chen, Y., Ye, A., Liu, W., Kong, L."Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture". Oncology Reports 34, no. 5 (2015): 2439-2444. https://doi.org/10.3892/or.2015.4266
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