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Article

MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma

  • Authors:
    • Jiangqiao Geng
    • Yuanhu Liu
    • Yaqiong Jin
    • Jun Tai
    • Jie Zhang
    • Xiao Xiao
    • Ping Chu
    • Yongbo Yu
    • Sheng Cai Wang
    • Jie Lu
    • Shujing Han
    • Jin Shi
    • Yongli Guo
    • Xin Ni
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China, Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China
  • Pages: 2017-2026
    |
    Published online on: February 11, 2016
       https://doi.org/10.3892/or.2016.4617
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Abstract

MicroRNAs (miRNAs) are increasingly recognized as oncogenes or tumor suppressors in laryngeal squamous cell carcinoma (LSCC). In this study, we analyzed the roles of miR-365a-3p, miR-143-5p, and miR-494-3p in LSCC using Annexin V/propidium iodide double staining and flow cyto-metry, along with a Transwell migration and invasion assay. The results showed that miR-365a-3p inhibitor significantly facilitated cell apoptosis and suppressed cell cycle progression, migration, and invasion in Hep-2 cells. However, miR-143-5p and miR-494-3p had no such influences. We then investigated the role of miR-365a-3p in LSCC in vivo and found that miR-365a-3p inhibitor suppressed LSCC xenograft tumor growth and metastasis in xenograft mouse models. Moreover, miR-365a-3p inhibitor significantly decreased the expression of p-AKT (Ser473), which indicated that miR-365a-3p can mediate PI3K/AKT signaling pathway transduction via p-AKT (Ser473) in LSCC. The data suggest that miR-365a-3p may act as an oncomiR and may promote growth and metastasis in LSCC via the PI3K/AKT signaling pathway, and thus miR‑365a-3p may be a potential therapeutic target for treatment of LSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Geng J, Liu Y, Jin Y, Tai J, Zhang J, Xiao X, Chu P, Yu Y, Wang SC, Lu J, Lu J, et al: MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma. Oncol Rep 35: 2017-2026, 2016.
APA
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X. ... Ni, X. (2016). MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma. Oncology Reports, 35, 2017-2026. https://doi.org/10.3892/or.2016.4617
MLA
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X., Chu, P., Yu, Y., Wang, S. C., Lu, J., Han, S., Shi, J., Guo, Y., Ni, X."MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma". Oncology Reports 35.4 (2016): 2017-2026.
Chicago
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X., Chu, P., Yu, Y., Wang, S. C., Lu, J., Han, S., Shi, J., Guo, Y., Ni, X."MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma". Oncology Reports 35, no. 4 (2016): 2017-2026. https://doi.org/10.3892/or.2016.4617
Copy and paste a formatted citation
x
Spandidos Publications style
Geng J, Liu Y, Jin Y, Tai J, Zhang J, Xiao X, Chu P, Yu Y, Wang SC, Lu J, Lu J, et al: MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma. Oncol Rep 35: 2017-2026, 2016.
APA
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X. ... Ni, X. (2016). MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma. Oncology Reports, 35, 2017-2026. https://doi.org/10.3892/or.2016.4617
MLA
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X., Chu, P., Yu, Y., Wang, S. C., Lu, J., Han, S., Shi, J., Guo, Y., Ni, X."MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma". Oncology Reports 35.4 (2016): 2017-2026.
Chicago
Geng, J., Liu, Y., Jin, Y., Tai, J., Zhang, J., Xiao, X., Chu, P., Yu, Y., Wang, S. C., Lu, J., Han, S., Shi, J., Guo, Y., Ni, X."MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell carcinoma". Oncology Reports 35, no. 4 (2016): 2017-2026. https://doi.org/10.3892/or.2016.4617
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