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Article

MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma

Retraction in: /10.3892/or.2021.8194
  • Authors:
    • Shoulin Zhang
    • Dongmei Zhang
    • Chunguang Yi
    • Yinping Wang
    • Hongan Wang
    • Jian Wang
  • View Affiliations / Copyright

    Affiliations: Internal Medicine Department, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin 130021, P.R. China, Scientific Research Office, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin 130021, P.R. China, College of Pharmacy, Changchun University of Chinese Medicine, Changchun Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117, P.R. China
  • Pages: 559-567
    |
    Published online on: October 16, 2015
       https://doi.org/10.3892/or.2015.4333
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Abstract

Accumulating evidence demonstrates that microRNA-22 (miR-22) was deregulated in many types of cancers and was involved in various cellular processes related to carcinogenesis. However, the exact roles and mechanisms of miR-22 remain unknown in human renal cell carcinoma (RCC). Here, the relationship between miR-22 expression pattern and clinicopathological features of patients with EOC were determined by real-time quantitative RT-PCR (qRT-PCR). Furthermore, the role of miR-22 and possible molecular mechanisms in EOC were investigated by several in vitro approaches and in a nude mouse model. Results from qRT-PCR showed that miR-22 was significantly downregulated in RCC samples compared with corresponding non-cancerous tissues, which was significantly associated with tumor stage and lymph node metastasis. Functional study demonstrated that enforced overexpression of miR-22 in renal cancer cells inhibited proliferation, migration and invasion, and induced cell apoptosis in vitro, and suppressed tumor growth in vivo. In addition, SIRT1 was identified as a direct target of miR-22 by a luciferase reporter assay. Overexpression of miR-22 activated p53 and its downstream target p21 and PUMA, and the apoptosis markers cleaved CASP3 and PARP, and inhibited epithelial-mesenchymal transition (EMT). These findings showed that miR-22 functioned as tumor suppressor in RCC and blocked RCC growth and metastasis by directly targeting SIRT1 in RCC, indicating a potential novel therapeutic role in RCC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Zhang D, Yi C, Wang Y, Wang H and Wang J: MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194. Oncol Rep 35: 559-567, 2016.
APA
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., & Wang, J. (2016). MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194. Oncology Reports, 35, 559-567. https://doi.org/10.3892/or.2015.4333
MLA
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., Wang, J."MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194". Oncology Reports 35.1 (2016): 559-567.
Chicago
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., Wang, J."MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194". Oncology Reports 35, no. 1 (2016): 559-567. https://doi.org/10.3892/or.2015.4333
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Zhang D, Yi C, Wang Y, Wang H and Wang J: MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194. Oncol Rep 35: 559-567, 2016.
APA
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., & Wang, J. (2016). MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194. Oncology Reports, 35, 559-567. https://doi.org/10.3892/or.2015.4333
MLA
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., Wang, J."MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194". Oncology Reports 35.1 (2016): 559-567.
Chicago
Zhang, S., Zhang, D., Yi, C., Wang, Y., Wang, H., Wang, J."MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma Retraction in /10.3892/or.2021.8194". Oncology Reports 35, no. 1 (2016): 559-567. https://doi.org/10.3892/or.2015.4333
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