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Article

TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells

  • Authors:
    • Ai Qing Yu
    • Yan Ding
    • Cheng Lin Li
    • Yi Yang
    • Shi Rong Yan
    • Dong Sheng Li
  • View Affiliations / Copyright

    Affiliations: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 1657-1663
    |
    Published online on: December 10, 2015
       https://doi.org/10.3892/or.2015.4483
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Abstract

Accumulating evidence indicates that Nanog plays a central role in modulating the biological behaviors of human hepatocellular carcinoma (HCC). However, the underlying mechanisms remain unclear. In the present study, we employed transcription activator-like effector nucleases (TALEN) to disrupt Nanog expression in HepG2 cells and obtained subcloned cells with diallelic Nanog mutations. Significantly, we found that the expression of pluripotency factors Sox2, Oct4 and Klf4, as well as expression of cancer stem cell (CSC) marker CD133, in the Nanog-targeted HepG2 cells was markedly downregulated. This finding suggests that Nanog may play an important role in maintaining the pluripotency and malignancy of HepG2 cells. We also revealed that Nanog regulated cell proliferation by modulating the expression of cyclin D1/D3/E1 and CDK2, respectively. Additionally, the disruption of Nanog resulted in the downregulation of epithelial-mesenchymal transition (EMT) regulators Snail and Twist, which contributed to the elevated level of epithelial marker E-cadherin, and to the decreased level of mesenchymal markers N-cadherin and vimentin in the HepG2 cells. In addition, the Nanog-targeted HepG2 cells exhibited reduced ability of invasion, migration and chemoresistance in vitro. In conclusion, the disruption of Nanog expression results in less proliferation, invasiveness, migration, more chemosensitivity and reversal of EMT in HepG2 cells, by which Nanog plays crucial roles in influencing the malignant phenotype of HepG2 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yu AQ, Ding Y, Li CL, Yang Y, Yan SR and Li DS: TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells. Oncol Rep 35: 1657-1663, 2016.
APA
Yu, A.Q., Ding, Y., Li, C.L., Yang, Y., Yan, S.R., & Li, D.S. (2016). TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells. Oncology Reports, 35, 1657-1663. https://doi.org/10.3892/or.2015.4483
MLA
Yu, A. Q., Ding, Y., Li, C. L., Yang, Y., Yan, S. R., Li, D. S."TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells". Oncology Reports 35.3 (2016): 1657-1663.
Chicago
Yu, A. Q., Ding, Y., Li, C. L., Yang, Y., Yan, S. R., Li, D. S."TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells". Oncology Reports 35, no. 3 (2016): 1657-1663. https://doi.org/10.3892/or.2015.4483
Copy and paste a formatted citation
x
Spandidos Publications style
Yu AQ, Ding Y, Li CL, Yang Y, Yan SR and Li DS: TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells. Oncol Rep 35: 1657-1663, 2016.
APA
Yu, A.Q., Ding, Y., Li, C.L., Yang, Y., Yan, S.R., & Li, D.S. (2016). TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells. Oncology Reports, 35, 1657-1663. https://doi.org/10.3892/or.2015.4483
MLA
Yu, A. Q., Ding, Y., Li, C. L., Yang, Y., Yan, S. R., Li, D. S."TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells". Oncology Reports 35.3 (2016): 1657-1663.
Chicago
Yu, A. Q., Ding, Y., Li, C. L., Yang, Y., Yan, S. R., Li, D. S."TALEN-induced disruption of Nanog expression results in reduced proliferation, invasiveness and migration, increased chemosensitivity and reversal of EMT in HepG2 cells". Oncology Reports 35, no. 3 (2016): 1657-1663. https://doi.org/10.3892/or.2015.4483
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