Impact of KITENIN on tumor angiogenesis and lymphangiogenesis in colorectal cancer

  • Authors:
    • Hyung-Hoon Oh
    • Kang-Jin Park
    • Nuri Kim
    • Sun-Young Park
    • Young‑Lan Park
    • Chan-Young Oak
    • Dae-Seong Myung
    • Sung-Bum Cho
    • Wan‑Sik Lee
    • Kyung-Keun Kim
    • Young-Eun Joo
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  • Published online on: October 20, 2015     https://doi.org/10.3892/or.2015.4337
  • Pages: 253-260
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Abstract

Angiogenesis and lymphangiogenesis are involved in the dissemination of tumor cells from solid tumors to regional lymph nodes and various distant sites. KAI1 COOH-terminal interacting tetraspanin (KITENIN) contributes to tumor progression and poor clinical outcomes in various cancers including colorectal cancer. The aim of the present study was to evaluate whether KITENIN affects tumor angiogenesis and lymphangiogenesis in colorectal cancer. A KITENIN small interfering RNA vector was used to silence KITENIN expression in colorectal cancer cell lines including DLD1 and SW480 cells. To evaluate the ability of KITENIN to induce angiogenesis and lymphangiogenesis in human umbilical vein endothelial cells (HUVECs) and lymphatic endothelial cells (HLECs), we performed Matrigel invasion and tube formation assays. Immunohistochemistry was used to determine the expression of KITENIN in colorectal cancer tissues. Angiogenesis and lymphangiogenesis were evaluated by immunostaining with CD34 and D2-40 antibodies. KITENIN silencing inhibited both HUVEC invasion and tube formation in the DLD1 and SW480 cells. KITENIN silencing led to decreased expression of the angiogenic inducers vascular endothelial growth factor (VEGF)-A and hypoxia-inducible factor-1α and increased expression of the angiogenic inhibitor angiostatin. KITENIN silencing did not inhibit either HLEC invasion or tube formation in all tested cells, but it resulted in decreased expression of the lymphangiogenic inducer VEGF-C. KITENIN expression was significantly associated with tumor stage, depth of invasion, lymph node and distant metastases and poor survival. The mean microvessel density was significantly higher in the KITENIN-positive tumors than that in the KITENIN-negative tumors. However, the mean lymphatic vessel density of KITENIN-positive tumors was not significantly higher than that of the KITENIN-negative tumors. These results suggest that KITENIN promotes tumor progression by enhancing angiogenesis in colorectal cancer.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Oh H, Park K, Kim N, Park S, Park YL, Oak C, Myung D, Cho S, Lee WS, Kim K, Kim K, et al: Impact of KITENIN on tumor angiogenesis and lymphangiogenesis in colorectal cancer. Oncol Rep 35: 253-260, 2016
APA
Oh, H., Park, K., Kim, N., Park, S., Park, Y., Oak, C. ... Joo, Y. (2016). Impact of KITENIN on tumor angiogenesis and lymphangiogenesis in colorectal cancer. Oncology Reports, 35, 253-260. https://doi.org/10.3892/or.2015.4337
MLA
Oh, H., Park, K., Kim, N., Park, S., Park, Y., Oak, C., Myung, D., Cho, S., Lee, W., Kim, K., Joo, Y."Impact of KITENIN on tumor angiogenesis and lymphangiogenesis in colorectal cancer". Oncology Reports 35.1 (2016): 253-260.
Chicago
Oh, H., Park, K., Kim, N., Park, S., Park, Y., Oak, C., Myung, D., Cho, S., Lee, W., Kim, K., Joo, Y."Impact of KITENIN on tumor angiogenesis and lymphangiogenesis in colorectal cancer". Oncology Reports 35, no. 1 (2016): 253-260. https://doi.org/10.3892/or.2015.4337