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Article

Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells

  • Authors:
    • Ai-Ping Le
    • Lun-Li Zhang
    • Wei Liu
    • Yu-Fei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Infectious Diseases, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 2970-2976
    |
    Published online on: March 17, 2016
       https://doi.org/10.3892/or.2016.4684
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Abstract

The present study was designed to investigate the effect of cantharidin on cell proliferation, ability of selfrenewal, cell cycle arrest and induction of apoptosis in HepG2 hepatocellular carcinoma stem cells (HCSCs). It was observed that cantharidin treatment exhibited dose- and time-dependent inhibitory effect on the viability of HCSCs. The inhibition of cell viability by cantharidin in HepG2 CD133+ and parental cells was significant at the concentration 5 and 15 µM, respectively after 48 h. Cantharidin treatment inhibited the self-renewal ability of the HCSCs and the expression of β-catenin and cyclin D1. Flow cytometry revealed that cantharidin treatment at 5 µM concentration significantly increased the cell population in G2/M phase and decreased the population in the G1 phase. Cantharidin treatment in the HCSCs for 48 h increased expression of histone H2AX, Myt1, cyclin A2, cyclin B1, p53 and cdc2 (Tyr15) phosphorylation significantly compared to the parental cells. Exposure of the HCSCs to cantharidin for 48 h at a concentration of 5 µM caused a significant increase in the proportion of apoptotic cells. Therefore, cantharidin is a promising agent for the hepatocellular carcinoma treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Le A, Zhang L, Liu W and Shi Y: Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells. Oncol Rep 35: 2970-2976, 2016.
APA
Le, A., Zhang, L., Liu, W., & Shi, Y. (2016). Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells. Oncology Reports, 35, 2970-2976. https://doi.org/10.3892/or.2016.4684
MLA
Le, A., Zhang, L., Liu, W., Shi, Y."Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells". Oncology Reports 35.5 (2016): 2970-2976.
Chicago
Le, A., Zhang, L., Liu, W., Shi, Y."Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells". Oncology Reports 35, no. 5 (2016): 2970-2976. https://doi.org/10.3892/or.2016.4684
Copy and paste a formatted citation
x
Spandidos Publications style
Le A, Zhang L, Liu W and Shi Y: Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells. Oncol Rep 35: 2970-2976, 2016.
APA
Le, A., Zhang, L., Liu, W., & Shi, Y. (2016). Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells. Oncology Reports, 35, 2970-2976. https://doi.org/10.3892/or.2016.4684
MLA
Le, A., Zhang, L., Liu, W., Shi, Y."Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells". Oncology Reports 35.5 (2016): 2970-2976.
Chicago
Le, A., Zhang, L., Liu, W., Shi, Y."Cantharidin inhibits cell proliferation and induces apoptosis through G2/M phase cell cycle arrest in hepatocellular carcinoma stem cells". Oncology Reports 35, no. 5 (2016): 2970-2976. https://doi.org/10.3892/or.2016.4684
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