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Article

Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer

  • Authors:
    • Xiao-Dong Yang
    • Hong-Tao Xu
    • Xiao-Hui Xu
    • Gan Ru
    • Wei Liu
    • Jun-Jia Zhu
    • Yong-You Wu
    • Kui Zhao
    • Yong Wu
    • Chun-Gen Xing
    • Shu-Yu Zhang
    • Jian-Ping Cao
    • Ming Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of General Surgery, The First People's Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Taicang, Jiangsu 215400, P.R. China, Department of General Surgery, Hospital of Suzhou Science and Technology Town, Suzhou, Jiangsu 215000, P.R. China, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China
  • Pages: 479-487
    |
    Published online on: November 4, 2015
       https://doi.org/10.3892/or.2015.4397
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Abstract

Colorectal cancer (CRC) is still one of the most important neoplasias causing human death. Multidisciplinary therapy has won consensus in the management of CRC, of which, radiotherapy occupies an important position. However, radioresistance is still a major obstacle in local control of CRC. Overexpression of long non-coding RNA HOTAIR has been found to correlate with tumorigenesis and poor prognosis in several types of cancer. In the present study, we analyzed HOTAIR expression levels of 53 CRC patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of HOTAIR by RNA interference was performed to explore its roles in cell proliferation, migration, invasion, apoptosis and radiosensitivity. Results showed that CRC patients had higher HOTAIR expression in tumor tissues compared with adjacent normal tissues. In vitro, downregulation of HOTAIR reduced proliferation, migration and invasiveness while enhanced apoptosis and radio­sensitivity of CRC cells. Taken together, our findings suggest that long non-coding RNA HOTAIR expression is closely associated with tumor invasion and radiosensitivity, indicating the potential role in diagnostics and therapeutics of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Xu H, Xu X, Ru G, Liu W, Zhu J, Wu Y, Zhao K, Wu Y, Xing C, Xing C, et al: Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer. Oncol Rep 35: 479-487, 2016.
APA
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J. ... Li, M. (2016). Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer. Oncology Reports, 35, 479-487. https://doi.org/10.3892/or.2015.4397
MLA
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J., Wu, Y., Zhao, K., Wu, Y., Xing, C., Zhang, S., Cao, J., Li, M."Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer". Oncology Reports 35.1 (2016): 479-487.
Chicago
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J., Wu, Y., Zhao, K., Wu, Y., Xing, C., Zhang, S., Cao, J., Li, M."Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer". Oncology Reports 35, no. 1 (2016): 479-487. https://doi.org/10.3892/or.2015.4397
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Xu H, Xu X, Ru G, Liu W, Zhu J, Wu Y, Zhao K, Wu Y, Xing C, Xing C, et al: Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer. Oncol Rep 35: 479-487, 2016.
APA
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J. ... Li, M. (2016). Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer. Oncology Reports, 35, 479-487. https://doi.org/10.3892/or.2015.4397
MLA
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J., Wu, Y., Zhao, K., Wu, Y., Xing, C., Zhang, S., Cao, J., Li, M."Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer". Oncology Reports 35.1 (2016): 479-487.
Chicago
Yang, X., Xu, H., Xu, X., Ru, G., Liu, W., Zhu, J., Wu, Y., Zhao, K., Wu, Y., Xing, C., Zhang, S., Cao, J., Li, M."Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer". Oncology Reports 35, no. 1 (2016): 479-487. https://doi.org/10.3892/or.2015.4397
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