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Article

Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation

  • Authors:
    • Yinli Yang
    • Cuiping Li
    • Yun Fu
    • Youxun Liu
    • Yu Zhang
    • Yanfang Zhang
    • Pingxin Zhou
    • Yanbin Yuan
    • Sufeng Zhou
    • Shaoshan Li
    • Changzheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Biochemistry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Surgery, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 1636-1644
    |
    Published online on: December 29, 2015
       https://doi.org/10.3892/or.2015.4530
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Abstract

Many anticancer drugs used in the clinical have potent metal chelating ability. The formed metal complex(es) may exhibit improved (or antagonistic) antitumor activity. However, the underlying mechanism has received limited attention. Therefore, investigation of the mechanism involved in the change upon chelation is required to extend our understanding of the effects of various drugs. In the present study, the proliferation inhibition effect of benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone (BNMPH) and its copper complex on tumor cell lines was investigated. The copper chelate exhibited almost a 10-fold increase in antitumor activity (with IC50 <5 µM). The results showed that both BNMPH and its copper complex induced reactive oxygen species (ROS) generation, and caused upregulation of caspase 8 and Bax as well as the downregulation of Bcl-2, indicating that apoptosis was involved in the cytotoxic effects. DNA fragmentation noted in the comet assay further supported ROS involvement. The present study indicated that BNMPH and its copper complex effectively induced S phase arrest and the cell cycle arrest was associated with the downregulation of cyclin D1. The formation of acidic vesicular organelles (AVOs) and an increase in cleaved LC3-II demonstrated that autophagy occurred in the HepG2 cells treated with the agents. Taken together, BNMPH and its copper complex exhibited proliferation inhibition via apoptosis, cell cycle arrest and autophagy, which was dependent on ROS. The enhanced antitumor activity of the copper complex was due to its redox-cycling ability, but the mechanism was not altered compared to BNMPH. Our findings may significantly contribute to the understanding of the anti-proliferative effect of BNMPH and its copper complex.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Li C, Fu Y, Liu Y, Zhang Y, Zhang Y, Zhou P, Yuan Y, Zhou S, Li S, Li S, et al: Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation. Oncol Rep 35: 1636-1644, 2016.
APA
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y. ... Li, C. (2016). Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation. Oncology Reports, 35, 1636-1644. https://doi.org/10.3892/or.2015.4530
MLA
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y., Zhou, P., Yuan, Y., Zhou, S., Li, S., Li, C."Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation". Oncology Reports 35.3 (2016): 1636-1644.
Chicago
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y., Zhou, P., Yuan, Y., Zhou, S., Li, S., Li, C."Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation". Oncology Reports 35, no. 3 (2016): 1636-1644. https://doi.org/10.3892/or.2015.4530
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Li C, Fu Y, Liu Y, Zhang Y, Zhang Y, Zhou P, Yuan Y, Zhou S, Li S, Li S, et al: Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation. Oncol Rep 35: 1636-1644, 2016.
APA
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y. ... Li, C. (2016). Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation. Oncology Reports, 35, 1636-1644. https://doi.org/10.3892/or.2015.4530
MLA
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y., Zhou, P., Yuan, Y., Zhou, S., Li, S., Li, C."Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation". Oncology Reports 35.3 (2016): 1636-1644.
Chicago
Yang, Y., Li, C., Fu, Y., Liu, Y., Zhang, Y., Zhang, Y., Zhou, P., Yuan, Y., Zhou, S., Li, S., Li, C."Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation". Oncology Reports 35, no. 3 (2016): 1636-1644. https://doi.org/10.3892/or.2015.4530
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