Complex analysis of the p53 tumor suppressor in lung carcinoma

Smardova,Jana; Liskova,Kvetoslava; Ravcukova,Barbora; Malcikova,Jitka; Hausnerova,Jitka; Svitakova,Miluse; Hrabalkova,Renata; Zlamalikova,Lenka; Stano-Kozubik,Katerina; Blahakova,Ivona; Speldova,Jana; Jarkovsky,Jiri; Smarda,Jan

doi:10.3892/or.2015.4533

March-2016 Volume 35 Issue 3

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March-2016 Volume 35 Issue 3

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Article

Complex analysis of the p53 tumor suppressor in lung carcinoma

Authors:
- Jana Smardova
- Kvetoslava Liskova
- Barbora Ravcukova
- Jitka Malcikova
- Jitka Hausnerova
- Miluse Svitakova
- Renata Hrabalkova
- Lenka Zlamalikova
- Katerina Stano-Kozubik
- Ivona Blahakova
- Jana Speldova
- Jiri Jarkovsky
- Jan Smarda
View Affiliations / Copyright

Affiliations: Department of Pathology, University Hospital, Brno, Czech Republic, Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic, Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic, Department of Respiratory Diseases and TB, University Hospital, Brno, Czech Republic, Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Pages: 1859-1867
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Published online on: December 29, 2015

https://doi.org/10.3892/or.2015.4533
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Abstract

Lung cancer is the leading cause of cancer-related deaths worldwide. The p53 tumor suppressor is a transcription factor controlling expression of its target genes in response to various stress stimuli. Mutations of the TP53 gene occur very frequently in lung carcinomas and they play an important role in both oncogenic transformation of lung epithelial cells and lung carcinoma progression. We determined the TP53 status in 42 samples of squamous cell lung carcinoma (SQCC) and 56 samples of lung adenocarcinoma (AC) by the functional analysis FASAY and its variant called split assay. Altogether, we detected 64 TP53 mutations in 63 patients and analyzed them by cDNA and gDNA sequencing. The TP53 mutations were found in 76.2% (32/42) of SQCC cases, and 55.4% (31/56) of ACs. Immunoblotting revealed the p53 protein accumulation in 18 samples (42.9%) among SQCC cases and 19 samples (33.9%) among AC cases. Using fluorescence in situ hybridization we detected loss of the TP53-specific 17p13.3 locus in 23 from 41 analyzed SQCC samples (56.1%) and in 20 from 54 analyzed AC samples (37.0%). We did not find any statistically significant differences in overall and disease-free survival in relation to TP53 status.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Complex analysis of the p53 tumor suppressor in lung carcinoma

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