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Article

Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells

  • Authors:
    • Young Shin Ko
    • Won Sup Lee
    • Young Nak Joo
    • Yung Hyun Choi
    • Gon Sup Kim
    • Jin-Myung Jung
    • Chung Ho Ryu
    • Sung Chul Shin
    • Hye Jung Kim
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 660-702, Republic of Korea, Department of Internal Medicine, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 660-702, Republic of Korea, Department of Biochemistry, College of Oriental Medicine, Dongeui University and Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Busan 614-052, Republic of Korea, School of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea, Department of Neurosurgery, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 660-702, Republic of Korea, Division of Applied Life Science (BK21 Program), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea, Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • Pages: 3035-3042
    |
    Published online on: September 22, 2015
       https://doi.org/10.3892/or.2015.4304
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Abstract

The Korean prostrate spurge Euphorbia supina is abundant in polyphenols and has been used as a folk medicine in Korea against a variety of diseases. Thus, we aimed to investigate the effect of polyphenol mixtures of Korean Euphorbia supina (PES) on the invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Firstly, PES showed no cytotoxicity on cancer cells and endothelial cells (ECs) at the doses of 0.1-10 µg/ml, but showed significant cytotoxicity from 50 µg/ml. Thus, we performed subsequent experiments with PES at doses up to 5 µg/ml. PES dose‑dependently suppressed epithelial-mesenchymal transition by downregulating the mesenchymal markers, Snail1 and N-cadherin, showing significant inhibition from 1 and 5 µg/ml, respectively. In addition, PES significantly inhibited MMP-9 activity and LOX release induced by TNF-α at 5 µg/ml. Then, we determined the effect of PES on the expression of adhesion molecules and VE-cadherin phosphorylation. The results showed that PES effectively reduced TNF-α-mediated VCAM-1 expression but not ICAM expression both in the MDA-MB-231 cells and ECs, resulting in the reduced adhesion of MDA-MB-231 to ECs. Finally, PES effectively inhibited MDA-MB-231 cell invasion through ECs, suggesting that PES may serve as a therapeutic agent against cancer metastasis with minimal cytotoxicity to normal cells.
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Copy and paste a formatted citation
Spandidos Publications style
Ko YS, Lee WS, Joo YN, Choi YH, Kim GS, Jung J, Ryu CH, Shin SC and Kim HJ: Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Oncol Rep 34: 3035-3042, 2015.
APA
Ko, Y.S., Lee, W.S., Joo, Y.N., Choi, Y.H., Kim, G.S., Jung, J. ... Kim, H.J. (2015). Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Oncology Reports, 34, 3035-3042. https://doi.org/10.3892/or.2015.4304
MLA
Ko, Y. S., Lee, W. S., Joo, Y. N., Choi, Y. H., Kim, G. S., Jung, J., Ryu, C. H., Shin, S. C., Kim, H. J."Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells". Oncology Reports 34.6 (2015): 3035-3042.
Chicago
Ko, Y. S., Lee, W. S., Joo, Y. N., Choi, Y. H., Kim, G. S., Jung, J., Ryu, C. H., Shin, S. C., Kim, H. J."Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells". Oncology Reports 34, no. 6 (2015): 3035-3042. https://doi.org/10.3892/or.2015.4304
Copy and paste a formatted citation
x
Spandidos Publications style
Ko YS, Lee WS, Joo YN, Choi YH, Kim GS, Jung J, Ryu CH, Shin SC and Kim HJ: Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Oncol Rep 34: 3035-3042, 2015.
APA
Ko, Y.S., Lee, W.S., Joo, Y.N., Choi, Y.H., Kim, G.S., Jung, J. ... Kim, H.J. (2015). Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Oncology Reports, 34, 3035-3042. https://doi.org/10.3892/or.2015.4304
MLA
Ko, Y. S., Lee, W. S., Joo, Y. N., Choi, Y. H., Kim, G. S., Jung, J., Ryu, C. H., Shin, S. C., Kim, H. J."Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells". Oncology Reports 34.6 (2015): 3035-3042.
Chicago
Ko, Y. S., Lee, W. S., Joo, Y. N., Choi, Y. H., Kim, G. S., Jung, J., Ryu, C. H., Shin, S. C., Kim, H. J."Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells". Oncology Reports 34, no. 6 (2015): 3035-3042. https://doi.org/10.3892/or.2015.4304
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