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Article

MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2

Retraction in: /10.3892/or.2021.8106
  • Authors:
    • Can Zhang
    • Feng Long
    • Jun Wan
    • Yihe Hu
    • Hongbo He
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 3275-3284
    |
    Published online on: March 23, 2016
       https://doi.org/10.3892/or.2016.4700
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Abstract

MicroRNAs (miRs) are a class of small non-coding RNAs, and negatively regulate gene expression through directly binding to the 3'-untranslational region (UTR) of their target mRNA, which further leads to translational repression or mRNA degradation. Recently, various miRs have been implicated in the development and progression of osteosarcoma (OS). However, the underlying mechanism has not been fully uncovered. Our study aimed to reveal the exact role of miR-205 in OS, as well as the regulatory mechanism. In this study, we found that the expression of miR-205 was significantly reduced in a total of 34 OS tissue specimens compared to their matched adjacent normal tissues. Besides, it was also remarkably downregulated in OS cell lines (Saos-2, U2OS, SW1353, and MG63) compared to human osteoblast hFOB1.19 cells. Overexpression of miR-205 caused a significant decrease in the proliferation, migration and invasion of MG63 and U2OS cells. Runt-related transcription factor 2 (RUNX2) was further identified as a target gene of miR-205. Moreover, the mRNA and protein expression of RUNX2 was reduced after miR-205 overexpression, but increased after knockdown of miR-205 in MG63 and U2OS cells. Furthermore, overexpression of RUNX2 effectively reversed the suppressive effect of miR-205 on the proliferation, migration, and invasion of MG63 and U2OS cells. The RUNX2 level was significantly increased in OS tissues compared to their matched adjacent normal tissues, as well as in OS cell lines compared to hFOB1.19 cells. In addition, the RUNX2 level was reversely correlated with the miR-205 level in OS tissues. Taken together, our data demonstrate that miR-205 acts as a tumor suppressor in OS via directly targeting RUNX2. Therefore, we suggest that the miR-205/RUNX2 axis may serve as a potential target for the treatment of OS.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Long F, Wan J, Hu Y and He H: MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106. Oncol Rep 35: 3275-3284, 2016.
APA
Zhang, C., Long, F., Wan, J., Hu, Y., & He, H. (2016). MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106. Oncology Reports, 35, 3275-3284. https://doi.org/10.3892/or.2016.4700
MLA
Zhang, C., Long, F., Wan, J., Hu, Y., He, H."MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106". Oncology Reports 35.6 (2016): 3275-3284.
Chicago
Zhang, C., Long, F., Wan, J., Hu, Y., He, H."MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106". Oncology Reports 35, no. 6 (2016): 3275-3284. https://doi.org/10.3892/or.2016.4700
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Long F, Wan J, Hu Y and He H: MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106. Oncol Rep 35: 3275-3284, 2016.
APA
Zhang, C., Long, F., Wan, J., Hu, Y., & He, H. (2016). MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106. Oncology Reports, 35, 3275-3284. https://doi.org/10.3892/or.2016.4700
MLA
Zhang, C., Long, F., Wan, J., Hu, Y., He, H."MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106". Oncology Reports 35.6 (2016): 3275-3284.
Chicago
Zhang, C., Long, F., Wan, J., Hu, Y., He, H."MicroRNA-205 acts as a tumor suppressor in osteosarcoma via targeting RUNX2 Retraction in /10.3892/or.2021.8106". Oncology Reports 35, no. 6 (2016): 3275-3284. https://doi.org/10.3892/or.2016.4700
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