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Article

MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma

Retraction in: /10.3892/or.2025.9019
  • Authors:
    • Zhiming Liu
    • Cynthia Diep
    • Tiantian Mao
    • Li Huang
    • Robert Merrill
    • Zhouwen Zhang
    • Youjian Peng
  • View Affiliations / Copyright

    Affiliations: Stomatology Center, Renmin Hospital of Wuhan University, Wuchang, Wuhan, Hubei 430060, P.R. China, UCLA School of Dentistry, Los Angeles, CA 951668, USA
  • Pages: 2961-2968
    |
    Published online on: October 2, 2015
       https://doi.org/10.3892/or.2015.4323
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Abstract

miR-92b has been reported to be dysregulated in many types of human cancers. However, the role of miR-92b in oral squamous cell carcinoma (OSCC) is unknown. The aim of the present study was to investigate the function and mechanism of miR-92b in human OSCC. Using quantitative reverse‑transcription PCR (qRT-PCR), we found that the miR-92b level in primary tumors (n=85) was significantly elevated compared with that in the adjacent normal tissues (p<0.001). A high level of miR-92b was significantly associated with a large tumor size (p=0.005), advanced tumor stage (p<0.001) and poorer prognosis (p=0.04). Functionally, miR-92b was shown to not only promote the proliferation of OSCC cells in MTT and colony formation and xenograft assays, but also to inhibit cell apoptosis in a flow cytometric assay. In western blotting and luciferase assay, NLK was identified as a direct and functional target of miR-92b. We also found that NLK was involved in miR-92b-induced cell proliferation, and its protein level was obviously downregulated in the miR-92b-overexpressing xenograft tumors. Finally, luciferase reporter assay and fluorescent immunostaining revealed that miR-92b activated the NF-κB signaling pathway, which may be responsible for the effects of miR-92b on cell proliferation. Taken together, our results indicate that miR-92b upregulation accelerates tumor growth and present a novel mechanism of miRNA‑mediated NF-κB activation in OSCC.
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1 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Schickel R, Boyerinas B, Park SM and Peter ME: MicroRNAs: Key players in the immune system, differentiation, tumorigenesis and cell death. Oncogene. 27:5959–5974. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Chen F and Hu SJ: Effect of microRNA-34a in cell cycle, differentiation, and apoptosis: A review. J Biochem Mol Toxicol. 26:79–86. 2012. View Article : Google Scholar

4 

Worsham MJ, Ali H, Dragovic J and Schweitzer VP: Molecular characterization of head and neck cancer: How close to personalized targeted therapy? Mol Diagn Ther. 16:209–222. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Leemans CR, Braakhuis BJ and Brakenhoff RH: The molecular biology of head and neck cancer. Nat Rev Cancer. 11:9–22. 2011. View Article : Google Scholar

6 

Liu CJ, Tsai MM, Hung PS, Kao SY, Liu TY, Wu KJ, Chiou SH, Lin SC and Chang KW: miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma. Cancer Res. 70:1635–1644. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Kinoshita T, Hanazawa T, Nohata N, Kikkawa N, Enokida H, Yoshino H, Yamasaki T, Hidaka H, Nakagawa M, Okamoto Y, et al: Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion through targeting laminin-332 in head and neck squamous cell carcinoma. Oncotarget. 3:1386–1400. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Wu X, Bhayani MK, Dodge CT, Nicoloso MS, Chen Y, Yan X, Adachi M, Thomas L, Galer CE, Jiffar T, et al: Coordinated targeting of the EGFR signaling axis by microRNA-27a*. Oncotarget. 4:1388–1398. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Wu ZB, Cai L, Lin SJ, Lu JL, Yao Y and Zhou LF: The miR-92b functions as a potential oncogene by targeting on Smad3 in glioblastomas. Brain Res. 1529:16–25. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li Q, Shen K, Zhao Y, Ma C, Liu J and Ma J: MiR-92b inhibitor promoted glioma cell apoptosis via targeting DKK3 and blocking the Wnt/beta-catenin signaling pathway. J Transl Med. 11:3022013. View Article : Google Scholar : PubMed/NCBI

11 

Shah AA, Leidinger P, Blin N and Meese E: miRNA: Small molecules as potential novel biomarkers in cancer. Curr Med Chem. 17:4427–4432. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Meng F, Glaser SS, Francis H, DeMorrow S, Han Y, Passarini JD, Stokes A, Cleary JP, Liu X, Venter J, et al: Functional analysis of microRNAs in human hepatocellular cancer stem cells. J Cell Mol Med. 16:160–173. 2012. View Article : Google Scholar

13 

Nass D, Rosenwald S, Meiri E, Gilad S, Tabibian-Keissar H, Schlosberg A, Kuker H, Sion-Vardy N, Tobar A, Kharenko O, et al: MiR-92b and miR-9/9* are specifically expressed in brain primary tumors and can be used to differentiate primary from metastatic brain tumors. Brain Pathol. 19:375–383. 2009. View Article : Google Scholar :

14 

Haug BH, Henriksen JR, Buechner J, Geerts D, Tømte E, Kogner P, Martinsson T, Flægstad T, Sveinbjørnsson B and Einvik C: MYCN-regulated miRNA-92 inhibits secretion of the tumor suppressor DICKKOPF-3 (DKK3) in neuroblastoma. Carcinogenesis. 32:1005–1012. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Li Y, Li L, Guan Y, Liu X, Meng Q and Guo Q: MiR-92b regulates the cell growth, cisplatin chemosensitivity of A549 non small cell lung cancer cell line and target PTEN. Biochem Biophys Res Commun. 440:604–610. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Wang K, Wang X, Zou J, Zhang A, Wan Y, Pu P, Song Z, Qian C, Chen Y, Yang S, et al: miR-92b controls glioma proliferation and invasion through regulating Wnt/beta-catenin signaling via Nemo-like kinase. Neuro Oncol. 15:578–588. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Allen CT, Ricker JL, Chen Z and Van Waes C: Role of activated nuclear factor-kappaB in the pathogenesis and therapy of squamous cell carcinoma of the head and neck. Head Neck. 29:959–971. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Bhave SL, Teknos TN and Pan Q: Molecular parameters of head and neck cancer metastasis. Crit Rev Eukaryot Gene Expr. 21:143–153. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Lu Z and Li Y, Takwi A, Li B, Zhang J, Conklin DJ, Young KH, Martin R and Li Y: miR-301a as an NF-κB activator in pancreatic cancer cells. EMBO J. 30:57–67. 2011. View Article : Google Scholar :

20 

Jiang L, Lin C, Song L, Wu J, Chen B, Ying Z, Fang L, Yan X, He M, Li J, et al: MicroRNA-30e* promotes human glioma cell invasiveness in an orthotopic xenotransplantation model by disrupting the NF-κB/IκBα negative feedback loop. J Clin Invest. 122:33–47. 2012. View Article : Google Scholar :

21 

Ding J, Huang S, Wang Y, Tian Q, Zha R, Shi H, Wang Q, Ge C, Chen T, Zhao Y, et al: Genome-wide screening reveals that miR-195 targets the TNF-α/NF-κB pathway by down-regulating IκB kinase alpha and TAB3 in hepatocellular carcinoma. Hepatology. 58:654–666. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Katoh M and Katoh M: Transcriptional mechanisms of WNT5A based on NF-kappaB, Hedgehog, TGFbeta, and Notch signaling cascades. Int J Mol Med. 23:763–769. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Yasuda J, Yokoo H, Yamada T, Kitabayashi I, Sekiya T and Ichikawa H: Nemo-like kinase suppresses a wide range of transcription factors, including nuclear factor-kappaB. Cancer Sci. 95:52–57. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Li SZ, Zhang HH, Liang JB, Song Y, Jin BX, Xing NN, Fan GC, Du RL and Zhang XD: Nemo-like kinase (NLK) negatively regulates NF-kappa B activity through disrupting the interaction of TAK1 with IKKβ. Biochim Biophys Acta. 1843:1365–1372. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Diep C, Mao T, Huang L, Merrill R, Zhang Z and Peng Y: MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019. Oncol Rep 34: 2961-2968, 2015.
APA
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., & Peng, Y. (2015). MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019. Oncology Reports, 34, 2961-2968. https://doi.org/10.3892/or.2015.4323
MLA
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., Peng, Y."MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019". Oncology Reports 34.6 (2015): 2961-2968.
Chicago
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., Peng, Y."MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019". Oncology Reports 34, no. 6 (2015): 2961-2968. https://doi.org/10.3892/or.2015.4323
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Diep C, Mao T, Huang L, Merrill R, Zhang Z and Peng Y: MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019. Oncol Rep 34: 2961-2968, 2015.
APA
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., & Peng, Y. (2015). MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019. Oncology Reports, 34, 2961-2968. https://doi.org/10.3892/or.2015.4323
MLA
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., Peng, Y."MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019". Oncology Reports 34.6 (2015): 2961-2968.
Chicago
Liu, Z., Diep, C., Mao, T., Huang, L., Merrill, R., Zhang, Z., Peng, Y."MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma Retraction in /10.3892/or.2025.9019". Oncology Reports 34, no. 6 (2015): 2961-2968. https://doi.org/10.3892/or.2015.4323
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