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Article

MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma

  • Authors:
    • Qianqian Zhang
    • Shixing Zhao
    • Xiaoli Pang
    • Baorong Chi
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Diseases, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Intensive Care Unit, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China
  • Pages: 1831-1840
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    Published online on: December 16, 2015
       https://doi.org/10.3892/or.2015.4491
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Abstract

MicroRNAs (miRs) have emerged as prospective tools for human cancer therapy, including hepatocellular carcinoma (HCC) therapy. Previous studies have suggested that miR-381 functions as oncogenic or tumor-suppressive miRs in other cancer types. However, the role of miR-381 in HCC remains unknown. The present study investigated the expression and functional role of miR-381 in HCC. miR-381 expression was significantly decreased in HCC tissues and cell lines. miR-381 overexpression significantly inhibited HCC cell proliferation and colony formation, induced G0/G1 cell cycle arrest and suppressed cell invasion. Conversely, suppression of miR-381 showed the opposite effect in HCC cells. Bioinformatics analysis and dual-luciferase reporter assay results showed that miR-381 directly targeted the 3'-untranslated region of liver receptor homolog-1 (LRH-1), and quantitative polymerase chain reaction and western blot analysis results showed that miR-381 negatively modulated LRH-1 expression. Data elucidated that miR-381 directly regulated HCC cell growth and invasion, as well as the Wnt signaling pathways, by targeting LRH-1. Clinical tissue detection data revealed an inverse correlation between miR-381 and LRH-1 expression in HCC tissues, further indicating the functional significance of miR-381-LRH-1 in regulating HCC tumorigenesis. The present study indicates that miR-381 may be a novel tumor suppressor that blocks HCC growth and invasion by targeting LRH-1. The results present novel insights into understanding the molecular mechanism underlying HCC tumorigenesis and provide a future direction to the development of therapeutic interventions for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Zhao S, Pang X and Chi B: MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma. Oncol Rep 35: 1831-1840, 2016.
APA
Zhang, Q., Zhao, S., Pang, X., & Chi, B. (2016). MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma. Oncology Reports, 35, 1831-1840. https://doi.org/10.3892/or.2015.4491
MLA
Zhang, Q., Zhao, S., Pang, X., Chi, B."MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma". Oncology Reports 35.3 (2016): 1831-1840.
Chicago
Zhang, Q., Zhao, S., Pang, X., Chi, B."MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma". Oncology Reports 35, no. 3 (2016): 1831-1840. https://doi.org/10.3892/or.2015.4491
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Zhao S, Pang X and Chi B: MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma. Oncol Rep 35: 1831-1840, 2016.
APA
Zhang, Q., Zhao, S., Pang, X., & Chi, B. (2016). MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma. Oncology Reports, 35, 1831-1840. https://doi.org/10.3892/or.2015.4491
MLA
Zhang, Q., Zhao, S., Pang, X., Chi, B."MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma". Oncology Reports 35.3 (2016): 1831-1840.
Chicago
Zhang, Q., Zhao, S., Pang, X., Chi, B."MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma". Oncology Reports 35, no. 3 (2016): 1831-1840. https://doi.org/10.3892/or.2015.4491
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