Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March-2016 Volume 35 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2016 Volume 35 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro

  • Authors:
    • Ruimin Wu
    • Yijia Chen
    • Fuyan Li
    • Wei Li
    • Hong Zhou
    • Yi Yang
    • Zhijun Pei
  • View Affiliations / Copyright

    Affiliations: Department of PET Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 1750-1756
    |
    Published online on: December 23, 2015
       https://doi.org/10.3892/or.2015.4512
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The purpose of the present study was to explore the role and mechanism of extracellular ecto-5'-nucleotidase (CD73) in human colorectal cancer growth. Firstly, CD73 expression was detected in colorectal cancer cell lines both at the mRNA and protein levels. Secondly, recombinant CD73 interference and overexpression lentiviruses were used, respectively. Colony formation assay, CCK-8 assay and flow cytometry were used to investigate the impact of CD73 on colorectal cancer cell proliferation and cell cycle distribution. Then, adenosine and CD73 enzyme activity inhibitor (APCP) were used to study the effect of CD73 on Epidermal growth factor receptor (EGFR) and β-catenin/cyclin D1 signaling pathways. Finally, a human colorectal cancer transplantation nude mouse model was used to observe the effect of CD73 on tumor growth in vivo. As the results showed, CD73 was highly expressed in the colorectal cancer cell lines. CD73 promoted colorectal cancer cell proliferation both in vivo and in vitro. CD73 activated EGFR and the β-catenin/cyclin D1 signaling pathways through its enzyme and non-enzyme activities. All of the results confirmed that CD73 promotes the growth of human colorectal cancer cells through EGFR and the β-catenin/cyclin D1 signaling pathway. CD73 may be used as a valuable biomarker of colorectal cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Brody H: Colorectal cancer. Nature. 521:S12015. View Article : Google Scholar : PubMed/NCBI

2 

Paulson EC, Veenstra CM, Vachani A, Ciunci CA and Epstein AJ: Trends in surveillance for resected colorectal cancer, 2001–2009. Cancer. 121:3525–3533. 2015. View Article : Google Scholar : PubMed/NCBI

3 

van Vugt JL, Reisinger KW, Derikx JP, Boerma D and Stoot JH: Improving the outcomes in oncological colorectal surgery. World J Gastroenterol. 20:12445–12457. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Prorokov VV, Vlasov OA and Tupitsyn NN: Current state of the problem of treatment and prognosis of colorectal cancer. Vopr Onkol. 60:28–33. 2014.In Russian.

5 

Li L and Ma BB: Colorectal cancer in Chinese patients: Current and emerging treatment options. Onco Targets Ther. 7:1817–1828. 2014.PubMed/NCBI

6 

Vignali A and De Nardi P: Multidisciplinary treatment of rectal cancer in 2014: Where are we going? World J Gastroenterol. 20:11249–11261. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Binefa G, Rodríguez-Moranta F, Teule A and Medina-Hayas M: Colorectal cancer: From prevention to personalized medicine. World J Gastroenterol. 20:6786–6808. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Ahmed S, Johnson K, Ahmed O and Iqbal N: Advances in the management of colorectal cancer: From biology to treatment. Int J Colorectal Dis. 29:1031–1042. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Morris V and Kopetz S: Clinical biomarkers in colorectal cancer. Clin Adv Hematol Oncol. 11:768–776. 2013.

10 

Chen Y, Thelin WR, Yang B, Milgram SL and Jacobson K: Transient anchorage of cross-linked glycosyl-phosphatidylinositol-anchored proteins depends on cholesterol, Src family kinases, caveolin, and phosphoinositides. J Cell Biol. 175:169–178. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Liu N, Fang XD and Vadis Q: CD73 as a novel prognostic biomarker for human colorectal cancer. J Surg Oncol. 106:918–920. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Zhi X, Wang Y, Zhou X, Yu J, Jian R, Tang S, Yin L and Zhou P: RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells. Cancer Sci. 101:2561–2569. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Gao ZW, Dong K and Zhang HZ: The roles of CD73 in cancer. Biomed Res Int. 2014:4606542014. View Article : Google Scholar : PubMed/NCBI

14 

Häusler SF, Montalbán del Barrio I, Strohschein J, Chandran PA, Engel JB, Hönig A, Ossadnik M, Horn E, Fischer B, Krockenberger M, et al: Ectonucleotidases CD39 and CD73 on OvCA cells are potent adenosine-generating enzymes responsible for adenosine receptor 2A-dependent suppression of T cell function and NK cell cytotoxicity. Cancer Immunol Immunother. 60:1405–1418. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Stagg J, Divisekera U, McLaughlin N, Sharkey J, Pommey S, Denoyer D, Dwyer KM and Smyth MJ: Anti-CD73 antibody therapy inhibits breast tumor growth and metastasis. Proc Natl Acad Sci USA. 107:1547–1552. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Beavis PA, Stagg J, Darcy PK and Smyth MJ: CD73: A potent suppressor of antitumor immune responses. Trends Immunol. 33:231–237. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Stagg J, Beavis PA, Divisekera U, Liu MC, Möller A, Darcy PK and Smyth MJ: CD73-deficient mice are resistant to carcinogenesis. Cancer Res. 72:2190–2196. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Zhang B: CD73 promotes tumor growth and metastasis. Oncoimmunology. 1:67–70. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Resta R and Thompson LF: T cell signalling through CD73. Cell Signal. 9:131–139. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Jin D, Fan J, Wang L, Thompson LF, Liu A, Daniel BJ, Shin T, Curiel TJ and Zhang B: CD73 on tumor cells impairs antitumor T-cell responses: A novel mechanism of tumor-induced immune suppression. Cancer Res. 70:2245–2255. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Knapp K, Zebisch M, Pippel J, El-Tayeb A, Müller CE and Sträter N: Crystal structure of the human ecto-5′-nucleotidase (CD73): Insights into the regulation of purinergic signaling. Structure. 20:2161–2173. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Deaglio S and Robson SC: Ectonucleotidases as regulators of purinergic signaling in thrombosis, inflammation, and immunity. Adv Pharmacol. 61:301–332. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Young A, Mittal D, Stagg J and Smyth MJ: Targeting cancer-derived adenosine: New therapeutic approaches. Cancer Discov. 4:879–888. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Lee MS and Kopetz S: Current and future approaches to target the epidermal growth factor receptor and its downstream signaling in metastatic colorectal cancer. Clin Colorectal Cancer. 14:203–218. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Pietrantonio F, Petrelli F, Coinu A, Di Bartolomeo M, Borgonovo K, Maggi C, Cabiddu M, Iacovelli R, Bossi I, Lonati V, et al: Predictive role of BRAF mutations in patients with advanced colorectal cancer receiving cetuximab and panitumumab: A meta-analysis. Eur J Cancer. 51:587–594. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Krawczyk PA and Kowalski DM: Genetic and immune factors underlying the efficacy of cetuximab and panitumumab in the treatment of patients with metastatic colorectal cancer. Contemp Oncol (Pozn). 18:7–16. 2014.

27 

Coget J, Borrini F, Susman S and Sabourin JC: Colorectal carcinomas in 2013: The search for powerful prognostic markers is still on the go! Cancer Biomark. 14:145–150. 2014.PubMed/NCBI

28 

Sideris M and Papagrigoriadis S: Molecular biomarkers and classification models in the evaluation of the prognosis of colorectal cancer. Anticancer Res. 34:2061–2068. 2014.PubMed/NCBI

29 

Häusler SF, Del Barrio IM, Diessner J, Stein RG, Strohschein J, Hönig A, Dietl J and Wischhusen J: Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion. Am J Transl Res. 6:129–139. 2014.PubMed/NCBI

30 

Wu XR, He XS, Chen YF, Yuan RX, Zeng Y, Lian L, Zou YF, Lan N, Wu XJ and Lan P: High expression of CD73 as a poor prognostic biomarker in human colorectal cancer. J Surg Oncol. 106:130–137. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Yang Q, Du J and Zu L: Overexpression of CD73 in prostate cancer is associated with lymph node metastasis. Pathol Oncol Res. 19:811–814. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Wang LL, Tang HP, Shi GC, Wan HY, Tang W, Hou XX, Pan LN, Shi BY and Tao LQ: CD39/CD73 and the imbalance of Th17 cells and regulatory T cells in allergic asthma. Mol Med Rep. 8:1432–1438. 2013.PubMed/NCBI

33 

Smyth LA, Ratnasothy K, Tsang JY, Boardman D, Warley A, Lechler R and Lombardi G: CD73 expression on extracellular vesicles derived from CD4+ CD25+ Foxp3+ T cells contributes to their regulatory function. Eur J Immunol. 43:2430–2440. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Zhi X, Wang Y, Yu J, Yu J, Zhang L, Yin L and Zhou P: Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer. IUBMB Life. 64:911–920. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Zhou X, Zhi X, Zhou P, Chen S, Zhao F, Shao Z, Ou Z and Yin L: Effects of ecto-5′-nucleotidase on human breast cancer cell growth in vitro and in vivo. Oncol Rep. 17:1341–1346. 2007.PubMed/NCBI

36 

Zhi X, Chen S, Zhou P, Shao Z, Wang L, Ou Z and Yin L: RNA interference of ecto-5′-nucleotidase (CD73) inhibits human breast cancer cell growth and invasion. Clin Exp Metastasis. 24:439–448. 2007. View Article : Google Scholar

37 

Ring A, Kim YM and Kahn M: Wnt/catenin signaling in adult stem cell physiology and disease. Stem Cell Rev. 10:512–525. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Lee JK, Park SR, Jung BK, Jeon YK, Lee YS, Kim MK, Kim YG, Jang JY and Kim CW: Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells. PLoS One. 8:e842562013. View Article : Google Scholar

39 

Gopalakrishnan N, Saravanakumar M, Madankumar P, Thiyagu M and Devaraj H: Colocalization of β-catenin with Notch intracellular domain in colon cancer: A possible role of Notch1 signaling in activation of cyclinD1-mediated cell proliferation. Mol Cell Biochem. 396:281–293. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Horst D: Plasticity of WNT signaling activity in colorectal cancer. Pathologe. 33(Suppl 2): 194–197. 2012.In German. View Article : Google Scholar

41 

Sebio A, Kahn M and Lenz HJ: The potential of targeting Wnt/β-catenin in colon cancer. Expert Opin Ther Targets. 18:611–615. 2014. View Article : Google Scholar : PubMed/NCBI

42 

Wang L, Tang S, Wang Y, Xu S, Yu J, Zhi X, Ou Z, Yang J, Zhou P and Shao Z: Ecto-5′-nucleotidase (CD73) promotes tumor angiogenesis. Clin Exp Metastasis. 30:671–680. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Wang L, Zhou X, Zhou T, Ma D, Chen S, Zhi X, Yin L, Shao Z, Ou Z and Zhou P: Ecto-5′-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells. J Cancer Res Clin Oncol. 134:365–372. 2008. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu R, Chen Y, Li F, Li W, Zhou H, Yang Y and Pei Z: Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro. Oncol Rep 35: 1750-1756, 2016.
APA
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., & Pei, Z. (2016). Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro. Oncology Reports, 35, 1750-1756. https://doi.org/10.3892/or.2015.4512
MLA
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., Pei, Z."Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro". Oncology Reports 35.3 (2016): 1750-1756.
Chicago
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., Pei, Z."Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro". Oncology Reports 35, no. 3 (2016): 1750-1756. https://doi.org/10.3892/or.2015.4512
Copy and paste a formatted citation
x
Spandidos Publications style
Wu R, Chen Y, Li F, Li W, Zhou H, Yang Y and Pei Z: Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro. Oncol Rep 35: 1750-1756, 2016.
APA
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., & Pei, Z. (2016). Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro. Oncology Reports, 35, 1750-1756. https://doi.org/10.3892/or.2015.4512
MLA
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., Pei, Z."Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro". Oncology Reports 35.3 (2016): 1750-1756.
Chicago
Wu, R., Chen, Y., Li, F., Li, W., Zhou, H., Yang, Y., Pei, Z."Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro". Oncology Reports 35, no. 3 (2016): 1750-1756. https://doi.org/10.3892/or.2015.4512
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team