Differences in urinary proteins related to surgical margin status after radical prostatectomy

  • Authors:
    • Zbynek Heger
    • Petr Michalek
    • Roman Guran
    • Natalia Cernei
    • Katerina Duskova
    • Stepan Vesely
    • Jiri Anyz
    • Olga Stepankova
    • Ondrej Zitka
    • Vojtech Adam
    • Rene Kizek
  • View Affiliations

  • Published online on: October 2, 2015     https://doi.org/10.3892/or.2015.4322
  • Pages: 3247-3255
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Abstract

Presented exploratory pilot study was aimed at evaluation of proteins present in urinary specimens collected from prostate cancer suffering subjects after radical prostatectomy, divided into two experimental cohorts: positive (n=15) and negative (n=15) surgical margins (PSM/NSM). The presence of PSM suggests inadequate cancer clearance and the possible need for additional treatment. Proper identification of these risk-patients is therefore of a paramount importance. Total protein profiles were firstly identified by using SDS-PAGE and compared by using partial least square discrimination analysis (PLS-DA), which revealed differences in molecular weights of 80-99 and 150-235 kDa between the experimental groups. For further identification of proteins, comparative proteomic technologies were employed. Two-dimensional gel electrophoresis with subsequent identification of protein spots by using MALDI-TOF mass fingerprinting revealed differential expression of proteins between NSM/PSM cohorts. Moreover, in PSM group, three uniquely identified proteins (cyclin-dependent kinase 6, galectin-3-binding protein and L-lactate dehydrogenase C chain) were found, which show tight connection with prostate cancer and presence of all of them was previously linked to certain aspects of prostate cancer. These proteins may be associated with the molecular mechanisms of prostate cancer development; hence, their identification may be helpful for the assessment of disease progression risk after radical prostatectomy, but also for possible early diagnosis.
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December-2015
Volume 34 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Heger Z, Michalek P, Guran R, Cernei N, Duskova K, Vesely S, Anyz J, Stepankova O, Zitka O, Adam V, Adam V, et al: Differences in urinary proteins related to surgical margin status after radical prostatectomy. Oncol Rep 34: 3247-3255, 2015
APA
Heger, Z., Michalek, P., Guran, R., Cernei, N., Duskova, K., Vesely, S. ... Kizek, R. (2015). Differences in urinary proteins related to surgical margin status after radical prostatectomy. Oncology Reports, 34, 3247-3255. https://doi.org/10.3892/or.2015.4322
MLA
Heger, Z., Michalek, P., Guran, R., Cernei, N., Duskova, K., Vesely, S., Anyz, J., Stepankova, O., Zitka, O., Adam, V., Kizek, R."Differences in urinary proteins related to surgical margin status after radical prostatectomy". Oncology Reports 34.6 (2015): 3247-3255.
Chicago
Heger, Z., Michalek, P., Guran, R., Cernei, N., Duskova, K., Vesely, S., Anyz, J., Stepankova, O., Zitka, O., Adam, V., Kizek, R."Differences in urinary proteins related to surgical margin status after radical prostatectomy". Oncology Reports 34, no. 6 (2015): 3247-3255. https://doi.org/10.3892/or.2015.4322