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Article

Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice

  • Authors:
    • Zeynep Bostanci
    • Ronald P. Mack
    • Laura M. Enomoto
    • Samina Alam
    • Ashley Brown
    • Carola Neumann
    • David I. Soybel
    • Shannon L. Kelleher
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Pennsylvania State University Hershey College of Medicine, Hershey, PA 17033, USA, Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, P.R. China, Department of Pharmacology, University of Pittsburgh, Pittsburgh, PA 15260, USA
  • Pages: 1409-1416
    |
    Published online on: December 22, 2015
       https://doi.org/10.3892/or.2015.4508
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Abstract

Breastfeeding can reduce breast cancer risk; however, unknown factors modify this protective effect. Zinc (Zn) modulates an array of cellular functions including oxidative stress, cell proliferation, motility and apoptosis. Marginal Zn intake is common in women and is associated with breast cancer. We reported that marginal Zn intake in mice leads to mammary gland hypoplasia and hallmarks of pre-neoplastic lesions. In the present study, we tested the hypothesis that marginal Zn intake confounds the protective effect of lactation on breast cancer. Nulliparous mice fed control (ZA, 30 mg Zn/kg) or a marginal Zn diet (ZD, 15 mg Zn/kg), were bred and offspring were weaned naturally. Post-involution, mice were gavaged with corn oil or 7,12-dimethylbenz(a)anthracene (DMBA, 1 mg/wk for 4 weeks) and tumor development was monitored. A ZD diet led to insufficient involution, increased fibrosis and oxidative stress. Following DMBA treatment, mice fed ZD had higher oxidative stress in mammary tissue that correlated with reduced levels of peroxiredoxin-1 and p53 and tended to have shorter tumor latency and greater incidence of non-palpable tumors. In summary, marginal Zn intake creates a toxic mammary gland microenvironment and abrogates the protective effect of lactation on carcinogenesis.
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1 

Collaborative Group on Hormonal Factors in Breast Cancer: Breast cancer and breastfeeding: Collaborative reanalysis of individual data from 47 epidemiological studies in 30 countries, including 50302 women with breast cancer and 96973 women without the disease. Lancet. 360:187–195. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Anothaisintawee T, Wiratkapun C, Lerdsitthichai P, Kasamesup V, Wongwaisayawan S, Srinakarin J, Hirunpat S, Woodtichartpreecha P, Boonlikit S, Teerawattananon Y, et al: Risk factors of breast cancer: a systematic review and meta-analysis. Asia Pac J Public Health. 25:368–387. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Bernier MO, Plu-Bureau G, Bossard N, Ayzac L and Thalabard JC: Breastfeeding and risk of breast cancer: A metaanalysis of published studies. Hum Reprod Update. 6:374–386. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Michels KB, Willett WC, Rosner BA, Manson JE, Hunter DJ, Colditz GA, Hankinson SE and Speizer FE: Prospective assessment of breastfeeding and breast cancer incidence among 89,887 women. Lancet. 347:431–436. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Dempsey C, McCormick NH, Croxford TP, Seo YA, Grider A and Kelleher SL: Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition. J Nutr. 142:655–660. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Bostanci Z, Mack RP Jr, Lee S, Soybel DI and Kelleher SL: Paradoxical zinc toxicity and oxidative stress in the mammary gland during marginal dietary zinc deficiency. Reprod Toxicol. 54:84–92. 2015. View Article : Google Scholar

7 

Stang J, Story MT, Harnack L and Neumark-Sztainer D: Relationships between vitamin and mineral supplemnt use, dietary intake, and dietary adequacy among adolescents. J Am Diet Assoc. 100:905–910. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Herbst CA, Menon KC, Ferguson EL, Thomson CD, Bailey K, Gray AR, Zodpey S, Saraf A, Das PK and Skeaff SA: Dietary and non-dietary factors associated with serum zinc in Indian women. Biol Trace Elem Res. 161:38–47. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Scheplyagina LA: Impact of the mother's zinc deficiency on the woman's and newborn's health status. J Trace Elem Med Biol. 19:29–35. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Gumulec J, Masarik M, Adam V, Eckschlager T, Provaznik I and Kizek R: Serum and tissue zinc in epithelial malignancies: A meta-analysis. PLoS One. 9:e997902014. View Article : Google Scholar : PubMed/NCBI

11 

Tinoco-Veras CM, Bezerra Sousa MS, da Silva BB, Franciscato Cozzolino SM, Viana Pires L, Coelho Pimentel JA, do Nascimento-Nogueira N and do Nascimento-Marreiro D: Analysis of plasma and erythrocyte zinc levels in premenopausal women with breast cancer. Nutr Hosp. 26:293–297. 2011.PubMed/NCBI

12 

Geraki K, Farquharson MJ and Bradley DA: Concentrations of Fe, Cu and Zn in breast tissue: A synchrotron XRF study. Phys Med Biol. 47:2327–2339. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Lopez V, Foolad F and Kelleher SL: ZnT2-overexpression represses the cytotoxic effects of zinc hyper-accumulation in malignant metallothionein-null T47D breast tumor cells. Cancer Lett. 304:41–51. 2011. View Article : Google Scholar : PubMed/NCBI

14 

McCormick NH, Hennigar SR, Kiselyov K and Kelleher SL: The biology of zinc transport in mammary epithelial cells: Implications for mammary gland development, lactation, and involution. J Mammary Gland Biol Neoplasia. 19:59–71. 2014. View Article : Google Scholar

15 

Jezierska-Drutel A, Rosenzweig SA and Neumann CA: Role of oxidative stress and the microenvironment in breast cancer development and progression. Adv Cancer Res. 119:107–125. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Formigari A, Gregianin E and Irato P: The effect of zinc and the role of p53 in copper-induced cellular stress responses. J Appl Toxicol. 33:527–536. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Eide DJ: The oxidative stress of zinc deficiency. Metallomics. 3:1124–1129. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Hadler-Olsen E, Winberg JO and Uhlin-Hansen L: Matrix metal-loproteinases in cancer: their value as diagnostic and prognostic markers and therapeutic targets. Tumour Biol. 34:2041–2051. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Grattan BJ and Freake HC: Zinc and cancer: Implications for LIV-1 in breast cancer. Nutrients. 4:648–675. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Lopez V and Kelleher SL: Zip6-attenuation promotes epithelial-to-mesenchymal transition in ductal breast tumor (T47D) cells. Exp Cell Res. 316:366–375. 2010. View Article : Google Scholar

21 

Shen H, Qin H and Guo J: Concordant correlation of LIV-1 and E-cadherin expression in human breast cancer cell MCF-7. Mol Biol Rep. 36:653–659. 2009. View Article : Google Scholar

22 

Sussman D, Smith LM, Anderson ME, Duniho S, Hunter JH, Kostner H, Miyamoto JB, Nesterova A, Westendorf L, Van Epps HA, et al: SGN-LIV1A: A novel antibody-drug conjugate targeting LIV-1 for the treatment of metastatic breast cancer. Mol Cancer Ther. 13:2991–3000. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Bostanci Z, Alam S, Soybel DI and Kelleher SL: Prolactin receptor attenuation induces zinc pool redistribution through ZnT2 and decreases invasion in MDA-MB-453 breast cancer cells. Exp Cell Res. 321:190–200. 2014. View Article : Google Scholar

24 

Chowanadisai W, Lönnerdal B and Kelleher SL: Zip6 (LIV-1) regulates zinc uptake in neuroblastoma cells under resting but not depolarizing conditions. Brain Res. 1199:10–19. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Takatani-Nakase T, Matsui C, Maeda S, Kawahara S and Takahashi K: High glucose level promotes migration behavior of breast cancer cells through zinc and its transporters. PLoS One. 9:e901362014. View Article : Google Scholar : PubMed/NCBI

26 

Hogstrand C, Kille P, Ackland ML, Hiscox S and Taylor KM: A mechanism for epithelial-mesenchymal transition and anoikis resistance in breast cancer triggered by zinc channel ZIP6 and STAT3 (signal transducer and activator of transcription 3). Biochem J. 455:229–237. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Kasper G, Weiser AA, Rump A, Sparbier K, Dahl E, Hartmann A, Wild P, Schwidetzky U, Castaños-Vélez E, Lehmann K, et al: Expression levels of the putative zinc transporter LIV-1 are associated with a better outcome of breast cancer patients. Int J Cancer. 117:961–973. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Palmiter RD, Cole TB and Findley SD: ZnT-2, a mammalian protein that confers resistance to zinc by facilitating vesicular sequestration. EMBO J. 15:1784–1791. 1996.PubMed/NCBI

29 

Yang J, Yoshizawa K, Nandi S and Tsubura A: Protective effects of pregnancy and lactation against N-methyl-N-nitrosourea-induced mammary carcinomas in female Lewis rats. Carcinogenesis. 20:623–628. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Croxford TP, McCormick NH and Kelleher SL: Moderate zinc deficiency reduces testicular Zip6 and Zip10 abundance and impairs spermatogenesis in mice. J Nutr. 141:359–365. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Russo J and Russo IH: Susceptibility of the mammary gland to carcinogenesis. II. Pregnancy interruption as a risk factor in tumor incidence. Am J Pathol. 100:497–512. 1980.PubMed/NCBI

32 

Varin A, Larbi A, Dedoussis GV, Kanoni S, Jajte J, Rink L, Monti D, Malavolta M, Marcellini F, Mocchegiani E, et al: In vitro and in vivo effects of zinc on cytokine signalling in human T cells. Exp Gerontol. 43:472–482. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Hennigar SR, Seo YA, Sharma S, Soybel DI and Kelleher SL: ZnT2 is a critical mediator of lysosomal-mediated cell death during early mammary gland involution. Sci Rep. 5:80332015. View Article : Google Scholar : PubMed/NCBI

34 

Haricharan S and Li Y: STAT signaling in mammary gland differentiation, cell survival and tumorigenesis. Mol Cell Endocrinol. 382:560–569. 2014. View Article : Google Scholar

35 

Patel II, Shearer DA, Fogarty SW, Fullwood NJ, Quaroni L, Martin FL and Weisz J: Infrared microspectroscopy identifies biomolecular changes associated with chronic oxidative stress in mammary epithelium and stroma of breast tissues from healthy young women: Implications for latent stages of breast carcinogenesis. Cancer Biol Ther. 15:225–235. 2014. View Article : Google Scholar :

36 

MacDiarmid CW, Taggart J, Kerdsomboon K, Kubisiak M, Panascharoen S, Schelble K and Eide DJ: Peroxiredoxin chaperone activity is critical for protein homeostasis in zinc-deficient yeast. J Biol Chem. 288:31313–31327. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Provenzano PP, Inman DR, Eliceiri KW, Knittel JG, Yan L, Rueden CT, White JG and Keely PJ: Collagen density promotes mammary tumor initiation and progression. BMC Med. 6:112008. View Article : Google Scholar : PubMed/NCBI

38 

Allen RG and Tresini M: Oxidative stress and gene regulation. Free Radic Biol Med. 28:463–499. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bostanci Z, Mack RP, Enomoto LM, Alam S, Brown A, Neumann C, Soybel DI and Kelleher SL: Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice. Oncol Rep 35: 1409-1416, 2016.
APA
Bostanci, Z., Mack, R.P., Enomoto, L.M., Alam, S., Brown, A., Neumann, C. ... Kelleher, S.L. (2016). Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice. Oncology Reports, 35, 1409-1416. https://doi.org/10.3892/or.2015.4508
MLA
Bostanci, Z., Mack, R. P., Enomoto, L. M., Alam, S., Brown, A., Neumann, C., Soybel, D. I., Kelleher, S. L."Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice". Oncology Reports 35.3 (2016): 1409-1416.
Chicago
Bostanci, Z., Mack, R. P., Enomoto, L. M., Alam, S., Brown, A., Neumann, C., Soybel, D. I., Kelleher, S. L."Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice". Oncology Reports 35, no. 3 (2016): 1409-1416. https://doi.org/10.3892/or.2015.4508
Copy and paste a formatted citation
x
Spandidos Publications style
Bostanci Z, Mack RP, Enomoto LM, Alam S, Brown A, Neumann C, Soybel DI and Kelleher SL: Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice. Oncol Rep 35: 1409-1416, 2016.
APA
Bostanci, Z., Mack, R.P., Enomoto, L.M., Alam, S., Brown, A., Neumann, C. ... Kelleher, S.L. (2016). Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice. Oncology Reports, 35, 1409-1416. https://doi.org/10.3892/or.2015.4508
MLA
Bostanci, Z., Mack, R. P., Enomoto, L. M., Alam, S., Brown, A., Neumann, C., Soybel, D. I., Kelleher, S. L."Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice". Oncology Reports 35.3 (2016): 1409-1416.
Chicago
Bostanci, Z., Mack, R. P., Enomoto, L. M., Alam, S., Brown, A., Neumann, C., Soybel, D. I., Kelleher, S. L."Marginal zinc intake reduces the protective effect of lactation on mammary gland carcinogenesis in a DMBA-induced tumor model in mice". Oncology Reports 35, no. 3 (2016): 1409-1416. https://doi.org/10.3892/or.2015.4508
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