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Article

Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins

  • Authors:
    • Bashir A. Yousef
    • Mounia Guerram
    • Hozeifa M. Hassan
    • Aida M. Hamdi
    • Lu-Yong Zhang
    • Zhen-Zhou Jiang
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 1091-1100
    |
    Published online on: November 26, 2015
       https://doi.org/10.3892/or.2015.4457
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Abstract

Pristimerin is a naturally occurring triterpenoid that has a cytotoxic effect on several cancer cell lines. However, the cytotoxic effects of pristimerin as well as its molecular mechanisms of action against colorectal cancer have never been explored. In the present study, we investigated the anticancer potential of pristimerin, and examined the different signaling pathways affected by its action in three colon cancer cell lines namely HCT-116, COLO-205 and SW-620. Pristimerin was found to possess potent cytotoxic and proliferation inhibitory effects against these cell lines. Cell cycle analysis revealed G1 phase arrest, which was strongly associated with decreased expression of cyclin D1 and cyclin-dependent kinases (cdk4 and cdk6) with concomitant induction of p21. Pristimerin also induced apoptosis in a dose-dependent manner. Cell plasma membrane alterations studied by Annexin V/PI double staining, loss of mitochondrial membrane potential (ΔΨm), measurements of caspase activities and the inhibitory effect of Z-VAD-FMK (a caspase inhibitor) confirmed the apoptotic effect of pristimerin. Moreover, western blot data showed that apoptotic induction was associated with activated caspase-3 and -8, PARP-1 cleavage and modulation of the expression levels of Bcl-2 family proteins. Additionally, pristimerin treatment downregulated the phosphorylated forms of EGFR and HER2 proteins, and subsequently caused a decrease in the phosphorylated forms of Erk1/2, Akt, mTOR and NF-κB proteins. Taken together, these results suggest that pristimerin may have potential as a new targeting therapeutic strategy for the treatment of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yousef BA, Guerram M, Hassan HM, Hamdi AM, Zhang L and Jiang Z: Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins. Oncol Rep 35: 1091-1100, 2016.
APA
Yousef, B.A., Guerram, M., Hassan, H.M., Hamdi, A.M., Zhang, L., & Jiang, Z. (2016). Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins. Oncology Reports, 35, 1091-1100. https://doi.org/10.3892/or.2015.4457
MLA
Yousef, B. A., Guerram, M., Hassan, H. M., Hamdi, A. M., Zhang, L., Jiang, Z."Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins". Oncology Reports 35.2 (2016): 1091-1100.
Chicago
Yousef, B. A., Guerram, M., Hassan, H. M., Hamdi, A. M., Zhang, L., Jiang, Z."Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins". Oncology Reports 35, no. 2 (2016): 1091-1100. https://doi.org/10.3892/or.2015.4457
Copy and paste a formatted citation
x
Spandidos Publications style
Yousef BA, Guerram M, Hassan HM, Hamdi AM, Zhang L and Jiang Z: Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins. Oncol Rep 35: 1091-1100, 2016.
APA
Yousef, B.A., Guerram, M., Hassan, H.M., Hamdi, A.M., Zhang, L., & Jiang, Z. (2016). Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins. Oncology Reports, 35, 1091-1100. https://doi.org/10.3892/or.2015.4457
MLA
Yousef, B. A., Guerram, M., Hassan, H. M., Hamdi, A. M., Zhang, L., Jiang, Z."Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins". Oncology Reports 35.2 (2016): 1091-1100.
Chicago
Yousef, B. A., Guerram, M., Hassan, H. M., Hamdi, A. M., Zhang, L., Jiang, Z."Pristimerin demonstrates anticancer potential in colorectal cancer cells by inducing G1 phase arrest and apoptosis and suppressing various pro-survival signaling proteins". Oncology Reports 35, no. 2 (2016): 1091-1100. https://doi.org/10.3892/or.2015.4457
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