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Article

Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability

  • Authors:
    • Hyun-Woo Kim
    • Soo -A Kim
    • Sang-Gun Ahn
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea, Department of Biochemistry, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea
  • Pages: 235-242
    |
    Published online on: November 2, 2015
       https://doi.org/10.3892/or.2015.4381
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Abstract

The histone deacetylases (HDACs), Sirtuin 1 (Sirt1) and Sirt2, play crucial roles in many biological processes, including cell proliferation, differentiation and apoptosis. HDAC inhibitors have been considered as a potential therapeutic approach for various types of cancers. Here, we demonstrated that the Sirt1 and Sirt2 inhibitors EX527 and AGK2 suppressed cell growth and caused G1 phase arrest by inhibiting the expression of Cdk6 and/or Cdk4. An agar colony formation assay revealed that EX527 and AGK2 decreased colony formation in soft agar. Furthermore, EX527 and AGK2 pretreatment inhibited the expression of HSF1 and HSP27 and induced HSF1 ubiquitination. Sirt1 overexpression increased HSF1 expression and/or stabilization and induced cell migration in a scratch assay. Overall, these results indicate that EX527 and AGK2 suppress cell growth and migration by inhibiting HSF1 protein stability.
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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Kim S and Ahn S: Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncol Rep 35: 235-242, 2016.
APA
Kim, H., Kim, S., & Ahn, S. (2016). Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncology Reports, 35, 235-242. https://doi.org/10.3892/or.2015.4381
MLA
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35.1 (2016): 235-242.
Chicago
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35, no. 1 (2016): 235-242. https://doi.org/10.3892/or.2015.4381
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Kim S and Ahn S: Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncol Rep 35: 235-242, 2016.
APA
Kim, H., Kim, S., & Ahn, S. (2016). Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncology Reports, 35, 235-242. https://doi.org/10.3892/or.2015.4381
MLA
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35.1 (2016): 235-242.
Chicago
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35, no. 1 (2016): 235-242. https://doi.org/10.3892/or.2015.4381
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