Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9

  • Authors:
    • Min Xiong
    • Lin Wang
    • Hua‑Long Yu
    • Heng Han
    • Dan Mao
    • Jie Chen
    • Yun Zeng
    • Ning He
    • Zhi‑Gang Liu
    • Zhi‑Yong Wang
    • Shou‑Jia Xu
    • Le-Yun Guo
    • Yong-An Wang
  • View Affiliations

  • Published online on: November 16, 2015     https://doi.org/10.3892/or.2015.4427
  • Pages: 1034-1040
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Abstract

Osteosarcoma is composed of tumor osteoblasts and bone-like tissues, with malignant tumors originating from osteogenesis organization. Osteosarcoma is a primary malignant bone tumor. Invasion and metastasis of osteosarcoma affect the prognosis of patients. However, effective therapeutic treatments remain to be identified. The aim of the present study was to investigate the possible inhibitory and apoptotic effects of ginkgetin in osteosarcoma cells. 3.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were used to determine the effect ginkgetin exerted on the growth of osteosarcoma cells. Flow cytometry was used to determine cell apoptosis. STAT3 protein expression and activation of caspase-3/9 were measured using western blot analysis and the MTT and LDH assays, respectively. The results showed that ginkgetin inhibited cell growth and induced cell cytotoxicity in osteosarcoma cells in a dose-dependent manner. Treatment with ginkgetin significantly activated the apoptosis of osteosarcoma cells in a concentration-dependent manner. The anticancer activity of ginkgetin significantly inhibited STAT3 and promoted caspase-3/9 activation in osteosarcoma cells. The findings demonstrated that ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through the inhibition of STAT3 and activation of caspase-3/9.
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February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xiong M, Wang L, Yu HL, Han H, Mao D, Chen J, Zeng Y, He N, Liu ZG, Wang ZY, Wang ZY, et al: Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep 35: 1034-1040, 2016
APA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J. ... Wang, Y. (2016). Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncology Reports, 35, 1034-1040. https://doi.org/10.3892/or.2015.4427
MLA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35.2 (2016): 1034-1040.
Chicago
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35, no. 2 (2016): 1034-1040. https://doi.org/10.3892/or.2015.4427