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Article

Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells

Retraction in: /10.3892/or.2025.9032
  • Authors:
    • Xinhua Xu
    • Yang Liu
    • Jin Su
    • Daojun Li
    • Juan Hu
    • Qiao Huang
    • Mingqian Lu
    • Xiaoyan Liu
    • Jinghua Ren
    • Weihong Chen
    • Lidan Sun
  • View Affiliations / Copyright

    Affiliations: The First College of Clinical Medical Science, China Three Gorges University and Department of Oncology, Yichang Central People's Hospital, Yichang, Hubei 443003, P.R. China, Oncology Institute, China Three Gorges University, Yichang, Hubei 443003, P.R. China, Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430023, P.R. China, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Department of Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China
  • Pages: 923-931
    |
    Published online on: November 12, 2015
       https://doi.org/10.3892/or.2015.4414
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Abstract

Bmi-1 (B-cell-specific Moloney murine leukemia virus insertion site 1) is a member of the Polycomb group gene (PcG) family, which is involved in the proliferation, migration and tumorigenesis of several types of cancer stem cells (CSCs). However, its precise role and mechanism in CD44+ nasopharyngeal carcinoma (NPC) cancer stem-like cells (CSC-LCs) remain poorly understood. In our previous study, we successfully silenced Bmi-1 by short hairpin RNA (shRNA) in CD44+ NPC CSC-LCs and obtained stable Bmi-1 knockdown (KD) cell lines. In the present study, we tested the cell proliferation by CCK-8 assay and apoptosis by flow cytometry. Scratch wound healing assay, together with Transwell migration and invasion assays were used to measure the migration and invasion capacity. We further evaluated the tumorigenicity of CD44+ NPC CSC-LCs transfected with Bmi-1 shRNA in vivo. Based on our results, knockdown of Bmi-1 by shRNA resulted in the inhibition of tumor proliferation, migration and invasion in vitro, followed by cell apoptosis. In addition, our results preliminarily demonstrated that inhibition of Bmi-1 expression by shRNA increased tumor apoptosis through the p16INK4a-p14ARF-p53 pathway. Bmi-1 silencing in CD44+ NPC CSC-LCs also resulted in the failure to develop tumors in vivo. These results provide important insights into the role of Bmi-1 in the occurrence and development of NPC. Based on our findings, regulation of Bmi-1 in CD44+ NPC CSC-LCs may provide a potential molecular target for the therapy of NPC, and targeted silencing of Bmi-1 by shRNA may have clinical future implications in NPC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Xu X, Liu Y, Su J, Li D, Hu J, Huang Q, Lu M, Liu X, Ren J, Chen W, Chen W, et al: Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032. Oncol Rep 35: 923-931, 2016.
APA
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q. ... Sun, L. (2016). Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032. Oncology Reports, 35, 923-931. https://doi.org/10.3892/or.2015.4414
MLA
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q., Lu, M., Liu, X., Ren, J., Chen, W., Sun, L."Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032". Oncology Reports 35.2 (2016): 923-931.
Chicago
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q., Lu, M., Liu, X., Ren, J., Chen, W., Sun, L."Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032". Oncology Reports 35, no. 2 (2016): 923-931. https://doi.org/10.3892/or.2015.4414
Copy and paste a formatted citation
x
Spandidos Publications style
Xu X, Liu Y, Su J, Li D, Hu J, Huang Q, Lu M, Liu X, Ren J, Chen W, Chen W, et al: Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032. Oncol Rep 35: 923-931, 2016.
APA
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q. ... Sun, L. (2016). Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032. Oncology Reports, 35, 923-931. https://doi.org/10.3892/or.2015.4414
MLA
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q., Lu, M., Liu, X., Ren, J., Chen, W., Sun, L."Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032". Oncology Reports 35.2 (2016): 923-931.
Chicago
Xu, X., Liu, Y., Su, J., Li, D., Hu, J., Huang, Q., Lu, M., Liu, X., Ren, J., Chen, W., Sun, L."Downregulation of Bmi-1 is associated with suppressed tumorigenesis and induced apoptosis in CD44+ nasopharyngeal carcinoma cancer stem-like cells Retraction in /10.3892/or.2025.9032". Oncology Reports 35, no. 2 (2016): 923-931. https://doi.org/10.3892/or.2015.4414
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