FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter Retraction in /10.3892/or.2023.8545

Qian,Kai; Zhang,Jinqian; Lu,Jingbo; Liu,Wenjun; Yao,Xingxing; Chen,Qing; Lu,Shun; Xiang,Guoan; Liu,Hao

doi:10.3892/or.2015.4485

FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter

Retraction in: /10.3892/or.2023.8545

Authors:
- Kai Qian
- Jinqian Zhang
- Jingbo Lu
- Wenjun Liu
- Xingxing Yao
- Qing Chen
- Shun Lu
- Guoan Xiang
- Hao Liu
View Affiliations

Affiliations: Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Laboratory Medicine, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China, Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China
Published online on: December 10, 2015 https://doi.org/10.3892/or.2015.4485
Pages: 1273-1280
Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In our previous study, low expression of FAM172A protein was found in colon cancer tissues. This research was planned to explore the functions of FAM172A gene and examine the mechanisms of its transcriptional regulation. Firstly, flow cytometry showed that FAM172A inhibited proliferation and promoted apoptosis and differentiation of colon cancer cells. Then through continuous truncation, we identified the minimal functional promoter region of FAM172A. Subsequently, we found that STAT1, as a transcription factor, could bind to the minimal FAM172A promoter, as evaluated using Chromatin immunoprecipitation (ChIP) and Electrophoreticmobility shift assay (EMSA). The results of Western blot analysis and qRT-PCR indicated that STAT1 was able to upregulate the expression of FAM172A. Our results showed that FAM172A could suppress proliferation of colon cancer cells, and STAT1 could bind to the minimum promoter region of FAM172A and upregulated the expression of FAM172A. These results may provide advanced insights into the functions of FAM172A and its regulatory mechanisms.

View Figures

View References

1	Yu CS, Chen YC, Lu CH and Hwang JK: Prediction of protein subcellular localization. Proteins. 64:643–651. 2006. View Article : Google Scholar : PubMed/NCBI
2	Li L, Dong X, Leong MC, Zhou W, Yang Z, Chen F, Bao Y, Jia W and Hu R: Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells. Int J Mol Med. 26:483–490. 2010.PubMed/NCBI
3	Li LX, Tao Z, Dong XH, Liang WC, Yang Zh, Mou B, Bao YQ, Wang C, Jia WP and Hu RM: Molecular cloning of a novel gene, C5orf21 gene and its roles in diabetic macroangiopathy. Zhonghua Yi Xue Za Zhi. 89:2574–2577. 2009.in Chinese.
4	Feng Z, Li H, Liu S, Cheng J, Xiang G and Zhang J: FAM172A induces S phase arrest of HepG2 cells via Notch 3. Oncol Rep. 29:1154–1160. 2013.PubMed/NCBI
5	Ehebauer M, Hayward P and Arias AM: Notch, a universal arbiter of cell fate decisions. Science. 314:1414–1415. 2006. View Article : Google Scholar : PubMed/NCBI
6	DeVries TA, Kalkofen RL, Matassa AA and Reyland ME: Protein kinase Cdelta regulates apoptosis via activation of STAT1. J Biol Chem. 279:45603–45612. 2004. View Article : Google Scholar : PubMed/NCBI
7	Heim MH: The Jak-STAT pathway: Cytokine signalling from the receptor to the nucleus. J Recept Signal Transduct Res. 19:75–120. 1999. View Article : Google Scholar : PubMed/NCBI
8	Rawlings JS, Rosler KM and Harrison DA: The JAK/STAT signaling pathway. J Cell Sci. 117:1281–1283. 2004. View Article : Google Scholar : PubMed/NCBI
9	Kisseleva T, Bhattacharya S, Braunstein J and Schindler CW: Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene. 285:1–24. 2002. View Article : Google Scholar : PubMed/NCBI
10	Agrawal S, Agarwal ML, Chatterjee-Kishore M, Stark GR and Chisolm GM: Stat1-dependent, p53-independent expression of p21(waf1) modulates oxysterol-induced apoptosis. Mol Cell Biol. 22:1981–1992. 2002. View Article : Google Scholar : PubMed/NCBI
11	Townsend PA, Scarabelli TM, Davidson SM, Knight RA, Latchman DS and Stephanou A: STAT-1 interacts with p53 to enhance DNA damage-induced apoptosis. J Biol Chem. 279:5811–5820. 2004. View Article : Google Scholar
12	Dupuis S, Jouanguy E, Al-Hajjar S, Fieschi C, Al-Mohsen IZ, Al-Jumaah S, Yang K, Chapgier A, Eidenschenk C, Eid P, et al: Impaired response to interferon-alpha/beta and lethal viral disease in human STAT1 deficiency. Nat Genet. 33:388–391. 2003. View Article : Google Scholar : PubMed/NCBI
13	Datta M and Bhattacharyya NP: Regulation of RE1 protein silencing transcription factor (REST) expression by HIP1 protein interactor (HIPPI). J Biol Chem. 286:33759–33769. 2011. View Article : Google Scholar : PubMed/NCBI
14	Haybaeck J, Obrist P, Schindler CU, Spizzo G and Doppler W: STAT-1 expression in human glioblastoma and peritumoral tissue. Anticancer Res. 27:3829–3835. 2007.
15	Das S and Bhattacharyya NP: Transcription regulation of HYPK by Heat Shock Factor 1. PLoS One. 9:e855522014. View Article : Google Scholar : PubMed/NCBI
16	Gao X, Wang Q, Li W, Yang B, Song H, Ju W, Liu S and Cheng J: Identification of nucleolar and coiled-body phosphoprotein 1 (NOLC1) minimal promoter regulated by NF-κB and CREB. BMB Rep. 44:70–75. 2011. View Article : Google Scholar : PubMed/NCBI
17	Pradhan SA, Rather MI, Tiwari A, Bhat VK and Kumar A: Evidence that TSC2 acts as a transcription factor and binds to and represses the promoter of Epiregulin. Nucleic Acids Res. 42:6243–6255. 2014. View Article : Google Scholar : PubMed/NCBI
18	Cheon H, Yang J and Stark GR: The functions of signal transducers and activators of transcriptions 1 and 3 as cytokine-inducible proteins. J Interferon Cytokine Res. 31:33–40. 2011. View Article : Google Scholar :
19	Chapgier A, Boisson-Dupuis S, Jouanguy E, Vogt G, Feinberg J, Prochnicka-Chalufour A, Casrouge A, Yang K, Soudais C, Fieschi C, et al: Novel STAT1 alleles in otherwise healthy patients with mycobacterial disease. PLoS Genet. 2:e1312006. View Article : Google Scholar : PubMed/NCBI
20	Stark GR and Darnell JE Jr: The JAK-STAT pathway at twenty. Immunity. 36:503–514. 2012. View Article : Google Scholar : PubMed/NCBI
21	Pfitzner E, Kliem S, Baus D and Litterst CM: The role of STATs in inflammation and inflammatory diseases. Curr Pharm Des. 10:2839–2850. 2004. View Article : Google Scholar : PubMed/NCBI