FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter Retraction in /10.3892/or.2023.8545

Qian,Kai; Zhang,Jinqian; Lu,Jingbo; Liu,Wenjun; Yao,Xingxing; Chen,Qing; Lu,Shun; Xiang,Guoan; Liu,Hao

doi:10.3892/or.2015.4485

March-2016 Volume 35 Issue 3

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March-2016 Volume 35 Issue 3

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Article Open Access

FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter

Retraction in: /10.3892/or.2023.8545

Authors:
- Kai Qian
- Jinqian Zhang
- Jingbo Lu
- Wenjun Liu
- Xingxing Yao
- Qing Chen
- Shun Lu
- Guoan Xiang
- Hao Liu
View Affiliations / Copyright

Affiliations: Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Laboratory Medicine, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China, Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China

Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1273-1280
|
Published online on: December 10, 2015

https://doi.org/10.3892/or.2015.4485
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Abstract

In our previous study, low expression of FAM172A protein was found in colon cancer tissues. This research was planned to explore the functions of FAM172A gene and examine the mechanisms of its transcriptional regulation. Firstly, flow cytometry showed that FAM172A inhibited proliferation and promoted apoptosis and differentiation of colon cancer cells. Then through continuous truncation, we identified the minimal functional promoter region of FAM172A. Subsequently, we found that STAT1, as a transcription factor, could bind to the minimal FAM172A promoter, as evaluated using Chromatin immunoprecipitation (ChIP) and Electrophoreticmobility shift assay (EMSA). The results of Western blot analysis and qRT-PCR indicated that STAT1 was able to upregulate the expression of FAM172A. Our results showed that FAM172A could suppress proliferation of colon cancer cells, and STAT1 could bind to the minimum promoter region of FAM172A and upregulated the expression of FAM172A. These results may provide advanced insights into the functions of FAM172A and its regulatory mechanisms.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter

Retraction in: /10.3892/or.2023.8545

This article is mentioned in:

Abstract

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