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Article

Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells

  • Authors:
    • Meng-Yuan Dai
    • Yan Wang
    • Chen Chen
    • Fen Li
    • Bo-Kui Xiao
    • Shi-Ming Chen
    • Ze-Zhang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Otolaryngology-Head and Neck Surgery Institute, Medical School of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 2657-2664
    |
    Published online on: March 17, 2016
       https://doi.org/10.3892/or.2016.4689
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Abstract

The dietary compound phenethyl isothiocyanate (PEITC), an important tumoricidal component found in cruciferous vegetables, exhibits strong anticancer and chemopreventive effects in a variety of tumors. However, its role in human laryngeal cancer is unclear. The aim of the present study was to investigate whether PEITC exhibits anticancer properties in human laryngeal carcinoma Hep-2 cells in vitro and to identify the potential molecular mechanisms. The results showed that treatment of Hep-2 cells with PEITC significantly inhibited cell proliferation in a dose- and time-dependent manner, promoted apoptosis with concurrent G2/M cell cycle arrest and inhibited cell invasion in a dose-dependent manner. These effects were accompanied by significant alterations in the expression levels of key proteins associated with pro-survival signaling pathways, including PI3K, Akt, ERK, NF-κB, Bcl, Bax, cyclin B, CDK4 and CDK6. Importantly, these effects were not reflected in 16HBE normal human bronchial epithelial cells, suggesting a safe range of treatment concentrations between 0 and 10 µM PEITC. In summary, PEITC exhibited significant anticancer effects against human laryngeal cancer cells in vitro with low toxicological impact on normal bronchial epithelial cells. This was achieved through dysregulation of key proteins involved in the occurrence and development of tumors, thereby offering a valuable contribution to future strategies for the treatment and screening of patients with laryngocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Dai M, Wang Y, Chen C, Li F, Xiao B, Chen S and Tao Z: Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells. Oncol Rep 35: 2657-2664, 2016.
APA
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., & Tao, Z. (2016). Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells. Oncology Reports, 35, 2657-2664. https://doi.org/10.3892/or.2016.4689
MLA
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., Tao, Z."Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells". Oncology Reports 35.5 (2016): 2657-2664.
Chicago
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., Tao, Z."Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells". Oncology Reports 35, no. 5 (2016): 2657-2664. https://doi.org/10.3892/or.2016.4689
Copy and paste a formatted citation
x
Spandidos Publications style
Dai M, Wang Y, Chen C, Li F, Xiao B, Chen S and Tao Z: Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells. Oncol Rep 35: 2657-2664, 2016.
APA
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., & Tao, Z. (2016). Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells. Oncology Reports, 35, 2657-2664. https://doi.org/10.3892/or.2016.4689
MLA
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., Tao, Z."Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells". Oncology Reports 35.5 (2016): 2657-2664.
Chicago
Dai, M., Wang, Y., Chen, C., Li, F., Xiao, B., Chen, S., Tao, Z."Phenethyl isothiocyanate induces apoptosis and inhibits cell proliferation and invasion in Hep-2 laryngeal cancer cells". Oncology Reports 35, no. 5 (2016): 2657-2664. https://doi.org/10.3892/or.2016.4689
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