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Article

SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury

  • Authors:
    • Kaichun Li
    • Mingzhen Ying
    • Dan Feng
    • Yan Chen
    • Jingwen Wang
    • Yajie Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Changhai Hospital Affiliated to The Second Military Medical University, Shanghai 200433, P.R. China, Department of Pathology, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China, Department of Oncology, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China
  • Pages: 2405-2412
    |
    Published online on: January 15, 2016
       https://doi.org/10.3892/or.2016.4564
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Abstract

Triple-negative breast cancer (TNBC) is a special subtype of breast cancer, which is characterized by the negative form of estrogen receptor (ER), progesterone receptor (PR) and human epithelial growth factor receptor 2 (HER2). TNBC accounts for ~15% of all breast cancer forms, and often leads to high mortality and poor prognosis. Structural maintenance of chromosome 1 (SMC1) is a subunit of the cohesion protein complex. Brachyury is a protein that is encoded by the T gene in humans, which is a transcription factor within the T-box complex of genes. Epithelial-mesenchymal transition (EMT) is a ubiquitous process in the body, and in particular, induces metastasis and the proliferation of cancer cells. In the present study, we found that SMC1 expression in TNBC tissues exceeded its expression in adjacent non-tumor tissues. Similarly, the expression of SMC1 in TNBC cell lines (hs578T and HCC1937) was found to be higher than in MCF10a and MCF7 cells. Subsequently, SMC1 was overexpressed and silenced in hs578T and HCC1937 cells through plasmid and siRNA transfection, respectively. The results showed that the high expression of SMC1 often promoted EMT, accompanied by the enhanced expression of Brachyury. Besides, upregulated expression of Brachyury through plasmid transfection also significantly improved the level of EMT, which further indicated that SMC1 increased EMT in TNBC through the induction of Brachyury expression. Taken together, these results contributed to a better understanding of the pathogenesis of TNBC, which also provided an experimental basis for the prevention, diagnosis and treatment of TNBC.
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Copy and paste a formatted citation
Spandidos Publications style
Li K, Ying M, Feng D, Chen Y, Wang J and Wang Y: SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury. Oncol Rep 35: 2405-2412, 2016.
APA
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., & Wang, Y. (2016). SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury. Oncology Reports, 35, 2405-2412. https://doi.org/10.3892/or.2016.4564
MLA
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., Wang, Y."SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury". Oncology Reports 35.4 (2016): 2405-2412.
Chicago
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., Wang, Y."SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury". Oncology Reports 35, no. 4 (2016): 2405-2412. https://doi.org/10.3892/or.2016.4564
Copy and paste a formatted citation
x
Spandidos Publications style
Li K, Ying M, Feng D, Chen Y, Wang J and Wang Y: SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury. Oncol Rep 35: 2405-2412, 2016.
APA
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., & Wang, Y. (2016). SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury. Oncology Reports, 35, 2405-2412. https://doi.org/10.3892/or.2016.4564
MLA
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., Wang, Y."SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury". Oncology Reports 35.4 (2016): 2405-2412.
Chicago
Li, K., Ying, M., Feng, D., Chen, Y., Wang, J., Wang, Y."SMC1 promotes epithelial-mesenchymal transition in triple-negative breast cancer through upregulating Brachyury". Oncology Reports 35, no. 4 (2016): 2405-2412. https://doi.org/10.3892/or.2016.4564
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