Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis

  • Authors:
    • Xiaofeng Tang
    • Jianjun Tang
    • Xia Liu
    • Lei Zeng
    • Chun Cheng
    • Yanqin Luo
    • Liping Li
    • Shu-Lan Qin
    • Yi Sang
    • Liang-Ming Deng
    • Xiao-Bin Lv
  • View Affiliations

  • Published online on: March 2, 2016     https://doi.org/10.3892/or.2016.4647
  • Pages: 2963-2969
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Abstract

Aberrant expression of the miR-129 family has been found in several types of cancer, yet its expression and potential biologic role in breast cancer remain largely unknown. In the present study, we found that miR-129-2 was consistently downregulated in the breast cancer specimens and cell lines. Overexpression of miR-129-2-3p markedly suppressed breast cancer cell proliferation and induced its apoptosis. In addition, a luciferase reporter assay revealed that miR-129-2-3p suppressed BCL2L2 expression. Furthermore, BCL2L2 was able to reverse miR-129-2-3p-mediated cell apoptosis, indicating that BCL2L2 plays a crucial role in mediating the tumor-suppressive role of miR-129-2-3p. Moreover, bisulfite DNA sequencing PCR (BSP) analysis identified that promoter hypermethylation was responsible for the downregulation of miR-129-2 in breast cancer. Collectively, our findings indicate that miR-129-2 is downregulated in breast cancer cells by promoter hypermethylation. Moreover, downregulation of miR-129-2 results in BCL2L2 overexpression and disease progression in breast cancer patients.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tang X, Tang J, Liu X, Zeng L, Cheng C, Luo Y, Li L, Qin S, Sang Y, Deng L, Deng L, et al: Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis. Oncol Rep 35: 2963-2969, 2016
APA
Tang, X., Tang, J., Liu, X., Zeng, L., Cheng, C., Luo, Y. ... Lv, X. (2016). Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis. Oncology Reports, 35, 2963-2969. https://doi.org/10.3892/or.2016.4647
MLA
Tang, X., Tang, J., Liu, X., Zeng, L., Cheng, C., Luo, Y., Li, L., Qin, S., Sang, Y., Deng, L., Lv, X."Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis". Oncology Reports 35.5 (2016): 2963-2969.
Chicago
Tang, X., Tang, J., Liu, X., Zeng, L., Cheng, C., Luo, Y., Li, L., Qin, S., Sang, Y., Deng, L., Lv, X."Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis". Oncology Reports 35, no. 5 (2016): 2963-2969. https://doi.org/10.3892/or.2016.4647