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Article

Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma

Expression of Concern in: /10.3892/or.2025.8997
  • Authors:
    • Da Huang
    • Xiaobei Wang
    • Chunbo Zhuang
    • Wuhe Shi
    • Mu Liu
    • Qiming Tu
    • Detai Zhang
    • Lihua Hu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 1083-1090
    |
    Published online on: December 1, 2015
       https://doi.org/10.3892/or.2015.4467
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Abstract

Although the tumor suppressive role of miR-101 is well documented in hepatocellular carcinoma (HCC), how the expression of miR-101 itself is regulated remains elusive. In the present study, we demonstrated that the miR-101 precursor pre-miR-101-1 could be regulated by an important epigenetic regulator, the enhancer of zeste homolog 2 (EZH2). Reporter gene assays revealed that ectopic expression of EZH2 inhibited the transcriptional activities of miR-101-1 promoter. Subsequent analyses revealed that miR-101-1 directly represses the expression of EZH2, and miR-101-1 and EZH2 form a reciprocal negative feedback loop as indicated by the fact that ectopic mature miR-101 could induce endogenous pre-miR-101-1 expression. This mature miR-101-induced pre-miR-101 expression was specific to pre-miR-101-1 and depended on EZH2 activities. Moreover, our results also demonstrated that similar antitumor effects can be achieved either by ectopic miR-101 or EZH2 silencing in HCC cells. These findings show that elevated EZH2 contributes to miR-101 deregulation in HCC and highlight the coordinated role of miR-101 and EZH2 in hepatocarcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Huang D, Wang X, Zhuang C, Shi W, Liu M, Tu Q, Zhang D and Hu L: Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997. Oncol Rep 35: 1083-1090, 2016.
APA
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q. ... Hu, L. (2016). Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997. Oncology Reports, 35, 1083-1090. https://doi.org/10.3892/or.2015.4467
MLA
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q., Zhang, D., Hu, L."Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997". Oncology Reports 35.2 (2016): 1083-1090.
Chicago
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q., Zhang, D., Hu, L."Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997". Oncology Reports 35, no. 2 (2016): 1083-1090. https://doi.org/10.3892/or.2015.4467
Copy and paste a formatted citation
x
Spandidos Publications style
Huang D, Wang X, Zhuang C, Shi W, Liu M, Tu Q, Zhang D and Hu L: Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997. Oncol Rep 35: 1083-1090, 2016.
APA
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q. ... Hu, L. (2016). Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997. Oncology Reports, 35, 1083-1090. https://doi.org/10.3892/or.2015.4467
MLA
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q., Zhang, D., Hu, L."Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997". Oncology Reports 35.2 (2016): 1083-1090.
Chicago
Huang, D., Wang, X., Zhuang, C., Shi, W., Liu, M., Tu, Q., Zhang, D., Hu, L."Reciprocal negative feedback loop between EZH2 and miR-101-1 contributes to miR-101 deregulation in hepatocellular carcinoma Expression of Concern in /10.3892/or.2025.8997". Oncology Reports 35, no. 2 (2016): 1083-1090. https://doi.org/10.3892/or.2015.4467
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