β-asarone inhibits gastric cancer cell proliferation

  • Authors:
    • Jian Wu
    • Xing-Xing Zhang
    • Qing-Min Sun
    • Min Chen
    • Sheng‑Lin Liu
    • Xiang Zhang
    • Jin-Yong Zhou
    • Xi Zou
  • View Affiliations

  • Published online on: September 30, 2015     https://doi.org/10.3892/or.2015.4316
  • Pages: 3043-3050
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Abstract

β-asarone is the main volatile oil of Chinese herb Rhizoma Acori Tatarinowii. It exhibits a wide range of biological activities in many human organs. However, few studies have investigated the effect of β-asarone on gastric cancer. The present study investigated the effect of β-asarone on the proliferation and apoptosis of three types of differentiated human gastric cancer cell lines (SGC-7901, BGC-823 and MKN-28) in vitro as well as the related molecular mechanisms. Methyl thiazolyl tetrazolium assay, Annexin V/PI double staining, immunofluorescence test and transmission electron microscopy all confirmed that β-asarone had an obvious dose-dependent inhibitive effect on the proliferation of human gastric cancer cells and induced apoptosis of the cell lines. Transwell invasion, wound-healing and matrix‑cell adhesion experiments confirmed that β-asarone inhibited the invasion, migration and adhesion of human gastric cancer BGC-823 cells. Quantitative real-time PCR and western blotting found that β-asarone significantly activated caspase-3, caspase-8, caspase-9, Bax, Bak and suppressed Bcl-2, Bcl-xL and survivin activity. Moreover, β-asarone increased the expression of RECK, E-cadherin and decreased the expression of MMP-2, MMP-9, MMP-14 and N-cadherin. The present study demonstrated that β-asarone effectively inhibits the proliferation of human gastric cancer cells, induces their apoptosis and decreased the invasive, migratory and adhesive abilities.
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December-2015
Volume 34 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu J, Zhang X, Sun Q, Chen M, Liu SL, Zhang X, Zhou J and Zou X: β-asarone inhibits gastric cancer cell proliferation. Oncol Rep 34: 3043-3050, 2015
APA
Wu, J., Zhang, X., Sun, Q., Chen, M., Liu, S., Zhang, X. ... Zou, X. (2015). β-asarone inhibits gastric cancer cell proliferation. Oncology Reports, 34, 3043-3050. https://doi.org/10.3892/or.2015.4316
MLA
Wu, J., Zhang, X., Sun, Q., Chen, M., Liu, S., Zhang, X., Zhou, J., Zou, X."β-asarone inhibits gastric cancer cell proliferation". Oncology Reports 34.6 (2015): 3043-3050.
Chicago
Wu, J., Zhang, X., Sun, Q., Chen, M., Liu, S., Zhang, X., Zhou, J., Zou, X."β-asarone inhibits gastric cancer cell proliferation". Oncology Reports 34, no. 6 (2015): 3043-3050. https://doi.org/10.3892/or.2015.4316