miR-137 acts as a tumor suppressor in papillary thyroid carcinoma by targeting CXCL12 Retraction in /10.3892/or.2021.8157

Dong,Su; Jin,Meishan; Li,Ye; Ren,Peiyou; Liu,Jia

doi:10.3892/or.2016.4604

miR-137 acts as a tumor suppressor in papillary thyroid carcinoma by targeting CXCL12

Retraction in: /10.3892/or.2021.8157

Authors:
- Su Dong
- Meishan Jin
- Ye Li
- Peiyou Ren
- Jia Liu
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Affiliations: Department of Anesthesia, The First Hospital of Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China, Department of Pathology, The First Hospital of Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China, Department of Radiology, The First Hospital of Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China, Department of Thyroid Surgery, The First Hospital of Jilin University, Chaoyang, Changchun, Jilin 130021, P.R. China
Published online on: February 1, 2016 https://doi.org/10.3892/or.2016.4604
Pages: 2151-2158

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Abstract

Accumulating evidence has shown that aberrantly expressed microRNAs (miRs) are extensively involved in tumorigenesis. microRNA-137 (miR-137) has been reported as a tumor suppressor in various types of cancer. However, the biological function and underlying molecular mechanism of miR-137 in papillary thyroid carcinoma (PTC) remain largely unknown. Therefore, the present study aimed to investigate the expression pattern of miR-137 and its functional significance in PTC. Quantitative RT-PCR (qRT-PCR) assay showed that miR-137 expression was significantly downregulated in human PTC tissues, and its expression was significantly negatively correlated with tumor-node-metastasis (TNM) stage and lymph node metastasis. Functional assays showed that forced expression of miR-137 in PTC cells significantly inhibited proliferation, colony formation, migration and invasion in vitro. Importantly, on the basis of bioinformatic analysis and luciferase reporter assay, we found that miR-137 directly targeted the 3'-untranslated region (3'-UTR) of C-X-C motif chemokine 12 (also known as SDF-1) (CXCL12). qRT-PCR and western blot analysis further verified the results and demonstrated that miR-137 could downregulate CXCL12 expression in PTC cells. We also confirmed that CXCL12 expression was increased in PTC tissues and was inversely correlated with miR-137. In addition, our results also showed that downregulation of CXCL12 mimicked the effects of miR-137 overexpression, and upregulation of CXCL12 partially reversed the inhibitory effects of miR-137 in PTC cells. These results showed that miR-137 may function as a tumor suppressor in PTC by targeting CXCL12, suggesting that miR-137 may act as a potential target for PTC treatment.

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