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Article

Radiosensitization effect of Huaier on breast cancer cells

  • Authors:
    • Xia Ding
    • Qifeng Yang
    • Xiangnan Kong
    • Bruce G. Haffty
    • Sumei Gao
    • Meena S. Moran
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Laboratory Medicine Center, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong 266035, P.R. China, Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey and Rutgers - Robert Wood Johnson Medical School, New Brunswick, NJ, USA, Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, USA
  • Pages: 2843-2850
    |
    Published online on: February 23, 2016
       https://doi.org/10.3892/or.2016.4630
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Abstract

Radiotherapy is a critical treatment strategy for breast cancer. However, its wide application is sometimes restricted by radioresistance and radiotoxicity. Trametes robiniophila Murr. (Huaier), an officinal fungus used as a traditional Chinese medicine (TCM), is reported to have multi-biological functions during cancer treatment. Yet, its radiosensitization effects have not been evaluated to date. In the present study, using HTA 2.0 transcriptome microarray assay, Huaier was found to downregulate genes related to the cell cycle, cell division, cell cycle phases and DNA repair. This investigation utilized a colony formation assay to confirm the ability of Huaier to sensitize breast cancer cells to radiotherapy. Flow cytometry, immunofluorescence staining and western blotting were used to illustrate the sensitization mechanism. Our findings suggest that Huaier causes G0/G1 arrest through downregulation of cell cycle-regulating proteins in MCF-7 and MDA-MB-468 cells, prolongs the persistence of γ-H2Ax foci after radiotherapy and interferes with the homologous recombination (HR) pathway of DNA repair by downregulating RAD51. These results suggest that Huaier has the ability to sensitize breast cancer cells to radiotherapy through regulation of the cell cycle and DNA repair pathway. Thus, Huaier may be a promising radiosensitizer for the treatment of breast cancer.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Brown LC, Mutter RW and Halyard MY: Benefits, risks, and safety of external beam radiation therapy for breast cancer. Int J Womens Health. 7:449–458. 2015.PubMed/NCBI

3 

Gerweck LE, Vijayappa S, Kurimasa A, Ogawa K and Chen DJ: Tumor cell radiosensitivity is a major determinant of tumor response to radiation. Cancer Res. 66:8352–8355. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Uma Devi P: Normal tissue protection in cancer therapy -progress and prospects. Acta Oncol. 37:247–252. 1998. View Article : Google Scholar

5 

Thomson D, Yang H, Baines H, Miles E, Bolton S, West C and Slevin N: NIMRAD - a phase III trial to investigate the use of nimorazole hypoxia modification with intensity-modulated radiotherapy in head and neck cancer. Clin Oncol. 26:344–347. 2014. View Article : Google Scholar

6 

Ang KK: More lessons learned from the suffocation of hypoxia. J Clin Oncol. 28:2941–2943. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Cragg GM and Newman DJ: Plants as a source of anti-cancer agents. J Ethnopharmacol. 100:72–79. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Chen P, Li J, Jiang HG, Lan T and Chen YC: Curcumin reverses cisplatin resistance in cisplatin-resistant lung cancer cells by inhibiting FA/BRCA pathway. Tumour Biol. 36:3591–3599. 2015. View Article : Google Scholar

9 

Shi XP, Miao S, Wu Y, Zhang W, Zhang XF, Ma HZ, Xin HL, Feng J, Wen AD and Li Y: Resveratrol sensitizes tamoxifen in antiestrogen-resistant breast cancer cells with epithelial-mesenchymal transition features. Int J Mol Sci. 14:15655–15668. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Srivastava V, Negi AS, Kumar JK, Gupta MM and Khanuja SP: Plant-based anticancer molecules: A chemical and biological profile of some important leads. Bioorg Med Chem. 13:5892–5908. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Li LYS, Wang Y and Tang Z: Progress on experimental research and clinical application of Trametes robiniophila. China Cancer. 16:110–113. 2006.

12 

Zheng J, Li C, Wu X, Liu M, Sun X, Yang Y, Hao M, Sheng S, Sun Y, Zhang H, et al: Huaier polysaccharides suppress hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway. Int J Biol Macromol. 64:106–110. 2014. View Article : Google Scholar

13 

Li C, Wu X, Zhang H, Yang G, Hao M, Sheng S, Sun Y, Long J, Hu C, Sun X, et al: A Huaier polysaccharide reduced metastasis of human hepatocellular carcinoma SMMC-7721 cells via modu-lating AUF-1 signaling pathway. Tumour Biol. 36:6285–6293. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Zhang T, Wang K, Zhang J, Wang X, Chen Z, Ni C, Qiu F and Huang J: Huaier aqueous extract inhibits colorectal cancer stem cell growth partially via downregulation of the Wnt/β-catenin pathway. Oncol Lett. 5:1171–1176. 2013.PubMed/NCBI

15 

Wang X, Zhang N, Huo Q and Yang Q: Anti-angiogenic and antitumor activities of Huaier aqueous extract. Oncol Rep. 28:1167–1175. 2012.PubMed/NCBI

16 

Zhang N, Kong X, Yan S, Yuan C and Yang Q: Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis. Cancer Sci. 101:2375–2383. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Wu T, Chen W, Liu S, Lu H, Wang H, Kong D, Huang X, Kong Q, Ning Y and Lu Z: Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p. FEBS Lett. 588:2107–2114. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Zhang F, Zhang Z and Liu Z: Effects of Huaier aqueous extract on proliferation and apoptosis in the melanoma cell line A875. Acta Histochem. 115:705–711. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Cui Y, Meng H, Liu W, Wang H and Liu Q: Huaier aqueous extract induces apoptosis of human fibrosarcoma HT1080 cells through the mitochondrial pathway. Oncol Lett. 9:1590–1596. 2015.PubMed/NCBI

20 

Li C, Wu X, Zhang H, Yang G, Hao M, Sheng S, Sun Y, Long J, Hu C, Sun X, et al: A Huaier polysaccharide inhibits hepatocellular carcinoma growth and metastasis. Tumour Biol. 36:1739–1745. 2015. View Article : Google Scholar

21 

Sun Y, Sun T, Wang F, Zhang J, Li C, Chen X, Li Q and Sun S: A polysaccharide from the fungi of Huaier exhibits anti-tumor potential and immunomodulatory effects. Carbohydr Polym. 92:577–582. 2013. View Article : Google Scholar

22 

Yu Zhe WT and Yang Z: The reversal effects of Trametes Robiniophila Murr. on multidrug resistance in resistant human hepatocellular carcinoma cell line BEL-7402/5-Fu. J Clin Oncol. 19:443–447. 2013.

23 

Kong X, Ding X and Yang Q: Identification of multi-target effects of Huaier aqueous extract via microarray profiling in triple-negative breast cancer cells. Int J Oncol. 46:2047–2056. 2015.PubMed/NCBI

24 

Gene Ontology Consortium: The Gene Ontology (GO) project in 2006. Nucleic Acids Res. 34:D322–D326. 2006. View Article : Google Scholar :

25 

Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al The Gene Ontology Consortium: Gene ontology: Tool for the unification of biology. Nat Genet. 25:25–29. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Franken NA, Rodermond HM, Stap J, Haveman J and van Bree C: Clonogenic assay of cells in vitro. Nat Protoc. 1:2315–2319. 2006. View Article : Google Scholar

27 

Gerić M, Gajski G and Garaj-Vrhovac V: γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology. Ecotoxicol Environ Saf. 105:13–21. 2014. View Article : Google Scholar

28 

Cohen I, Tagliaferri M and Tripathy D: Traditional Chinese medicine in the treatment of breast cancer. Semin Oncol. 29:563–574. 2002. View Article : Google Scholar

29 

Wong KY, Tan EY, Chen JJ, Teo C and Chan PM: The use of traditional Chinese medicine among breast cancer patients: Implications for the clinician. Ann Acad Med Singapore. 43:74–78. 2014.PubMed/NCBI

30 

Wang X, Zhang N, Huo Q, Sun M, Lv S and Yang Q: Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor α signaling. Int J Oncol. 43:321–328. 2013.PubMed/NCBI

31 

Busse PM, Bose SK, Jones RW and Tolmach LJ: The action of caffeine on X-irradiated HeLa cells. III. Enhancement of X-ray-induced killing during G2 arrest. Radiat Res. 76:292–307. 1978. View Article : Google Scholar : PubMed/NCBI

32 

Wang J, Liu Q and Yang Q: Radiosensitization effects of berberine on human breast cancer cells. Int J Mol Med. 30:1166–1172. 2012.PubMed/NCBI

33 

Giacinti C and Giordano A: RB and cell cycle progression. Oncogene. 25:5220–5227. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Núñez MI, McMillan TJ, Valenzuela MT, Ruiz de Almodóvar JM and Pedraza V: Relationship between DNA damage, rejoining and cell killing by radiation in mammalian cells. Radiother Oncol. 39:155–165. 1996. View Article : Google Scholar : PubMed/NCBI

35 

Kuo LJ and Yang LX: Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In Vivo. 22:305–309. 2008.PubMed/NCBI

36 

Shibata D: When does MMR loss occur during HNPCC progression? Cancer Biomark. 2:29–35. 2006.PubMed/NCBI

37 

Powell SN and Kachnic LA: Therapeutic exploitation of tumor cell defects in homologous recombination. Anticancer Agents Med Chem. 8:448–460. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ding X, Yang Q, Kong X, Haffty BG, Gao S and Moran MS: Radiosensitization effect of Huaier on breast cancer cells. Oncol Rep 35: 2843-2850, 2016.
APA
Ding, X., Yang, Q., Kong, X., Haffty, B.G., Gao, S., & Moran, M.S. (2016). Radiosensitization effect of Huaier on breast cancer cells. Oncology Reports, 35, 2843-2850. https://doi.org/10.3892/or.2016.4630
MLA
Ding, X., Yang, Q., Kong, X., Haffty, B. G., Gao, S., Moran, M. S."Radiosensitization effect of Huaier on breast cancer cells". Oncology Reports 35.5 (2016): 2843-2850.
Chicago
Ding, X., Yang, Q., Kong, X., Haffty, B. G., Gao, S., Moran, M. S."Radiosensitization effect of Huaier on breast cancer cells". Oncology Reports 35, no. 5 (2016): 2843-2850. https://doi.org/10.3892/or.2016.4630
Copy and paste a formatted citation
x
Spandidos Publications style
Ding X, Yang Q, Kong X, Haffty BG, Gao S and Moran MS: Radiosensitization effect of Huaier on breast cancer cells. Oncol Rep 35: 2843-2850, 2016.
APA
Ding, X., Yang, Q., Kong, X., Haffty, B.G., Gao, S., & Moran, M.S. (2016). Radiosensitization effect of Huaier on breast cancer cells. Oncology Reports, 35, 2843-2850. https://doi.org/10.3892/or.2016.4630
MLA
Ding, X., Yang, Q., Kong, X., Haffty, B. G., Gao, S., Moran, M. S."Radiosensitization effect of Huaier on breast cancer cells". Oncology Reports 35.5 (2016): 2843-2850.
Chicago
Ding, X., Yang, Q., Kong, X., Haffty, B. G., Gao, S., Moran, M. S."Radiosensitization effect of Huaier on breast cancer cells". Oncology Reports 35, no. 5 (2016): 2843-2850. https://doi.org/10.3892/or.2016.4630
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