Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of transcription-3 signaling pathway

  • Authors:
    • Enjiao Zhang
    • Zhenning Li
    • Zhongfei Xu
    • Weiyi Duan
    • Changfu Sun
    • Li Lu
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  • Published online on: September 17, 2015     https://doi.org/10.3892/or.2015.4285
  • Pages: 3061-3067
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Abstract

Frizzled2 (Fzd2) is a receptor for wingless-type MMTV integration site family members (Wnts), the aberrant overexpression of which has been noted to contribute to cancer metastasis. The present study was performed to characterize the role of Fzd2 in the migration and invasion of oral squamous cell carcinomas (OSCC) in vitro. Using TSCCa cells (a tongue SCC cell line) for loss- or gain-of-function of Fzd2, we found that a forced overexpression of Fzd2 promoted TSCCa cell migration and invasion, decreased the expression of epithelial‑cadherin (E-cadherin, an epithelial marker) and increased that of vimentin, Snail Slug, matrix metalloproteinases (MMPs)-2/-9/-13 and a-disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS5). By contrast, RNA interference (RNAi)-mediated knockdown of Fzd2 had opposite effects on OSCC cells. In addition, we found that the phosphorylation of signal transducer and activator of transcription-3 (STAT3) was enhanced by Fzd2 overexpression, but suppressed by Fzd2 depletion, and that STAT3‑specific shRNA attenuated Fzd2 overexpression‑induced cell invasion. In summary, the present study demonstrated that Fzd2 contributes to the migration and invasion of OSCC cells, at least partly through regulation of the STAT3 pathway. These results suggest Fzd2 as a novel therapeutic target for OSCC.
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December-2015
Volume 34 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang E, Li Z, Xu Z, Duan W, Sun C and Lu L: Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of transcription-3 signaling pathway. Oncol Rep 34: 3061-3067, 2015
APA
Zhang, E., Li, Z., Xu, Z., Duan, W., Sun, C., & Lu, L. (2015). Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of transcription-3 signaling pathway. Oncology Reports, 34, 3061-3067. https://doi.org/10.3892/or.2015.4285
MLA
Zhang, E., Li, Z., Xu, Z., Duan, W., Sun, C., Lu, L."Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of transcription-3 signaling pathway". Oncology Reports 34.6 (2015): 3061-3067.
Chicago
Zhang, E., Li, Z., Xu, Z., Duan, W., Sun, C., Lu, L."Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of transcription-3 signaling pathway". Oncology Reports 34, no. 6 (2015): 3061-3067. https://doi.org/10.3892/or.2015.4285