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Article

Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells

  • Authors:
    • Zhiping He
    • Allen Y. Chen
    • Yon Rojanasakul
    • Gary O. Rankin
    • Yi Charlie Chen
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang A & F University, Lin'an, Zhejiang 311300, P.R. China, Department of Pharmaceutical Science, West Virginia University, Morgantown, WV 26506, USA, Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA
  • Pages: 291-297
    |
    Published online on: October 27, 2015
       https://doi.org/10.3892/or.2015.4354
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Abstract

Gallic acid (GA), a polyphenol, is widely found in numerous fruits and vegetables, particularly in hickory nuts. In the present study, we found that gallic acid, a natural phenolic compound isolated from fruits and vegetables, had a more potent growth inhibitory effect on two ovarian cancer cell lines, OVCAR-3 and A2780/CP70, than the effect on a normal ovarian cell line, IOSE-364. These results demonstrated that GA selectively inhibits the growth of cancer cells. Gene expression was examined by ELISA and western blot analysis, and gene pathways were examined by luciferase assay. It was found that GA inhibited VEGF secretion and suppressed in vitro angiogenesis in a concentration-dependent manner. GA downregulated AKT phosphorylation as well as HIF-1α expression but promoted PTEN expression. The luciferase assay results suggest that the PTEN/AKT/HIF-1α pathway accounts for the inhibitory effect of GA on VEGF expression and in vitro angiogenesis. These findings provide strong support for the high potential of GA in the prevention and therapy of ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
He Z, Chen AY, Rojanasakul Y, Rankin GO and Chen YC: Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells. Oncol Rep 35: 291-297, 2016.
APA
He, Z., Chen, A.Y., Rojanasakul, Y., Rankin, G.O., & Chen, Y.C. (2016). Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells. Oncology Reports, 35, 291-297. https://doi.org/10.3892/or.2015.4354
MLA
He, Z., Chen, A. Y., Rojanasakul, Y., Rankin, G. O., Chen, Y. C."Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells". Oncology Reports 35.1 (2016): 291-297.
Chicago
He, Z., Chen, A. Y., Rojanasakul, Y., Rankin, G. O., Chen, Y. C."Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells". Oncology Reports 35, no. 1 (2016): 291-297. https://doi.org/10.3892/or.2015.4354
Copy and paste a formatted citation
x
Spandidos Publications style
He Z, Chen AY, Rojanasakul Y, Rankin GO and Chen YC: Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells. Oncol Rep 35: 291-297, 2016.
APA
He, Z., Chen, A.Y., Rojanasakul, Y., Rankin, G.O., & Chen, Y.C. (2016). Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells. Oncology Reports, 35, 291-297. https://doi.org/10.3892/or.2015.4354
MLA
He, Z., Chen, A. Y., Rojanasakul, Y., Rankin, G. O., Chen, Y. C."Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells". Oncology Reports 35.1 (2016): 291-297.
Chicago
He, Z., Chen, A. Y., Rojanasakul, Y., Rankin, G. O., Chen, Y. C."Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells". Oncology Reports 35, no. 1 (2016): 291-297. https://doi.org/10.3892/or.2015.4354
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