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Article

MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells

  • Authors:
    • Rong Gui
    • Rong Huang
    • Jun-Hua Zhang
    • Xian-Hui Wen
    • Xin-Min Nie
  • View Affiliations / Copyright

    Affiliations: The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 2216-2222
    |
    Published online on: January 12, 2016
       https://doi.org/10.3892/or.2016.4550
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Abstract

VEGF induces deterioration of hepatocellular carcinoma (HCC) by enhancing cell proliferation and migration. MicroRNAs regulate many cellular processes. In this study, we examined the regulation of tumorigenesis in HCC cells by microRNAs in relation to the effect of VEGF. Differences in microRNA expression between HepG2 and THLE-3 cells were characterized by microarray analysis. The results showed that miR-199a-5p expression was markedly downregulated in HepG2 cells and was inhibited in VEGF-overexpressing HepG2 cells in a dose- and time-dependent manner. This miRNA also inhibited cell proliferation and migration, as demonstrated by MTT and cell migration assays. Oxidored-nitro domain-containing protein 1 (NOR1), a nitroreductase, was identified as a downstream target gene of miR-199a-5p, and upregulation of NOR1 proved critical for the inhibition of VEGF-induced cell proliferation and migration in HepG2 cells by miR-199a-5p. These results indicate that miR-199a-5p is critical for regulating cell proliferation and migration by targeting and upregulating NOR1 in human HepG2 cells.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Su ZX, Zhao J, Rong ZH, Geng WM, Wu YG and Qin CK: Upregulation of microRNA-25 associates with prognosis in hepatocellular carcinoma. Diagn Pathol. 9:472014. View Article : Google Scholar : PubMed/NCBI

3 

Li S, Dong P, Wang J, Zhang J, Gu J, Wu X, Wu W, Fei X, Zhang Z, Wang Y, et al: Icariin, a natural flavonol glycoside, induces apoptosis in human hepatoma SMMC-7721 cells via a ROS/JNK-dependent mitochondrial pathway. Cancer Lett. 298:222–230. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zhan P, Qian Q and Yu LK: Serum VEGF level is associated with the outcome of patients with hepatocellular carcinoma: A meta-analysis. Hepatobiliary Surg Nutr. 2:209–215. 2013.

5 

Cao X, Zhang L, Shi Y, Sun Y, Dai S, Guo C, Zhu F, Wang Q, Wang J, Wang X, et al: Human tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 suppresses hepatocellular carcinoma metastasis through inhibiting Rac1. Mol Cancer. 12:1492013. View Article : Google Scholar : PubMed/NCBI

6 

Miyahara K, Nouso K, Morimoto Y, Takeuchi Y, Hagihara H, Kuwaki K, Onishi H, Ikeda F, Miyake Y, Nakamura S, et al Okayama Liver Cancer Group: Pro-angiogenic cytokines for prediction of outcomes in patients with advanced hepatocellular carcinoma. Br J Cancer. 109:2072–2078. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Duong T, Koltowska K, Pichol-Thievend C, Le Guen L, Fontaine F, Smith KA, Truong V, Skoczylas R, Stacker SA, Achen MG, et al: VEGFD regulates blood vascular development by modulating SOX18 activity. Blood. 123:1102–1112. 2014. View Article : Google Scholar

8 

Ryzhov S, Biktasova A, Goldstein AE, Zhang Q, Biaggioni I, Dikov MM and Feoktistov I: Role of JunB in adenosine A2B receptor-mediated vascular endothelial growth factor production. Mol Pharmacol. 85:62–73. 2014. View Article : Google Scholar :

9 

Yao HC, Liu T, Meng XY, Han QF, Zhang M and Wang LX: Effect of basic fibroblast growth factor on the myocardial expression of hypoxia-inducible factor-1α and vascular endothelial growth factor following acute myocardial infarction. Heart Lung Circ. 22:946–951. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Cui QT, Li Y, Duan CH, Zhang W and Guo XL: Further evidence for the contribution of the vascular endothelial growth factor gene in coronary artery disease susceptibility. Gene. 521:217–221. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Winnik S, Lohmann C, Siciliani G, von Lukowicz T, Kuschnerus K, Kraenkel N, Brokopp CE, Enseleit F, Michels S, Ruschitzka F, et al: Systemic VEGF inhibition accelerates experimental atherosclerosis and disrupts endothelial homeostasis - implications for cardiovascular safety. Int J Cardiol. 168:2453–2461. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Lo TF, Tsai WC and Chen ST: MicroRNA-21-3p, a berberine-induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth. PLoS One. 8:e756282013. View Article : Google Scholar : PubMed/NCBI

14 

Li BA: A novel tumor suppressor miRNA miR-520e contributes to suppression of hepatoma. Acta Pharmacol Sin. 33:3–4. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Iorio MV and Croce CM: microRNA involvement in human cancer. Carcinogenesis. 33:1126–1133. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Shi XE, Li YF, Jia L, Ji HL, Song ZY, Cheng J, Wu GF, Song CC, Zhang QL, Zhu JY, et al: MicroRNA-199a-5p affects porcine preadipocyte proliferation and differentiation. Int J Mol Sci. 15:8526–8538. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Dai BH, Geng L, Wang Y, Sui CJ, Xie F, Shen RX, Shen WF and Yang JM: microRNA-199a-5p protects hepatocytes from bile acid-induced sustained endoplasmic reticulum stress. Cell Death Dis. 4:e6042013. View Article : Google Scholar : PubMed/NCBI

18 

Shen Q, Cicinnati VR, Zhang X, Iacob S, Weber F, Sotiropoulos GC, Radtke A, Lu M, Paul A, Gerken G, et al: Role of microRNA-199a-5p and discoidin domain receptor 1 in human hepatocellular carcinoma invasion. Mol Cancer. 9:2272010. View Article : Google Scholar : PubMed/NCBI

19 

Hsu CY, Hsieh TH, Tsai CF, Tsai HP, Chen HS, Chang Y, Chuang HY, Lee JN, Hsu YL and Tsai EM: miRNA-199a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis. J Pathol. 232:330–343. 2014. View Article : Google Scholar

20 

Gui R, Li D, Qi G, Suhad A and Nie X: Inhibition of Grb2-mediated activation of MAPK signal transduction suppresses NOR1/CB1954-induced cytotoxicity in the HepG2 cell line. Oncol Lett. 4:566–570. 2012.

21 

Cai J, Yang C, Yang Q, Ding H, Jia J, Guo J, Wang J and Wang Z: Deregulation of let-7e in epithelial ovarian cancer promotes the development of resistance to cisplatin. Oncogenesis. 2:e752013. View Article : Google Scholar : PubMed/NCBI

22 

Goel HL and Mercurio AM: VEGF targets the tumour cell. Nat Rev Cancer. 13:871–882. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Nie X, Zhang B, Li X, Xiang J, Xiao B, Ma J, Zhou M, Zhu S, Lu H, Gui R, et al: Cloning, expression, and mutation analysis of NOR1, a novel human gene down-regulated in HNE1 naso-pharyngeal carcinoma cell line. J Cancer Res Clin Oncol. 129:410–414. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Lino Cardenas CL, Henaoui IS, Courcot E, Roderburg C, Cauffiez C, Aubert S, Copin MC, Wallaert B, Glowacki F, Dewaeles E, et al: miR-199a-5p Is upregulated during fibrogenic response to tissue injury and mediates TGFbeta-induced lung fibroblast activation by targeting caveolin-1. PLoS Genet. 9:e10032912013. View Article : Google Scholar : PubMed/NCBI

25 

Ogawa T, Enomoto M, Fujii H, Sekiya Y, Yoshizato K, Ikeda K and Kawada N: MicroRNA-221/222 upregulation indicates the activation of stellate cells and the progression of liver fibrosis. Gut. 61:1600–1609. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Sayed D and Abdellatif M: AKT-ing via microRNA. Cell Cycle. 9:3213–3217. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Magrelli A, Azzalin G, Salvatore M, Viganotti M, Tosto F, Colombo T, Devito R, Di Masi A, Antoccia A, Lorenzetti S, et al: Altered microRNA expression patterns in hepatoblastoma patients. Transl Oncol. 2:157–163. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Pencheva N, Tran H, Buss C, Huh D, Drobnjak M, Busam K and Tavazoie SF: Convergent multi-miRNA targeting of ApoE drives LRP1/LRP8-dependent melanoma metastasis and angiogenesis. Cell. 151:1068–1082. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Cheung HH, Davis AJ, Lee TL, Pang AL, Nagrani S, Rennert OM and Chan WY: Methylation of an intronic region regulates miR-199a in testicular tumor malignancy. Oncogene. 30:3404–3415. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Wang F, Zheng Z, Guo J and Ding X: Correlation and quantitation of microRNA aberrant expression in tissues and sera from patients with breast tumor. Gynecol Oncol. 119:586–593. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Augustin R, Endres K, Reinhardt S, Kuhn PH, Lichtenthaler SF, Hansen J, Wurst W and Trümbach D: Computational identification and experimental validation of microRNAs binding to the Alzheimer-related gene ADAM10. BMC Med Genet. 13:352012. View Article : Google Scholar : PubMed/NCBI

32 

Scherr M, Venturini L and Eder M: Lentiviral vector-mediated expression of pre-miRNAs and antagomiRs. Methods Mol Biol. 614:175–185. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Zeng Z, Zhou Y, Xiong W, Luo X, Zhang W, Li X, Fan S, Cao L, Tang K, Wu M, et al: Analysis of gene expression identifies candidate molecular markers in nasopharyngeal carcinoma using microdissection and cDNA microarray. J Cancer Res Clin Oncol. 133:71–81. 2007. View Article : Google Scholar

34 

Li W, Li X, Wang W, Li X, Tan Y, Yi M, Yang J, McCarthy JB, Xiong W, Wu M, et al: NOR1 is an HSF1- and NRF1-regulated putative tumor suppressor inactivated by promoter hyper-methylation in nasopharyngeal carcinoma. Carcinogenesis. 32:1305–1314. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gui R, Huang R, Zhang J, Wen X and Nie X: MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells. Oncol Rep 35: 2216-2222, 2016.
APA
Gui, R., Huang, R., Zhang, J., Wen, X., & Nie, X. (2016). MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells. Oncology Reports, 35, 2216-2222. https://doi.org/10.3892/or.2016.4550
MLA
Gui, R., Huang, R., Zhang, J., Wen, X., Nie, X."MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells". Oncology Reports 35.4 (2016): 2216-2222.
Chicago
Gui, R., Huang, R., Zhang, J., Wen, X., Nie, X."MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells". Oncology Reports 35, no. 4 (2016): 2216-2222. https://doi.org/10.3892/or.2016.4550
Copy and paste a formatted citation
x
Spandidos Publications style
Gui R, Huang R, Zhang J, Wen X and Nie X: MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells. Oncol Rep 35: 2216-2222, 2016.
APA
Gui, R., Huang, R., Zhang, J., Wen, X., & Nie, X. (2016). MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells. Oncology Reports, 35, 2216-2222. https://doi.org/10.3892/or.2016.4550
MLA
Gui, R., Huang, R., Zhang, J., Wen, X., Nie, X."MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells". Oncology Reports 35.4 (2016): 2216-2222.
Chicago
Gui, R., Huang, R., Zhang, J., Wen, X., Nie, X."MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells". Oncology Reports 35, no. 4 (2016): 2216-2222. https://doi.org/10.3892/or.2016.4550
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