Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo

  • Authors:
    • Lin Que
    • Dan Zhao
    • Xiu-Fa Tang
    • Ji-Yuan Liu
    • Xiang-Yu Zhang
    • Yu-Hua Zhan
    • Lei Zhang
  • View Affiliations

  • Published online on: November 2, 2015     https://doi.org/10.3892/or.2015.4374
  • Pages: 89-98
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Integrin-linked kinase (ILK), a highly conserved intracellular protein of serine/threonine protein kinase activities, which is associated with the integrin and growth factor receptor signaling pathway, is involved in the regulation of cell proliferation, apoptosis, differentiation, migration and epithelial-mesenchymal transition (EMT). Findings of a previous study showed that ILK overexpression was strongly correlated with a more aggressive tumor phenotype, recurrence and poor survival for oral squamous cell carcinoma (OSCC) patients, as well as some EMT markers. In order to investigate the underlying mechanisms involved, a lentivirus-mediated short hairpin RNA (shRNA) was employed to downregulate ILK. The results showed that the knockdown of ILK inhibited cell growth, adhesion and invasion ability in vitro, and OSCC cells deficient of ILK were blocked in the S phase and underwent apoptosis. Additionally, ILK shRNA inhibited EMT by impairing the expression of Snail, Slug and Twist2 and enhacning E-cadherin expression. ILK shRNA suppressed the phosphorylation of downstream signaling targets Akt and GSk-3β. In addition, the knockdown of ILK inhibited tumor growth, invasion and metastasis of xenograft tumors in vivo. These results suggested that ILK is a promising therapeutic target for the treatment of OSCC.
View Figures
View References

Related Articles

Journal Cover

January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Que L, Zhao D, Tang X, Liu J, Zhang X, Zhan Y and Zhang L: Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo. Oncol Rep 35: 89-98, 2016
APA
Que, L., Zhao, D., Tang, X., Liu, J., Zhang, X., Zhan, Y., & Zhang, L. (2016). Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo. Oncology Reports, 35, 89-98. https://doi.org/10.3892/or.2015.4374
MLA
Que, L., Zhao, D., Tang, X., Liu, J., Zhang, X., Zhan, Y., Zhang, L."Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo". Oncology Reports 35.1 (2016): 89-98.
Chicago
Que, L., Zhao, D., Tang, X., Liu, J., Zhang, X., Zhan, Y., Zhang, L."Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo". Oncology Reports 35, no. 1 (2016): 89-98. https://doi.org/10.3892/or.2015.4374