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Article

Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells

  • Authors:
    • Guangming Zhao
    • Xiaodong Han
    • Siwen Zheng
    • Zhen Li
    • Yang Sha
    • Jing Ni
    • Zhe Sun
    • Song Qiao
    • Zhiqi Song
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 1065-1074
    |
    Published online on: November 12, 2015
       https://doi.org/10.3892/or.2015.4413
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Abstract

Melanoma is the foremost malignant cutaneous cancer and it is extremely resistant to chemotherapy and radiotherapy. Curcumin is an active component of turmeric, the yellow spice derived from the rhizome of Curcuma longa, and is widely known for its anti-inflammatory and anti-cancerogenic properties. Several recent studies suggest that curcumin induces apoptosis by modulating multiple signaling pathways to exert its anticancer effect. In the present study, we investigated the effect of curcumin on the viability, invasion potential, cell cycle, autophagy and the AKT, mTOR, P70S6K proteins of AKT/mTOR signaling pathway in human melanoma A375 and C8161 cell lines in vitro and in an in vivo tumorigenesis model. Curcumin effectively inhibited the proliferation of melanoma cells in vitro and in vivo. It suppressed cell invasion, arrested the cancer cells at G2/M phase of the cell cycle, and induced autophagy. Furthermore, curcumin suppressed the activation of AKT, mTOR and P70S6K proteins. Curcumin, therefore, is a potent suppressor of cell viability and invasion, and simultaneously an inducer of autophagy in A375 and C8161 cells. Accordingly, curcumin could be a novel therapeutic candidate for the management of melanoma.
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1 

Russo A, Ficili B, Candido S, Pezzino FM, Guarneri C, Biondi A, Travali S, McCubrey JA, Spandidos DA and Libra M: Emerging targeted therapies for melanoma treatment (Review). Int J Oncol. 45:516–524. 2014.PubMed/NCBI

2 

Voskoboynik M and Arkenau HT: Combination therapies for the treatment of advanced melanoma: A review of current evidence. Biochem Res Int. 2014:3070592014. View Article : Google Scholar : PubMed/NCBI

3 

Anand P, Sundaram C, Jhurani S, Kunnumakkara AB and Aggarwal BB: Curcumin and cancer: An 'old-age' disease with an 'age-old' solution. Cancer Lett. 267:133–164. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Chatterjee SJ and Pandey S: Chemo-resistant melanoma sensitized by tamoxifen to low dose curcumin treatment through induction of apoptosis and autophagy. Cancer Biol Ther. 11:216–228. 2011. View Article : Google Scholar

5 

Siwak DR, Shishodia S, Aggarwal BB and Kurzrock R: Curcumin-induced antiproliferative and proapoptotic effects in melanoma cells are associated with suppression of IkappaB kinase and nuclear factor kappaB activity and are independent of the B-Raf/mitogen-activated/extracellular signal-regulated protein kinase pathway and the Akt pathway. Cancer. 104:879–890. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Zheng M, Ekmekcioglu S, Walch ET, Tang CH and Grimm EA: Inhibition of nuclear factor-kappaB and nitric oxide by curcumin induces G2/M cell cycle arrest and apoptosis in human melanoma cells. Melanoma Res. 14:165–171. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Odot J, Albert P, Carlier A, Tarpin M, Devy J and Madoulet C: In vitro and in vivo anti-tumoral effect of curcumin against melanoma cells. Int J Cancer. 111:381–387. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Marín YE, Wall BA, Wang S, Namkoong J, Martino JJ, Suh J, Lee HJ, Rabson AB, Yang CS, Chen S, et al: Curcumin downregulates the constitutive activity of NF-kappaB and induces apoptosis in novel mouse melanoma cells. Melanoma Res. 17:274–283. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Oelkrug C, Lange CM, Wenzel E, Fricke S, Hartke M, Simasi J and Schubert A: Analysis of the tumoricidal and anti-cachectic potential of curcumin. Anticancer Res. 34:4781–4788. 2014.PubMed/NCBI

10 

Anand P, Kunnumakkara AB, Newman RA and Aggarwal BB: Bioavailability of curcumin: Problems and promises. Mol Pharm. 4:807–818. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Liu H, He Z and Simon HU: Targeting autophagy as a potential therapeutic approach for melanoma therapy. Semin Cancer Biol. 23:352–360. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Hönscheid P, Datta K and Muders MH: Autophagy: Detection, regulation and its role in cancer and therapy response. Int J Radiat Biol. 90:628–635. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Chu YL, Raghu R, Lu KH, Liu CT, Lin SH, Lai YS, Cheng WC, Lin SH and Sheen LY: Autophagy therapeutic potential of garlic in human cancer therapy. J Tradit Complement Med. 3:159–162. 2013. View Article : Google Scholar

14 

Thorburn A, Thamm DH and Gustafson DL: Autophagy and cancer therapy. Mol Pharmacol. 85:830–838. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Alifrangis C, Koizia L, Rozario A, Rodney S, Harrington M, Somerville C, Peplow T and Waxman J: The experiences of cancer patients. QJM. 104:1075–1081. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Slevin ML, Stubbs L, Plant HJ, Wilson P, Gregory WM, Armes PJ and Downer SM: Attitudes to chemotherapy: Comparing views of patients with cancer with those of doctors, nurses, and general public. BMJ. 300:1458–1460. 1990. View Article : Google Scholar : PubMed/NCBI

18 

Thornton M, Parry M, Gill P, Mead D and Macbeth F: Hard choices: A qualitative study of influences on the treatment decisions made by advanced lung cancer patients. Int J Palliat Nurs. 17:68–74. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Chinembiri TN, du Plessis LH, Gerber M, Hamman JH and du Plessis J: Review of natural compounds for potential skin cancer treatment. Molecules. 19:11679–11721. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Stewart ZA, Westfall MD and Pietenpol JA: Cell-cycle dysregulation and anticancer therapy. Trends Pharmacol Sci. 24:139–145. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Cho JH, Lee JG, Yang YI, Kim JH, Ahn JH, Baek NI, Lee KT and Choi JH: Eupatilin, a dietary flavonoid, induces G2/M cell cycle arrest in human endometrial cancer cells. Food Chem Toxicol. 49:1737–1744. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Rozzo C, Fanciulli M, Fraumene C, Corrias A, Cubeddu T, Sassu I, Cossu S, Nieddu V, Galleri G, Azara E, et al: Molecular changes induced by the curcumin analogue D6 in human melanoma cells. Mol Cancer. 12:372013. View Article : Google Scholar : PubMed/NCBI

23 

Zhang L, Wang H, Xu J, Zhu J and Ding K: Inhibition of cathepsin S induces autophagy and apoptosis in human glioblastoma cell lines through ROS-mediated PI3K/AKT/mTOR/p70S6K and JNK signaling pathways. Toxicol Lett. 228:248–259. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Ornelas IM, Silva TM, Fragel-Madeira L and Ventura AL: Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina. PLoS One. 8:e535172013. View Article : Google Scholar : PubMed/NCBI

25 

Hennessy BT, Smith DL, Ram PT, Lu Y and Mills GB: Exploiting the PI3K/AKT pathway for cancer drug discovery. Nat Rev Drug Discov. 4:988–1004. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Lin HP, Jiang SS and Chuu CP: Caffeic acid phenethyl ester causes p21 induction, Akt signaling reduction, and growth inhibition in PC-3 human prostate cancer cells. PLoS One. 7:e312862012. View Article : Google Scholar : PubMed/NCBI

27 

Zhou HY and Wong AS: Activation of p70S6K induces expression of matrix metalloproteinase 9 associated with hepatocyte growth factor-mediated invasion in human ovarian cancer cells. Endocrinology. 147:2557–2566. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Berven LA, Willard FS and Crouch MF: Role of the p70S6K pathway in regulating the actin cytoskeleton and cell migration. Exp Cell Res. 296:183–195. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Corazzari M, Fimia GM, Lovat P and Piacentini M: Why is autophagy important for melanoma? Molecular mechanisms and therapeutic implications. Semin Cancer Biol. 23:337–343. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Wu S, Wang X, Chen J and Chen Y: Autophagy of cancer stem cells is involved with chemoresistance of colon cancer cells. Biochem Biophys Res Commun. 434:898–903. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Han Z, Jing Y, Xia Y, Zhang S, Hou J, Meng Y, Yu F, Liu X, Wu M, Zhang P, et al: Mesenchymal stem cells contribute to the chemoresistance of hepatocellular carcinoma cells in inflammatory environment by inducing autophagy. Cell Biosci. 4:222014. View Article : Google Scholar : PubMed/NCBI

32 

Ge J and Chen Z, Huang J, Chen J, Yuan W, Deng Z and Chen Z: Upregulation of autophagy-related gene-5 (ATG-5) is associated with chemoresistance in human gastric cancer. PLoS One. 9:e1102932014. View Article : Google Scholar : PubMed/NCBI

33 

Armstrong JL, Corazzari M, Martin S, Pagliarini V, Falasca L, Hill DS, Ellis N, Al Sabah S, Redfern CP, Fimia GM, et al: Oncogenic B-RAF signaling in melanoma impairs the therapeutic advantage of autophagy inhibition. Clin Cancer Res. 17:2216–2226. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Janku F, McConkey DJ, Hong DS and Kurzrock R: Autophagy as a target for anticancer therapy. Nat Rev Clin Oncol. 8:528–539. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao G, Han X, Zheng S, Li Z, Sha Y, Ni J, Sun Z, Qiao S and Song Z: Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells. Oncol Rep 35: 1065-1074, 2016.
APA
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J. ... Song, Z. (2016). Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells. Oncology Reports, 35, 1065-1074. https://doi.org/10.3892/or.2015.4413
MLA
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J., Sun, Z., Qiao, S., Song, Z."Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells". Oncology Reports 35.2 (2016): 1065-1074.
Chicago
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J., Sun, Z., Qiao, S., Song, Z."Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells". Oncology Reports 35, no. 2 (2016): 1065-1074. https://doi.org/10.3892/or.2015.4413
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao G, Han X, Zheng S, Li Z, Sha Y, Ni J, Sun Z, Qiao S and Song Z: Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells. Oncol Rep 35: 1065-1074, 2016.
APA
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J. ... Song, Z. (2016). Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells. Oncology Reports, 35, 1065-1074. https://doi.org/10.3892/or.2015.4413
MLA
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J., Sun, Z., Qiao, S., Song, Z."Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells". Oncology Reports 35.2 (2016): 1065-1074.
Chicago
Zhao, G., Han, X., Zheng, S., Li, Z., Sha, Y., Ni, J., Sun, Z., Qiao, S., Song, Z."Curcumin induces autophagy, inhibits proliferation and invasion by downregulating AKT/mTOR signaling pathway in human melanoma cells". Oncology Reports 35, no. 2 (2016): 1065-1074. https://doi.org/10.3892/or.2015.4413
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