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Article

miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor

Retraction in: /10.3892/or.2021.8113
  • Authors:
    • Xiaoli Pang
    • Kexin Huang
    • Qianqian Zhang
    • Yujiao Zhang
    • Junqi Niu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Gastroenterology, The First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, The Experiment Center, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Hepatology, The First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 3272-3279
    |
    Published online on: October 1, 2015
       https://doi.org/10.3892/or.2015.4321
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Abstract

MicroRNA-154 (miR-154) has been identified as a tumor suppressor in several types of human cancers; however, its clinical significance and function in human hepatocellular carcinoma (HCC) remain unclear. The aim of the present study was to analyze the clinical significance and cellular function of miR-154 in HCC patients. The data showed that miR-154 expression was consistently lower in HCC tissues and cell lines compared to that in matched tumor-adjacent tissues and a normal hepatic cell line, and its expression was negatively correlated with tumor differentiation (P<0.01), TNM stage (P<0.01) and lymph node metastasis (P<0.01). Restoration of miR-154 expression in HepG2 cells inhibited cell proliferation, migration and invasion, and induced apoptosis and cell arrest at the G1 phase in vitro, as well as suppressed tumor growth in a nude mouse model. Using a luciferase assay, we identified that miR-154 was able to target the 3'-untranslated region (3'UTR) of ZEB2 mRNA. Then, we revealed that miR-154 was able to reduce ZEB2 expression at the levels of mRNA and protein using qRT-PCR and western blot analysis. Notably, restoration of expression of ZEB2 weakened miR-154-mediated suppression of tumor progression. In conclusion, these results indicate that miR-154 functions as a tumor suppressor in HCC by suppressing ZEB2, suggesting that miR-154 may serve as a potential target for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Pang X, Huang K, Zhang Q, Zhang Y and Niu J: miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113. Oncol Rep 34: 3272-3279, 2015.
APA
Pang, X., Huang, K., Zhang, Q., Zhang, Y., & Niu, J. (2015). miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113. Oncology Reports, 34, 3272-3279. https://doi.org/10.3892/or.2015.4321
MLA
Pang, X., Huang, K., Zhang, Q., Zhang, Y., Niu, J."miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113". Oncology Reports 34.6 (2015): 3272-3279.
Chicago
Pang, X., Huang, K., Zhang, Q., Zhang, Y., Niu, J."miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113". Oncology Reports 34, no. 6 (2015): 3272-3279. https://doi.org/10.3892/or.2015.4321
Copy and paste a formatted citation
x
Spandidos Publications style
Pang X, Huang K, Zhang Q, Zhang Y and Niu J: miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113. Oncol Rep 34: 3272-3279, 2015.
APA
Pang, X., Huang, K., Zhang, Q., Zhang, Y., & Niu, J. (2015). miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113. Oncology Reports, 34, 3272-3279. https://doi.org/10.3892/or.2015.4321
MLA
Pang, X., Huang, K., Zhang, Q., Zhang, Y., Niu, J."miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113". Oncology Reports 34.6 (2015): 3272-3279.
Chicago
Pang, X., Huang, K., Zhang, Q., Zhang, Y., Niu, J."miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor Retraction in /10.3892/or.2021.8113". Oncology Reports 34, no. 6 (2015): 3272-3279. https://doi.org/10.3892/or.2015.4321
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