miR-17-5p promotes the growth of osteosarcoma in a BRCC2-dependent mechanism Retraction in /10.3892/or.2024.8710

Wang,Wei; Zhang,Liang; Zheng,Ke; Zhang,Xiaojing

doi:10.3892/or.2016.4542

March-2016 Volume 35 Issue 3

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March-2016 Volume 35 Issue 3

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Article

miR-17-5p promotes the growth of osteosarcoma in a BRCC2-dependent mechanism

Retraction in: /10.3892/or.2024.8710

Authors:
- Wei Wang
- Liang Zhang
- Ke Zheng
- Xiaojing Zhang
View Affiliations / Copyright

Affiliations: Department of Bone Soft Surgery, Liaoning Provincial Tumor Hospital, Shenyang, Liaoning 110042, P.R. China, Department of Thoracic Surgery, Liaoning Provincial Tumor Hospital, Shenyang, Liaoning 110042, P.R. China
Pages: 1473-1482
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Published online on: January 5, 2016

https://doi.org/10.3892/or.2016.4542
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Abstract

MicroRNA-17-5p has been proven upregulated in many human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma is still unclear. Thus, the aim of the present study was to explore the effects of miR-17-5p in osteosarcoma tumorigenesis and development. The expression level of miR-17-5p was quantified by quantitative real-time reverse-transcriptase-polymerase chain reaction in primary osteosarcoma tissues and osteosarcoma cell lines. MTT, Transwell and matrigel assays were used to test the proliferation, migration and invasion of miR-17-5p transfection osteosarcoma cells, and a mouse model was used to investigate tumorigenesis. The expression levels of miR-17-5p in osteosarcoma tissues were significantly higher than those in corresponding non-cancerous bone tissues. In addition, miR-17-5p upregulation more frequently occurred in osteosarcoma specimens with advanced clinical stage, positive distant metastasis and poor response to neo-adjuvant chemotherapy. After miR-17-5p transfection, cell proliferation, migration, invasion and tumorigenesis in the osteosarcoma cells were significantly promoted. We further demonstrated that BRCC2 is a direct target of miR-17-5p. These findings indicate that miR-17-5p may act not only as a novel diagnostic and prognostic marker, but also as a potential target for molecular therapy of osteosarcoma.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

miR-17-5p promotes the growth of osteosarcoma in a BRCC2-dependent mechanism

Retraction in: /10.3892/or.2024.8710

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