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Article

Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9

  • Authors:
    • Min Xiong
    • Lin Wang
    • Hua‑Long Yu
    • Heng Han
    • Dan Mao
    • Jie Chen
    • Yun Zeng
    • Ning He
    • Zhi‑Gang Liu
    • Zhi‑Yong Wang
    • Shou‑Jia Xu
    • Le-Yun Guo
    • Yong-An Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, P.R. China
  • Pages: 1034-1040
    |
    Published online on: November 16, 2015
       https://doi.org/10.3892/or.2015.4427
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Abstract

Osteosarcoma is composed of tumor osteoblasts and bone-like tissues, with malignant tumors originating from osteogenesis organization. Osteosarcoma is a primary malignant bone tumor. Invasion and metastasis of osteosarcoma affect the prognosis of patients. However, effective therapeutic treatments remain to be identified. The aim of the present study was to investigate the possible inhibitory and apoptotic effects of ginkgetin in osteosarcoma cells. 3.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were used to determine the effect ginkgetin exerted on the growth of osteosarcoma cells. Flow cytometry was used to determine cell apoptosis. STAT3 protein expression and activation of caspase-3/9 were measured using western blot analysis and the MTT and LDH assays, respectively. The results showed that ginkgetin inhibited cell growth and induced cell cytotoxicity in osteosarcoma cells in a dose-dependent manner. Treatment with ginkgetin significantly activated the apoptosis of osteosarcoma cells in a concentration-dependent manner. The anticancer activity of ginkgetin significantly inhibited STAT3 and promoted caspase-3/9 activation in osteosarcoma cells. The findings demonstrated that ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through the inhibition of STAT3 and activation of caspase-3/9.
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View References

1 

Bacci G, Gherlinzoni F, Picci P, et al: Adriamycin-methotrexate high dose versus adriamycin-methotrexate moderate dose as adjuvant chemotherapy for osteosarcoma of the extremities: a randomized study. Eur J Cancer Clin Oncol. 22:1337–1345. 1986. View Article : Google Scholar : PubMed/NCBI

2 

Burdach S, van Kaick B, Laws HJ, Ahrens S, Haase R, Körholz D, Pape H, Dunst J, Kahn T, Willers R, et al: Allogeneic and autologous stem-cell transplantation in advanced Ewing tumors. An update after long-term follow-up from two centers of the European Intergroup study EICESS Stem-Cell Transplant Programs at Düsseldorf University Medical Center, Germany and St Anna Kinderspital, Vienna, Austria. Ann Oncol. 11:1451–1462. 2000. View Article : Google Scholar

3 

Kong CB, Kim MS, Lee SY, Cho WH, Song WS, Lee JA, Yoo JY, Chung SH and Jeon DG: Prognostic effect of diaphyseal location in osteosarcoma: A cohort case-control study at a single institute. Ann Surg Oncol. 16:3094–3100. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Ferrari S, Smeland S, Mercuri M, Bertoni F, Longhi A, Ruggieri P, Alvegard TA, Picci P, Capanna R, Bernini G, et al Italian and Scandinavian Sarcoma Groups: Neoadjuvant chemotherapy with high-dose ifosfamide, high-dose methotrexate, cisplatin, and doxorubicin for patients with localize1d osteosarcoma of the extremity: A joint study by the Italian and Scandinavian Sarcoma Groups. J Clin Oncol. 23:8845–8852. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Lee JA, Choi SY, Kang HJ, Lee JW, Kim H, Kim JH, Sung KW, Shin HY, Ahn HS and Park KD: Treatment outcome of osteosarcoma after bilateral retinoblastoma: A retrospective study of eight cases. Br J Ophthalmol. 98:1355–1359. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Su Y, Sun CM, Chuang HH and Chang PT: Studies on the cytotoxic mechanisms of ginkgetin in a human ovarian adenocarcinoma cell line. Naunyn Schmiedebergs Arch Pharmacol. 362:82–90. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Wang YQ, Wang MY, Fu XR, Peng-Yu, Gao GF, Fan YM, Duan XL, Zhao BL, Chang YZ and Shi ZH: Neuroprotective effects of ginkgetin against neuro-injury in Parkinson's disease model induced by MPTP via chelating iron. Free Radic Res. Jul 1–2015.Epub ahead of print.

8 

Lim H, Son KH, Chang HW, Kang SS and Kim HP: Effects of anti-inflammatory biflavonoid, ginkgetin, on chronic skin inflammation. Biol Pharm Bull. 29:1046–1049. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Zhang Y, Shi S, Wang Y and Huang K: Target-guided isolation and purification of antioxidants from Selaginella sinensis by offline coupling of DPPH-HPLC and HSCCC experiments. J Chromatogr B Analyt Technol Biomed Life Sci. 879:191–196. 2011. View Article : Google Scholar

10 

You OH and Kim SH, Kim B, Sohn EJ, Lee HJ, Shim BS, Yun M, Kwon BM and Kim SH: Ginkgetin induces apoptosis via activation of caspase and inhibition of survival genes in PC-3 prostate cancer cells. Bioorg Med Chem Lett. 23:2692–2695. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Iwata S, Ishii T, Kawai A, Hiruma T, Yonemoto T, Kamoda H, Asano N and Takeyama M: Prognostic factors in elderly osteosarcoma patients: A multi-institutional retrospective study of 86 cases. Ann Surg Oncol. 21:263–268. 2014. View Article : Google Scholar

12 

Boye K, Del Prever AB, Eriksson M, Saeter G, Tienghi A, Lindholm P, Fagioli F, Skjeldal S, Ferrari S and Hall KS: High-dose chemotherapy with stem cell rescue in the primary treatment of metastatic and pelvic osteosarcoma: Final results of the ISG/SSG II study. Pediatr Blood Cancer. 61:840–845. 2014. View Article : Google Scholar

13 

Wang X, Goldstein D, Crowe PJ and Yang JL: Impact of STAT3 inhibition on survival of osteosarcoma cell lines. Anticancer Res. 34:6537–6545. 2014.PubMed/NCBI

14 

Salas S, Jiguet-Jiglaire C, Campion L, Bartoli C, Frassineti F, Deville JL, Maues De Paula A, Forest F, Jézéquel P, Gentet JC, et al: Correlation between ERK1 and STAT3 expression and chemoresistance in patients with conventional osteosarcoma. BMC Cancer. 14:6062014. View Article : Google Scholar : PubMed/NCBI

15 

Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, et al: Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. Nat Commun. 6:70952015. View Article : Google Scholar : PubMed/NCBI

16 

Yu H, Lee H, Herrmann A, Buettner R and Jove R: Revisiting STAT3 signalling in cancer: New and unexpected biological functions. Nat Rev Cancer. 14:736–746. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Liu J, Wei W, Guo CA, Han N, Pan JF, Fei T and Yan ZQ: Stat3 upregulates leucine-rich repeat-containing g protein-coupled receptor 4 expression in osteosarcoma cells. Biomed Res Int. 2013:3106912013.

18 

Jeon YJ, Jung SN, Yun J, Lee CW, Choi J, Lee YJ, Han DC and Kwon BM: Ginkgetin inhibits the growth of DU-145 prostate cancer cells through inhibition of signal transducer and activator of transcription 3 activity. Cancer Sci. 106:413–420. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Huang J, Lyu H, Wang J and Liu B: MicroRNA regulation and therapeutic targeting of survivin in cancer. Am J Cancer Res. 5:20–31. 2015.PubMed/NCBI

20 

Nemec KN and Khaled AR: Therapeutic modulation of apoptosis: Targeting the BCL-2 family at the interface of the mitochondrial membrane. Yonsei Med J. 49:689–697. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Rautureau GJ, Day CL and Hinds MG: Intrinsically disordered proteins in bcl-2 regulated apoptosis. Int J Mol Sci. 11:1808–1824. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Jiang L, Wang P, Chen L and Chen H: Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. Int J Clin Exp Pathol. 7:5450–5460. 2014.PubMed/NCBI

23 

Ma YP, Zou P, Xiao J and Huang SA: Expression of survivin in cord blood CD34+ stem/progenitor cells and its significance. Zhonghua Xue Ye Xue Za Zhi. 24:238–240. 2003.In Chinese. PubMed/NCBI

24 

Langelier MF and Pascal JM: PARP-1 mechanism for coupling DNA damage detection to poly(ADP-ribose) synthesis. Curr Opin Struct Biol. 23:134–143. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Griffin RJ, Pemberton LC, Rhodes D, Bleasdale C, Bowman K, Calvert AH, Curtin NJ, Durkacz BW, Newell DR, Porteous JK, et al: Novel potent inhibitors of the DNA repair enzyme poly(ADP-ribose)polymerase (PARP). Anticancer Drug Des. 10:507–514. 1995.PubMed/NCBI

26 

Yang F, Nam S, Zhao R, Tian Y, Liu L, Horne DA and Jove R: A novel synthetic derivative of the natural product berbamine inhibits cell viability and induces apoptosis of human osteosarcoma cells, associated with activation of JNK/AP-1 signaling. Cancer Biol Ther. 14:1024–1031. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xiong M, Wang L, Yu HL, Han H, Mao D, Chen J, Zeng Y, He N, Liu ZG, Wang ZY, Wang ZY, et al: Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep 35: 1034-1040, 2016.
APA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J. ... Wang, Y. (2016). Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncology Reports, 35, 1034-1040. https://doi.org/10.3892/or.2015.4427
MLA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35.2 (2016): 1034-1040.
Chicago
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35, no. 2 (2016): 1034-1040. https://doi.org/10.3892/or.2015.4427
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong M, Wang L, Yu HL, Han H, Mao D, Chen J, Zeng Y, He N, Liu ZG, Wang ZY, Wang ZY, et al: Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep 35: 1034-1040, 2016.
APA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J. ... Wang, Y. (2016). Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncology Reports, 35, 1034-1040. https://doi.org/10.3892/or.2015.4427
MLA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35.2 (2016): 1034-1040.
Chicago
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35, no. 2 (2016): 1034-1040. https://doi.org/10.3892/or.2015.4427
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