Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer

  • Authors:
    • Yifan Li
    • Duqun Chen
    • Yuchi Li
    • Lu Jin
    • Jiaju Liu
    • Zhengming Su
    • Zhengyu Qi
    • Min Shi
    • Zhimao Jiang
    • Liangchao Ni
    • Shangqi Yang
    • Yaoting Gui
    • Xiangming Mao
    • Yun Chen
    • Yongqing Lai
  • View Affiliations

  • Published online on: January 20, 2016     https://doi.org/10.3892/or.2016.4579
  • Pages: 1967-1978
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Abstract

Renal cell carcinoma (RCC) is the most common kidney cancer in adults and has a poor prognosis. cAMP responsive element binding protein 1 (CREB1) is a proto‑oncogenic transcription factor involved in malignancies of various organs. However, its functional role(s) have not yet been elucidated in RCC. We investigated the expression pattern, function and regulation of CREB1 in RCC. CREB1 was overexpressed in the RCC tissues and cell lines. Downregulation of CREB1 inhibited RCC tumorigenesis by affecting cell proliferation, migration and apoptosis. Multiple computational algorithms predicted that the 3'‑untranslated region (3'‑UTR) of human CREB1 mRNA is a target for miR‑10b‑5p and miR‑363‑3p. Luciferase reporter assay, qPCR and western blot analysis confirmed that miR‑10b‑5p and miR‑363‑3p bind directly to the 3'‑UTR of CREB1 mRNA and inhibit mRNA and protein expression of CREB1. qPCR data also revealed a significantly lower expression of miR‑10b‑5p and miR‑363‑3p in RCC tissues. Introduction of miR‑10b‑5p and miR‑363‑3p mimics led to suppressed expression of CREB1 and inhibited cell proliferation, migration and apoptosis reduction. Taken together, we propose that CREB1 is an oncogene in RCC and that upregulation of CREB1 by loss of tumor suppressive miR‑10b‑5p and miR‑363‑3p plays an important role in the tumorigenesis of RCC.
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April-2016
Volume 35 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li Y, Chen D, Li Y, Jin L, Liu J, Su Z, Qi Z, Shi M, Jiang Z, Ni L, Ni L, et al: Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer. Oncol Rep 35: 1967-1978, 2016
APA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z. ... Lai, Y. (2016). Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer. Oncology Reports, 35, 1967-1978. https://doi.org/10.3892/or.2016.4579
MLA
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Ni, L., Yang, S., Gui, Y., Mao, X., Chen, Y., Lai, Y."Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer". Oncology Reports 35.4 (2016): 1967-1978.
Chicago
Li, Y., Chen, D., Li, Y., Jin, L., Liu, J., Su, Z., Qi, Z., Shi, M., Jiang, Z., Ni, L., Yang, S., Gui, Y., Mao, X., Chen, Y., Lai, Y."Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer". Oncology Reports 35, no. 4 (2016): 1967-1978. https://doi.org/10.3892/or.2016.4579