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Article

SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway

  • Authors:
    • Mingshan Hou
    • Xiaobing Liu
    • Jie Cao
    • Bo Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Shaanxi Provincial People's Hospital, The Affiliated Hospital of Xi'an Medical University, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710068, P.R. China
  • Pages: 2003-2010
    |
    Published online on: February 3, 2016
       https://doi.org/10.3892/or.2016.4609
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Abstract

Glioma cell metastasis is a serious obstacle for surgical treatment and prognosis, of which locomotion of the cytoskeleton is a key contributor of cancer cell spreading. SEPT7 is documented as a cytoskeletal protein with GTPase activity and involved in glioma progression. However, the underlying mechanism of SEPT7 in glioma invasion remains unresolved. Our study investigated whether SEPT7 influences glioma cell migration involved in cytoskeleton modulation. The SEPT7 expression in various glioma cell lines was markedly decreased compared to in normal human brain cells. It was demonstrated that SEPT7 overexpression significantly inhibits LN18 cell migration and chemotaxis induced by IGF‑1 (P<0.01 and P<0.01). Moreover, MMP‑2 and MMP‑9 were dramatically depressed after SEPT7 upregulation. To understand the mechanisms by which SEPT7 modulates homeostasis of the actin cytoskeleton, the F‑actin/G‑actin ratio and cofilin expression were determined. The data revealed that the F‑actin/G‑actin ratio and cofilin were reduced, and p‑cofilin increased conversely in cells with SEPT7 overexpression, indicating that SEPT7 reduced glioma cell migration by promoting cofilin phosphorylation and depolymerizing actin. Then, to understand the role of cofilin in SEPT7‑mediated actin dynamic equilibrium and cell migration, cofilin siRNA was transfected into cells. Surprisingly, cell migration and actin polymerization which had been improved by SEPT7 siRNA were significantly reversed, and the accompanying cofilin phosphorylation increased, indicating that cofilin phospho‑regulation played an important role in SEPT7‑mediated cytoskeleton locomotion and glioma cell migration. In conclusion, SEPT7 is involved in glioma cell migration with the assistance of cofilin phospho‑mediated cytoskeleton locomotion.
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1 

Ohgaki H and Kleihues P: Epidemiology and etiology of gliomas. Acta Neuropathol. 109:93–108. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Claes A, Idema AJ and Wesseling P: Diffuse Glioma growth: A guerilla war. Acta Neuropathol. 114:443–458. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Giese A and Westphal M: Glioma invasion in the central nervous system. Neurosurgery. 39:235–250; discussion 250–252. 1996. View Article : Google Scholar : PubMed/NCBI

4 

Meyer MA: Malignant gliomas in adults. N Engl J Med. 359:1850author reply 1850. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Mittal S, Szlaczky MC and Barger GR: Low-grade gliomas in adults. Curr Treat Options Neurol. 10:271–284. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Wen PY and Kesari S: Malignant Gliomas in adults. N Engl J Med. 359:492–507. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Laws ER, Shaffrey ME, Morris A and Anderson FA Jr: Surgical management of intracranial gliomas - does radical resection improve outcome? Acta Neurochir (Suppl). 85:47–53. 2003. View Article : Google Scholar

8 

Simon M and Schramm J: Surgical management of intracranial gliomas. Recent Results Cancer Res. 171:105–124. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Jia ZF, Huang Q, Kang CS, Yang WD, Wang GX, Yu SZ, Jiang H and Pu PY: Overexpression of septin 7 suppresses glioma cell growth. J Neurooncol. 98:329–340. 2010. View Article : Google Scholar

10 

Hall PA, Jung K, Hillan KJ and Russell SE: Expression profiling the human septin gene family. J Pathol. 206:269–278. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Jiang H, Hua D, Zhang J, Lan Q, Huang Q, Yoon JG, Han X, Li L, Foltz G, Zheng S, et al: MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7. Oncol Rep. 31:2261–2269. 2014.PubMed/NCBI

12 

Xu S, Jia ZF, Kang C, Huang Q, Wang G, Liu X, Zhou X, Xu P and Pu P: Upregulation of SEPT7 gene inhibits invasion of human glioma cells. Cancer Invest. 28:248–258. 2010. View Article : Google Scholar

13 

Bravo-Cordero JJ, Magalhaes MA, Eddy RJ, Hodgson L and Condeelis J: Functions of cofilin in cell locomotion and invasion. Nat Rev Mol Cell Biol. 14:405–415. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Mizuno K: Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation. Cell Signal. 25:457–469. 2013. View Article : Google Scholar

15 

Gladfelter AS: Cytoskeleton: Cirque du septins. Curr Biol. 24:R526–R528. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Brooks PC, Strömblad S, Sanders LC, von Schalscha TL, Aimes RT, Stetler-Stevenson WG, Quigley JP and Cheresh DA: Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin alpha v beta 3. Cell. 85:683–693. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Ramos-DeSimone N, Hahn-Dantona E, Sipley J, Nagase H, French DL and Quigley JP: Activation of matrix metallopro-teinase-9 (MMP-9) via a converging plasmin/stromelysin-1 cascade enhances tumor cell invasion. J Biol Chem. 274:13066–13076. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Hu J, Bai X, Bowen JR, Dolat L, Korobova F, Yu W, Baas PW, Svitkina T, Gallo G and Spiliotis ET: Septin-driven coordination of actin and microtubule remodeling regulates the collateral branching of axons. Curr Biol. 22:1109–1115. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Mavrakis M, Azou-Gros Y, Tsai FC, Alvarado J, Bertin A, Iv F, Kress A, Brasselet S, Koenderink GH and Lecuit T: Septins promote F-actin ring formation by crosslinking actin filaments into curved bundles. Nat Cell Biol. 16:322–334. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Agudelo-garcia PA, De Jesus JK, Williams SP, Nowicki MO, Chiocca EA, Liyanarachchi S, Li PK, Lannutti JJ, Johnson JK, Lawler SE, et al: Glioma cell migration on three-dimensional nanofiber scaffolds is regulated by substrate topography and abolished by inhibition of STAT3 signaling. Neoplasia. 13:831–840. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Rao JS: Molecular mechanisms of glioma invasiveness: The role of proteases. Nat Rev Cancer. 3:489–501. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Yamaguchi H and Condeelis J: Regulation of the actin cyto-skeleton in cancer cell migration and invasion. Biochim Biophys Acta. 1773:642–652. 2007. View Article : Google Scholar

23 

Sung BH, Zhu X, Kaverina I and Weaver AM: Cortactin controls cell motility and lamellipodial dynamics by regulating ECM secretion. Curr Biol. 21:1460–1469. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Yumura S, Itoh G, Kikuta Y, Kikuchi T, Kitanishi-Yumura T and Tsujioka M: Cell-scale dynamic recycling and cortical flow of the actin-myosin cytoskeleton for rapid cell migration. Biol Open. 2:200–209. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Murrell MP and Gardel ML: F-actin buckling coordinates contractility and severing in a biomimetic actomyosin cortex. Proc Natl Acad Sci USA. 109:20820–20825. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Vogel SK, Petrasek Z, Heinemann F and Schwille P: Myosin motors fragment and compact membrane-bound actin filaments. eLife. 2:e001162013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hou M, Liu X, Cao J and Chen B: SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway. Oncol Rep 35: 2003-2010, 2016.
APA
Hou, M., Liu, X., Cao, J., & Chen, B. (2016). SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway. Oncology Reports, 35, 2003-2010. https://doi.org/10.3892/or.2016.4609
MLA
Hou, M., Liu, X., Cao, J., Chen, B."SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway". Oncology Reports 35.4 (2016): 2003-2010.
Chicago
Hou, M., Liu, X., Cao, J., Chen, B."SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway". Oncology Reports 35, no. 4 (2016): 2003-2010. https://doi.org/10.3892/or.2016.4609
Copy and paste a formatted citation
x
Spandidos Publications style
Hou M, Liu X, Cao J and Chen B: SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway. Oncol Rep 35: 2003-2010, 2016.
APA
Hou, M., Liu, X., Cao, J., & Chen, B. (2016). SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway. Oncology Reports, 35, 2003-2010. https://doi.org/10.3892/or.2016.4609
MLA
Hou, M., Liu, X., Cao, J., Chen, B."SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway". Oncology Reports 35.4 (2016): 2003-2010.
Chicago
Hou, M., Liu, X., Cao, J., Chen, B."SEPT7 overexpression inhibits glioma cell migration by targeting the actin cytoskeleton pathway". Oncology Reports 35, no. 4 (2016): 2003-2010. https://doi.org/10.3892/or.2016.4609
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