Rituximab-conjugated and doxorubicin-loaded microbubbles combined with ultrasound irradiation inhibits proliferation and induces apoptosis in Raji cell lines

  • Authors:
    • Shoubing Zhou
    • Shiya Zheng
    • Yongfeng Shan
    • Lulu Li
    • Xiu Zhang
    • Cailian Wang
  • View Affiliations

  • Published online on: December 1, 2015     https://doi.org/10.3892/or.2015.4468
  • Pages: 801-808
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Abstract

Doxorubicin (DOX) is one of the most important medicines used for the treatment for B cell lymphoma, yet its clinical efficacy is often limited by severe adverse effects. Drug-loaded microbubbles, combined with ultrasound (US) irradiation, has shown great promise in reducing DOX-induced side effects and improving therapeutic efficacy. Nevertheless, these drug-loaded microbubbles are non-targeted microbubbles with comparatively suboptimal efficiency. Therefore, we synthesized targeted and DOX-loaded microbubbles (DMs), combined with US irradiation, for triggering drug release in lymphoma B cells. DMs were coated with rituximab via a biotin-avidin linkage to target Raji cells that overexpress the CD-20 antigen. In the present study, the cell viability after treatment with rituximab-conjugated DMs (RDMs) containing 0.25, 0.5 and 1.0 µg/ml DOX + US was 45.69±6.85, 25.31±2.60 and 15.67±2.83%, respectively, which demonstrated that RDMs + US produced significantly higher cytotoxicity than the other treatments. The early apoptosis ratio in the Raji cells at 48 h after the treatment was 32.4±2.84%, which was notably higher than the ratio in the other treatment groups. The results confirm the hypothesis that US-mediated targeting of CD-20-positive B cell lymphoma and the use of DMs may improve the DOX therapeutic efficiency.

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February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou S, Zheng S, Shan Y, Li L, Zhang X and Wang C: Rituximab-conjugated and doxorubicin-loaded microbubbles combined with ultrasound irradiation inhibits proliferation and induces apoptosis in Raji cell lines. Oncol Rep 35: 801-808, 2016
APA
Zhou, S., Zheng, S., Shan, Y., Li, L., Zhang, X., & Wang, C. (2016). Rituximab-conjugated and doxorubicin-loaded microbubbles combined with ultrasound irradiation inhibits proliferation and induces apoptosis in Raji cell lines. Oncology Reports, 35, 801-808. https://doi.org/10.3892/or.2015.4468
MLA
Zhou, S., Zheng, S., Shan, Y., Li, L., Zhang, X., Wang, C."Rituximab-conjugated and doxorubicin-loaded microbubbles combined with ultrasound irradiation inhibits proliferation and induces apoptosis in Raji cell lines". Oncology Reports 35.2 (2016): 801-808.
Chicago
Zhou, S., Zheng, S., Shan, Y., Li, L., Zhang, X., Wang, C."Rituximab-conjugated and doxorubicin-loaded microbubbles combined with ultrasound irradiation inhibits proliferation and induces apoptosis in Raji cell lines". Oncology Reports 35, no. 2 (2016): 801-808. https://doi.org/10.3892/or.2015.4468